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  1. Microalgae are considered as a source of low pollution and renewable fuel due to their ability to synthesize an abundance of lipids. Conventional methods for lipid quantification are time-consuming and chemica...

    Authors: Yongni Shao, Weimin Gu, Y ating Qiu, Shengfeng Wang, Yan Peng, YiMing Zhu and Songlin Zhuang

    Citation: Biotechnology for Biofuels 2020 13:161

    Content type: Research

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  2. Despite the great potential of marine diatoms in biofuel sector, commercially viable biofuel production from native diatom strain is impractical. Targeted engineering of TAG pathway represents a promising appr...

    Authors: Xiang Wang, Si-Fen Liu, Ruo-Yu Li, Wei-Dong Yang, Jie-Sheng Liu, Carol Sze Ki Lin, Srinivasan Balamurugan and Hong-Ye Li

    Citation: Biotechnology for Biofuels 2020 13:160

    Content type: Research

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  3. Crude glycerol as a promising feedstock for microbial lipid production contains several impurities that make it toxic stress inducer at high amount. Under stress conditions, microorganisms can accumulate l-prolin...

    Authors: Rasool Kamal, Yuxue Liu, Qiang Li, Qitian Huang, Qian Wang, Xue Yu and Zongbao Kent Zhao

    Citation: Biotechnology for Biofuels 2020 13:159

    Content type: Research

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  4. Trichoderma reesei is an ascomycete fungus that has a tremendous capability of secreting extracellular proteins, mostly lignocellulose-degrading enzymes. Although many aspects of the biology of this organism have...

    Authors: Sami Havukainen, Mari Valkonen, Kari Koivuranta and Christopher P. Landowski

    Citation: Biotechnology for Biofuels 2020 13:158

    Content type: Research

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  5. Commercial ethanol fermentation facilities traditionally rely on antibiotics for bacterial contamination control. Here we demonstrate an alternative approach to treat contamination using a novel peptidoglycan ...

    Authors: Shao-Yeh Lu, Kenneth M. Bischoff, Joseph O. Rich, Siqing Liu and Christopher D. Skory

    Citation: Biotechnology for Biofuels 2020 13:157

    Content type: Research

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  6. The integration of first- and second-generation bioethanol processes has the potential to accelerate the establishment of second-generation bioethanol on the market. Cofermenting pretreated wheat straw with a ...

    Authors: Michael Persson, Mats Galbe and Ola Wallberg

    Citation: Biotechnology for Biofuels 2020 13:156

    Content type: Research

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  7. Biomass recalcitrance towards pretreatment and further processing can be related to the compositional and structural features of the biomass. However, the exact role and relative importance to those structural...

    Authors: Dennis Weidener, Murali Dama, Sabine K. Dietrich, Benedict Ohrem, Markus Pauly, Walter Leitner, Pablo Domínguez de María, Philipp M. Grande and Holger Klose

    Citation: Biotechnology for Biofuels 2020 13:155

    Content type: Research

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  8. Similar to biofuels, numerous chemicals produced from petroleum resources can also be made from biomass. In this research we investigate cradle to biorefinery exit gate life cycle impacts of producing acetic a...

    Authors: Erik Budsberg, Rodrigo Morales-Vera, Jordan T. Crawford, Renata Bura and Rick Gustafson

    Citation: Biotechnology for Biofuels 2020 13:154

    Content type: Research

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  9. During the last decades, the impact of hyperthermophiles and their enzymes has been intensively investigated for implementation in various high-temperature biotechnological processes. Biocatalysts of hyperther...

    Authors: Marcel Suleiman, Anna Krüger and Garabed Antranikian

    Citation: Biotechnology for Biofuels 2020 13:153

    Content type: Review

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  10. Phosphopantetheinyl transferase (PPTase) can change the acyl-carrier protein (ACP) from an inactive apo-ACP to an active holo-ACP that plays a key role in fatty acids biosynthesis. Currently, the PPTase has be...

    Authors: Sen Wang, Chuanzeng Lan, Zhuojun Wang, Weijian Wan, Qiu Cui and Xiaojin Song

    Citation: Biotechnology for Biofuels 2020 13:152

    Content type: Research

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  11. Pretreatment processes and subsequent enzymatic hydrolysis are prerequisites to utilize lignocellulosic sugar for fermentation. However, the resulting hydrolysate frequently hinders fermentation processes due ...

    Authors: Jungwoo Yang, Ha Eun Kim, Young Hoon Jung, Jungyeon Kim, Do Hyoung Kim, Adrian R. Walmsley and Kyoung Heon Kim

    Citation: Biotechnology for Biofuels 2020 13:151

    Content type: Research

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  12. Understanding the extracellular electron transport pathways in cyanobacteria is a major factor towards developing biophotovoltaics. Stressing cyanobacteria cells environmentally and then probing changes in phy...

    Authors: Tonny I. Okedi, Adrian C. Fisher and Kamran Yunus

    Citation: Biotechnology for Biofuels 2020 13:150

    Content type: Methodology

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  13. Akebia trifoliata (Thunb.) Koidz may have applications as a new potential source of biofuels owing to its high seed count, seed oil content, and in-field yields. However, the pericarp of A. trifoliata cracks long...

    Authors: Juan Niu, Yaliang Shi, Kunyong Huang, Yicheng Zhong, Jing Chen, Zhimin Sun, Mingbao Luan and Jianhua Chen

    Citation: Biotechnology for Biofuels 2020 13:149

    Content type: Research

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  14. While keeping in view various aspects of energy demand, quest for the renewable energy sources is utmost. Biomass has shown great potential as green energy source with supply of approximately 14% of world tota...

    Authors: Nadeem Tahir, Muhammad Naveed Tahir, Mujeeb Alam, Wang Yi and Quangou Zhang

    Citation: Biotechnology for Biofuels 2020 13:148

    Content type: Research

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  15. Dunaliella salina can produce glycerol under salt stress, and this production can quickly adapt to changes in external salt concentration. Notably, glycerol is an ideal energy source. In recent years, it has been...

    Authors: Ziyi Tang, Xiyue Cao, Yiping Zhang, Jia Jiang, Dairong Qiao, Hui Xu and Yi Cao

    Citation: Biotechnology for Biofuels 2020 13:147

    Content type: Research

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  16. The yield of microalgae biomass is the key to affect the accumulation of fatty acids. A few microalgae can assimilate organic carbon to improve biomass yield. In mixotrophic cultivation, microalgae can use org...

    Authors: Yibo Xiao, Jianying Guo, Huachang Zhu, Anwar Muhammad, Haiteng Deng, Zhangli Hu and Qingyu Wu

    Citation: Biotechnology for Biofuels 2020 13:146

    Content type: Research

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  17. The need to restructure the world’s energy matrix based on fossil fuels and mitigate greenhouse gas emissions stimulated the development of new biobased technologies for renewable energy. One promising and cle...

    Authors: João Gabriel Ribeiro Bueno, Guilherme Borelli, Thamy Lívia Ribeiro Corrêa, Mateus Bernabe Fiamenghi, Juliana José, Murilo de Carvalho, Leandro Cristante de Oliveira, Gonçalo A. G. Pereira and Leandro Vieira dos Santos

    Citation: Biotechnology for Biofuels 2020 13:145

    Content type: Research

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  18. Acid pretreatment is a common strategy used to break down the hemicellulose component of the lignocellulosic biomass to release pentoses, and a subsequent enzymatic hydrolysis step is usually applied to releas...

    Authors: Qing Yang, Yongfu Yang, Ying Tang, Xia Wang, Yunhao Chen, Wei Shen, Yangyang Zhan, Junjie Gao, Bo Wu, Mingxiong He, Shouwen Chen and Shihui Yang

    Citation: Biotechnology for Biofuels 2020 13:144

    Content type: Research

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  19. Lignocellulose is the most abundant and renewable biomass resource on the planet. Lignocellulose can be converted into biofuels and high-value compounds; however, its recalcitrance makes its breakdown a challe...

    Authors: Xin Li, Chao Han, Weiguang Li, Guanjun Chen and Lushan Wang

    Citation: Biotechnology for Biofuels 2020 13:143

    Content type: Research

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  20. Hemicellulose accounts for a significant part of plant biomass, and still poses a barrier to the efficient saccharification of lignocellulose. The recalcitrant part of hemicellulose is a serious impediment to ...

    Authors: Anastasia Zerva, Christina Pentari, Sacha Grisel, Jean-Guy Berrin and Evangelos Topakas

    Citation: Biotechnology for Biofuels 2020 13:142

    Content type: Research

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  21. Biomethanation is a promising solution to upgrade the CH4 content in biogas. This process consists in the injection of H2 into an anaerobic digester, using the capacity of indigenous hydrogenotrophic methanogens ...

    Authors: Lucia Braga Nan, Eric Trably, Gaëlle Santa-Catalina, Nicolas Bernet, Jean-Philippe Delgenès and Renaud Escudié

    Citation: Biotechnology for Biofuels 2020 13:141

    Content type: Research

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  22. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Ying Wang, Ka-Lai Chan, Mohamed Ali Abdel-Rahman, Kenji Sonomoto and Shao-Yuan Leu

    Citation: Biotechnology for Biofuels 2020 13:140

    Content type: Correction

    Published on:

    The original article was published in Biotechnology for Biofuels 2020 13:112

  23. Wood provides an important biomass resource for biofuel production around the world. The radial growth of tree stems is central to biomass production for forestry and biofuels, but it is challenging to dissect...

    Authors: Xiao Han, Yi An, Yangyan Zhou, Chao Liu, Weilun Yin and Xinli Xia

    Citation: Biotechnology for Biofuels 2020 13:139

    Content type: Research

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  24. Consolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic b...

    Authors: Joana T. Cunha, Aloia Romaní, Kentaro Inokuma, Björn Johansson, Tomohisa Hasunuma, Akihiko Kondo and Lucília Domingues

    Citation: Biotechnology for Biofuels 2020 13:138

    Content type: Research

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  25. Trichoderma reesei is one of the best-known cellulolytic organisms, producing large quantities of a complete set of extracellular cellulases and hemicellulases for the degradation of lignocellulosic substances. H...

    Authors: Yun Luo, Mari Valkonen, Raymond E. Jackson, Jonathan M. Palmer, Aditya Bhalla, Igor Nikolaev, Markku Saloheimo and Michael Ward

    Citation: Biotechnology for Biofuels 2020 13:137

    Content type: Research

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  26. Cellobiohydrolase from glycoside hydrolase family 7 is a major component of commercial enzymatic mixtures for lignocellulosic biomass degradation. For many years, Trichoderma reesei Cel7A (TrCel7A) has served as ...

    Authors: Bartłomiej M. Kołaczkowski, Kay S. Schaller, Trine Holst Sørensen, Günther H. J. Peters, Kenneth Jensen, Kristian B. R. M. Krogh and Peter Westh

    Citation: Biotechnology for Biofuels 2020 13:136

    Content type: Research

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  27. Lytic polysaccharide monooxygenases (LPMOs) are often studied in simple models involving activity measurements of a single LPMO or a blend thereof with hydrolytic enzymes towards an insoluble substrate. Howeve...

    Authors: Majid Haddad Momeni, Maria Louise Leth, Claus Sternberg, Erwin Schoof, Maike Wennekers Nielsen, Jesper Holck, Christopher T. Workman, Jakob Blæsbjerg Hoof and Maher Abou Hachem

    Citation: Biotechnology for Biofuels 2020 13:135

    Content type: Research

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  28. Brassica napus is one of the most important oilseed crops, and also an important biofuel plant due to its low air pollution and renewability. Growth period are important traits that affect yield and are crucial f...

    Authors: Tengyue Wang, Lijuan Wei, Jia Wang, Ling Xie, Yang Yang Li, Shuyao Ran, Lanyang Ren, Kun Lu, Jiana Li, Michael P. Timko and Liezhao Liu

    Citation: Biotechnology for Biofuels 2020 13:134

    Content type: Research

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  29. Lupeol exhibits novel physiological and pharmacological activities, such as anticancer and immunity-enhancing activities. However, cytotoxicity remains a challenge for triterpenoid overproduction in microbial ...

    Authors: Jin-Lai Zhang, Qiu-Yan Bai, Yang-Zi Peng, Jie Fan, Cong-Cong Jin, Ying-Xiu Cao and Ying-Jin Yuan

    Citation: Biotechnology for Biofuels 2020 13:133

    Content type: Research

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  30. Fumarate is a multifunctional dicarboxylic acid in the tricarboxylic acid cycle, but microbial engineering for fumarate production is limited by the transmission efficiency of its biosynthetic pathway.

    Authors: Xiulai Chen, Danlei Ma, Jia Liu, Qiuling Luo and Liming Liu

    Citation: Biotechnology for Biofuels 2020 13:132

    Content type: Research

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  31. Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid that has a variety of physiological functions for infant development and human health. Although metabolic engineering wa...

    Authors: Xiao Han, Zhunan Zhao, Ying Wen and Zhi Chen

    Citation: Biotechnology for Biofuels 2020 13:131

    Content type: Research

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  32. Nitrogen (N) starvation in algae induces a variety of structural and metabolic changes including accumulation of triacylglycerol (TAG). Given the promising prospect of using algae as feedstock for biofuel prod...

    Authors: Feifei Xu and Junmin Pan

    Citation: Biotechnology for Biofuels 2020 13:129

    Content type: Research

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  33. Chromochloris zofingiensis, an oleaginous microalga, is a promising feedstock for the co-production of triacylglycerol (TAG)-based biodiesel and the high-value product astaxanthin. To reveal the molecular mechani...

    Authors: Xuemei Mao, Yongmin Lao, Han Sun, Xiaojie Li, Jianfeng Yu and Feng Chen

    Citation: Biotechnology for Biofuels 2020 13:128

    Content type: Research

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  34. Heterotrophic microbial oils are potentially a more sustainable alternative to vegetable or fossil oils for food and fuel applications. However, as almost all work in the area is conducted on the laboratory sc...

    Authors: Felix Abeln, Robert H. Hicks, Hadiza Auta, Mauro Moreno-Beltrán, Luca Longanesi, Daniel A. Henk and Christopher J. Chuck

    Citation: Biotechnology for Biofuels 2020 13:127

    Content type: Research

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  35. High acetic acid tolerance is of major importance in industrial yeast strains used for second-generation bioethanol production, because of the high acetic acid content of lignocellulose hydrolysates. It is als...

    Authors: Marija Stojiljkovic, María R. Foulquié-Moreno and Johan M. Thevelein

    Citation: Biotechnology for Biofuels 2020 13:126

    Content type: Research

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  36. Microbial biorefinery approaches are beginning to define renewable and sustainable routes to clean-burning and non-fossil fuel-derived gaseous alkanes (known as ‘bio-LPG’). The most promising strategies have u...

    Authors: Mohamed Amer, Robin Hoeven, Paul Kelly, Matthew Faulkner, Michael H. Smith, Helen S. Toogood and Nigel S. Scrutton

    Citation: Biotechnology for Biofuels 2020 13:125

    Content type: Research

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  37. The current production of bioethanol based on lignocellulosic biomass (LCB) highly depends on thermostable enzymes and extremophiles owing to less risk of contamination. Thermophilic bacterial cellulases are p...

    Authors: Meena Ganesan, Remitha Mathivani Vinayakamoorthy, Sugitha Thankappan, Iniyakumar Muniraj and Sivakumar Uthandi

    Citation: Biotechnology for Biofuels 2020 13:124

    Content type: Research

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  38. Microbial cell factories are usually engineered and employed for cultivations that combine product synthesis with growth. Such a strategy inevitably invests part of the substrate pool towards the generation of...

    Authors: Enrico Orsi, Ioannis Mougiakos, Wilbert Post, Jules Beekwilder, Marco Dompè, Gerrit Eggink, John van der Oost, Servé W. M. Kengen and Ruud A. Weusthuis

    Citation: Biotechnology for Biofuels 2020 13:123

    Content type: Research

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  39. Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosic autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that s...

    Authors: Xinxing Wu, Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai and Qiang Yong

    Citation: Biotechnology for Biofuels 2020 13:122

    Content type: Research

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  40. Fungal beta-glucosidases (BGs) from glucoside hydrolase family 3 (GH3) are industrially important enzymes, which convert cellooligosaccharides into glucose; the end product of the cellulolytic process. They ar...

    Authors: Malene B. Keller, Trine H. Sørensen, Kristian B. R. M. Krogh, Mark Wogulis, Kim Borch and Peter Westh

    Citation: Biotechnology for Biofuels 2020 13:121

    Content type: Research

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  41. The development of microalgal strains for enhanced biomass and biofuel production has received increased attention. Moreover, strain development via metabolic engineering for commercial production is being con...

    Authors: Seungbeom Seo, Joon Kim, Jun-Woo Lee, Onyou Nam, Kwang Suk Chang and EonSeon Jin

    Citation: Biotechnology for Biofuels 2020 13:120

    Content type: Research

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  42. The use of cyanobacteria and microalgae as cell factories to produce biofuels and added-value bioproducts has received great attention during the last two decades. Important investments have been made by publi...

    Authors: Sandra Isabel González-Morales, Navid Berenice Pacheco-Gutiérrez, Carlos A. Ramírez-Rodríguez, Alethia A. Brito-Bello, Priscila Estrella-Hernández, Luis Herrera-Estrella and Damar L. López-Arredondo

    Citation: Biotechnology for Biofuels 2020 13:119

    Content type: Research

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  43. Lignocellulose is the most abundant biomass on earth with an annual production of about 2 × 1011 tons. It is an inedible renewable carbonaceous resource that is very rich in pentose and hexose sugars. The ability...

    Authors: Jean Marie Francois, Ceren Alkim and Nicolas Morin

    Citation: Biotechnology for Biofuels 2020 13:118

    Content type: Review

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  44. Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO2 by photosynt...

    Authors: Wenyang Wu, Wei Du, Ruth Perez Gallego, Klaas J. Hellingwerf, Aniek D. van der Woude and Filipe Branco dos Santos

    Citation: Biotechnology for Biofuels 2020 13:117

    Content type: Research

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  45. Global resource reallocation is an established critical strategy through which organisms deal with environmental stress. The regulation of intracellular lipid storage or utilization is one of the most importan...

    Authors: Hengqian Lu, Haiqin Chen, Xin Tang, Qin Yang, Hao Zhang, Yong Q. Chen and Wei Chen

    Citation: Biotechnology for Biofuels 2020 13:116

    Content type: Research

    Published on:

  46. N-Acetyl glucosamine (GlcNAc) and N-Acetyl chitooligosaccharides (N-Acetyl COSs) exhibit many biological activities, and have been widely used in the pharmaceutical, agriculture, food, and chemical industries. Pa...

    Authors: Alei Zhang, Xiaofang Mo, Ning Zhou, Yingying Wang, Guoguang Wei, Jie Chen, Kequan Chen and Pingkai Ouyang

    Citation: Biotechnology for Biofuels 2020 13:115

    Content type: Research

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  47. Aldehyde decarbonylases (ADs), which convert acyl aldehydes into alkanes, supply promising solution for producing alkanes from renewable feedstock. However the instability of ADs impedes their further applicat...

    Authors: Yilan Liu, Jinjin Chen, Anna N. Khusnutdinova, Kevin Correia, Patrick Diep, Khorcheska A. Batyrova, Kayla Nemr, Robert Flick, Peter Stogios, Alexander F. Yakunin and Radhakrishnan Mahadevan

    Citation: Biotechnology for Biofuels 2020 13:114

    Content type: Research

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  48. Xylose is the most prevalent sugar available in hemicellulose fraction of lignocellulosic biomass (LCB) and of great interest for the green economy. Unfortunately, most of the cell factories cannot inherently ...

    Authors: Ashish A. Prabhu, Rodrigo Ledesma-Amaro, Carol Sze Ki Lin, Frederic Coulon, Vijay Kumar Thakur and Vinod Kumar

    Citation: Biotechnology for Biofuels 2020 13:113

    Content type: Research

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  49. The simultaneous and effective conversion of both pentose and hexose in fermentation is a critical and challenging task toward the lignocellulosic economy. This study aims to investigate the feasibility of an ...

    Authors: Ying Wang, Ka-Lai Chan, Mohamed Ali Abdel-Rahman, Kenji Sonomoto and Shao-Yuan Leu

    Citation: Biotechnology for Biofuels 2020 13:112

    Content type: Research

    Published on:

    The Correction to this article has been published in Biotechnology for Biofuels 2020 13:140