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  1. Consolidated bioprocessing (CBP) technique is a promising strategy for biorefinery construction, producing bulk chemicals directly from plant biomass without extra hydrolysis steps. Fixing and channeling CO2 into...

    Authors: Jingen Li, Bingchen Chen, Shuying Gu, Zhen Zhao, Qian Liu, Tao Sun, Yongli Zhang, Taju Wu, Defei Liu, Wenliang Sun and Chaoguang Tian

    Citation: Biotechnology for Biofuels 2021 14:186

    Content type: Research

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  2. Recent research articles indicate that direct interspecies electron transfer (DIET) is an alternative metabolic route for methanogenic archaea that improves microbial methane productivity. It has been shown th...

    Authors: Kerstin Heitkamp, Adriel Latorre-Pérez, Sven Nefigmann, Helena Gimeno-Valero, Cristina Vilanova, Efri Jahmad and Christian Abendroth

    Citation: Biotechnology for Biofuels 2021 14:185

    Content type: Research

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  3. The green microalga Chromochloris zofingiensis is capable of producing high levels of triacylglycerol rich in C18 unsaturated fatty acids (UFAs). FA desaturation degree is regulated by FA desaturases (FADs). Neve...

    Authors: Tao Wu, Lihua Yu, Yu Zhang and Jin Liu

    Citation: Biotechnology for Biofuels 2021 14:184

    Content type: Research

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  4. Endo-β-1,4-galactanases are glycoside hydrolases (GH) from the GH53 family belonging to the largest clan of GHs, clan GH-A. GHs are ubiquitous and involved in a myriad of biological functions as well as being ...

    Authors: Sebastian J. Muderspach, Folmer Fredslund, Verena Volf, Jens-Christian Navarro Poulsen, Thomas H. Blicher, Mads Hartvig Clausen, Kim Krighaar Rasmussen, Kristian B. R. M. Krogh, Kenneth Jensen and Leila Lo Leggio

    Citation: Biotechnology for Biofuels 2021 14:183

    Content type: Research

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  5. Bio-hydrogen production via dark fermentation of low-value waste is a potent and simple mean of recovering energy, maximising the harvesting of reducing equivalents to produce the cleanest fuel amongst renewab...

    Authors: M. Arizzi, S. Morra, G. Gilardi, M. Pugliese, M. L. Gullino and F. Valetti

    Citation: Biotechnology for Biofuels 2021 14:182

    Content type: Research

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  6. Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates h...

    Authors: Zhi-Peng Wang, Xin-Yue Zhang, Yan Ma, Jing-Run Ye, Jing Jiang, Hai-Ying Wang and Wei Chen

    Citation: Biotechnology for Biofuels 2021 14:181

    Content type: Research

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  7. Microalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, e...

    Authors: M. Giovannoni, I. Larini, V. Scafati, A. Scortica, M. Compri, D. Pontiggia, G. Zapparoli, N. Vitulo, M. Benedetti and B. Mattei

    Citation: Biotechnology for Biofuels 2021 14:180

    Content type: Research

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  8. Environmental factors, such as weather extremes, have the potential to cause adverse effects on plant biomass quality and quantity. Beyond adversely affecting feedstock yield and composition, which have been e...

    Authors: Meenaa Chandrasekar, Leela Joshi, Karleigh Krieg, Sarvada Chipkar, Emily Burke, Derek J. Debrauske, Kurt D. Thelen, Trey K. Sato and Rebecca G. Ong

    Citation: Biotechnology for Biofuels 2021 14:179

    Content type: Research

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  9. In terms of global demand, rapeseed is the third-largest oilseed crop after soybeans and palm, which produces vegetable oil for human consumption and biofuel for industrial production. Roots are vital organs f...

    Authors: Keqi Li, Jie Wang, Lieqiong Kuang, Ze Tian, Xinfa Wang, Xiaoling Dun, Jinxing Tu and Hanzhong Wang

    Citation: Biotechnology for Biofuels 2021 14:178

    Content type: Research

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  10. To create an ideotype woody bioenergy crop with desirable growth and biomass properties, we utilized the viral 2A-meidated bicistronic expression strategy to express both PtrMYB3 (MYB46 ortholog of Populus tricho...

    Authors: Jin-Seong Cho, Min-Ha Kim, Eun-Kyung Bae, Young-Im Choi, Hyung-Woo Jeon, Kyung-Hwan Han and Jae-Heung Ko

    Citation: Biotechnology for Biofuels 2021 14:177

    Content type: Research

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  11. The efficiency of biological systems as an option for pretreating lignocellulosic biomass has to be improved to make the process practical. Fungal treatment with manganese (Mn) addition for improving lignocell...

    Authors: Xiao Fu, Jialong Zhang, Xiangyu Gu, Hongbo Yu and Shulin Chen

    Citation: Biotechnology for Biofuels 2021 14:176

    Content type: Research

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  12. Lignification of secondary cell walls is a major factor conferring recalcitrance of lignocellulosic biomass to deconstruction for fuels and chemicals. Genetic modification can reduce lignin content and enhance...

    Authors: Hasi Yu, Chang Liu and Richard A. Dixon

    Citation: Biotechnology for Biofuels 2021 14:175

    Content type: Methodology

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  13. Isobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO2 into bio-based fuels have drawn considerable attention due to their lower feedstock cost and more ecofrien...

    Authors: Xiao-Xi Wu, Jian-Wei Li, Su-Fang Xing, Hui-Ting Chen, Chao Song, Shu-Guang Wang and Zhen Yan

    Citation: Biotechnology for Biofuels 2021 14:174

    Content type: Research

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  14. The fermentation of lignocellulose hydrolysates to ethanol requires robust xylose-capable Saccharomyces cerevisiae strains able to operate in the presence of microbial inhibitory stresses. This study aimed at dev...

    Authors: Bianca A. Brandt, Maria D. P. García-Aparicio, Johann F. Görgens and Willem H. van Zyl

    Citation: Biotechnology for Biofuels 2021 14:173

    Content type: Research

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  15. Over the last few years, valorization of lignocellulosic biomass has been expanded beyond the production of second-generation biofuels to the synthesis of numerous platform chemicals to be used instead of thei...

    Authors: Grigorios Dedes, Anthi Karnaouri, Asimina A. Marianou, Konstantinos G. Kalogiannis, Chrysoula M. Michailof, Angelos A. Lappas and Evangelos Topakas

    Citation: Biotechnology for Biofuels 2021 14:172

    Content type: Research

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  16. Sugarcane bagasse (SCB) is an abundant feedstock for second-generation bioethanol production. This complex biomass requires an array of carbohydrate active enzymes (CAZymes), mostly from filamentous fungi, for...

    Authors: Olusola A. Ogunyewo, Pooja Upadhyay, Girish H. Rajacharya, Omoaruemike E. Okereke, Laura Faas, Leonardo D. Gómez, Simon J. McQueen-Mason and Syed Shams Yazdani

    Citation: Biotechnology for Biofuels 2021 14:171

    Content type: Research

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  17. Biogas can be upgraded to methane biologically by adding H2 to biogas reactors. The process is called biological methanation (BM) and can be done in situ in a regular biogas reactor or the biogas can be transferr...

    Authors: Radziah Wahid and Svein Jarle Horn

    Citation: Biotechnology for Biofuels 2021 14:170

    Content type: Research

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  18. Filamentous fungi are excellent lignocellulose degraders, which they achieve through producing carbohydrate active enzymes (CAZymes). CAZyme production is highly orchestrated and gene expression analysis has g...

    Authors: Raphael Gabriel, Rebecca Mueller, Lena Floerl, Cynthia Hopson, Simon Harth, Timo Schuerg, Andre Fleissner and Steven W. Singer

    Citation: Biotechnology for Biofuels 2021 14:169

    Content type: Research

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  19. Rhodospirillum rubrum is a purple non-sulphur bacterium that produces H2 by photofermentation of several organic compounds or by water gas-shift reaction during CO fermentation. Successful strategies for both pro...

    Authors: Alberto Rodríguez, Natalia Hernández-Herreros, José L. García and M. Auxiliadora Prieto

    Citation: Biotechnology for Biofuels 2021 14:168

    Content type: Research

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  20. Plants inherently display a rich diversity in cell wall chemistry, as they synthesize an array of polysaccharides along with lignin, a polyphenolic that can vary dramatically in subunit composition and interun...

    Authors: Lisanne de Vries, Sydne Guevara-Rozo, MiJung Cho, Li-Yang Liu, Scott Renneckar and Shawn D. Mansfield

    Citation: Biotechnology for Biofuels 2021 14:167

    Content type: Review

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  21. As the fifth major cereal crop originated from Africa, sorghum (Sorghum bicolor) has become a key C4 model organism for energy plant research. With the development of high-throughput detection technologies for va...

    Authors: Yuanming Liu, Zhonghuang Wang, Xiaoyuan Wu, Junwei Zhu, Hong Luo, Dongmei Tian, Cuiping Li, Jingchu Luo, Wenming Zhao, Huaiqing Hao and Hai-Chun Jing

    Citation: Biotechnology for Biofuels 2021 14:165

    Content type: Research

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  22. Biomass recalcitrance is governed by various molecular and structural factors but the interplay between these multiscale factors remains unclear. In this study, hot water pretreatment (HWP) was applied to maiz...

    Authors: Amandine Leroy, Xavier Falourd, Loïc Foucat, Valérie Méchin, Fabienne Guillon and Gabriel Paës

    Citation: Biotechnology for Biofuels 2021 14:164

    Content type: Research

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  23. As a potential source of polyunsaturated fatty acids (PUFA), Schizochytrium sp. has been widely used in industry for PUFA production. Polyketide synthase (PKS) cluster is supposed to be the primary way of PUFA sy...

    Authors: Yanyan Shi, Zhen Chen, Yixin Li, Xingyu Cao, Lijie Yang, Yiyuan Xu, Zhipeng Li and Ning He

    Citation: Biotechnology for Biofuels 2021 14:163

    Content type: Research

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  24. As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin d...

    Authors: Tangwu Cui, Bo Yuan, Haiwei Guo, Hua Tian, Weimin Wang, Yingqun Ma, Changzhi Li and Qiang Fei

    Citation: Biotechnology for Biofuels 2021 14:162

    Content type: Research

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  25. Fungal glucose dehydrogenases (GDHs) are FAD-dependent enzymes belonging to the glucose-methanol-choline oxidoreductase superfamily. These enzymes are classified in the “Auxiliary Activity” family 3 (AA3) of t...

    Authors: Gabriele Cerutti, Elena Gugole, Linda Celeste Montemiglio, Annick Turbé-Doan, Dehbia Chena, David Navarro, Anne Lomascolo, François Piumi, Cécile Exertier, Ida Freda, Beatrice Vallone, Eric Record, Carmelinda Savino and Giuliano Sciara

    Citation: Biotechnology for Biofuels 2021 14:161

    Content type: Research

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  26. Population increase and industrialization has resulted in high energy demand and consumptions, and presently, fossil fuels are the major source of staple energy, supplying 80% of the entire consumption. This h...

    Authors: Kehinde Oladoke Olatunji, Noor A. Ahmed and Oyetola Ogunkunle

    Citation: Biotechnology for Biofuels 2021 14:159

    Content type: Review

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  27. Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are ...

    Authors: Shannon M. Hoffman, Maria Alvarez, Gilad Alfassi, Dmitry M. Rein, Sergio Garcia-Echauri, Yachin Cohen and José L. Avalos

    Citation: Biotechnology for Biofuels 2021 14:157

    Content type: Research

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  28. Production of biodiesel from renewable sources such as inedible vegetable oils by enzymatic catalysis has been a hotspot but remains a challenge on the efficient use of an enzyme. COFs (Covalent Organic Framew...

    Authors: Zi-Wen Zhou, Chun-Xian Cai, Xiu Xing, Jun Li, Zu-E. Hu, Zong-Bo Xie, Na Wang and Xiao-Qi Yu

    Citation: Biotechnology for Biofuels 2021 14:156

    Content type: Research

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  29. Saccharomyces cerevisiae is widely used in traditional brewing and modern fermentation industries to produce biofuels, chemicals and other bioproducts, but challenged by various harsh industrial conditions, such ...

    Authors: Yanfang Liu, Yuping Lin, Yufeng Guo, Fengli Wu, Yuanyuan Zhang, Xianni Qi, Zhen Wang and Qinhong Wang

    Citation: Biotechnology for Biofuels 2021 14:155

    Content type: Research

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  30. The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role in biomass degradation, nutrient recycling and ecosystem fu...

    Authors: Sivasamy Sethupathy, Gabriel Murillo Morales, Yixuan Li, Yongli Wang, Jianxiong Jiang, Jianzhong Sun and Daochen Zhu

    Citation: Biotechnology for Biofuels 2021 14:154

    Content type: Review

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  31. Sugarcane processing roughly generates 54 million tonnes sugarcane bagasse (SCB)/year, making SCB an important material for upgrading to value-added molecules. In this study, an integrated scheme was developed...

    Authors: Leila Khaleghipour, Javier A. Linares-Pastén, Hamid Rashedi, Seyed Omid Ranaei Siadat, Andrius Jasilionis, Said Al-Hamimi, Roya R. R. Sardari and Eva Nordberg Karlsson

    Citation: Biotechnology for Biofuels 2021 14:153

    Content type: Research

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  32. Sugarcane is an essential crop for sugar and ethanol production. Immediate processing of sugarcane is necessary after harvested because of rapid sucrose losses and deterioration of stalks. This study was condu...

    Authors: Na Peng, Ziting Yao, Ziting Wang, Jiangfeng Huang, Muhammad Tahir Khan, Baoshan Chen and Muqing Zhang

    Citation: Biotechnology for Biofuels 2021 14:152

    Content type: Research

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  33. Hydrogen peroxide–acetic acid (HPAA) is widely used in pretreatment of lignocellulose because it has a good capability in selective delignification. However, high concentration (more than 60%) of HPAA increase...

    Authors: Peiyao Wen, Ying Zhang, Junjun Zhu, Yong Xu and Junhua Zhang

    Citation: Biotechnology for Biofuels 2021 14:151

    Content type: Research

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  34. Ascomycetous yeasts from the kingdom fungi inhabit every biome in nature. While filamentous fungi have been studied extensively regarding their enzymatic degradation of the complex polymers comprising lignocel...

    Authors: Jonas L. Ravn, Martin K. M. Engqvist, Johan Larsbrink and Cecilia Geijer

    Citation: Biotechnology for Biofuels 2021 14:150

    Content type: Research

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  35. In biological cells, promoters drive gene expression by specific binding of RNA polymerase. They determine the starting position, timing and level of gene expression. Therefore, rational fine-tuning of promote...

    Authors: Yu Zhao, Shiqi Liu, Zhihui Lu, Baixiang Zhao, Shuhui Wang, Cuiying Zhang, Dongguang Xiao, Jee Loon Foo and Aiqun Yu

    Citation: Biotechnology for Biofuels 2021 14:149

    Content type: Research

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  36. Lipomyces starkeyi has been widely regarded as a promising oleaginous yeast with broad industrial application prospects because of its wide substrate spectrum, good adaption to fermentation inhibitors, excellent ...

    Authors: Wei Zhou, Yanan Wang, Junlu Zhang, Man Zhao, Mou Tang, Wenting Zhou and Zhiwei Gong

    Citation: Biotechnology for Biofuels 2021 14:148

    Content type: Research

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  37. Plant monoterpenoids with structural diversities have extensive applications in food, cosmetics, pharmaceuticals, and biofuels. Due to the strong dependence on the geographical locations and seasonal annual gr...

    Authors: Dengwei Lei, Zetian Qiu, Jianjun Qiao and Guang-Rong Zhao

    Citation: Biotechnology for Biofuels 2021 14:147

    Content type: Review

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  38. The model ethanologenic bacterium Zymomonas mobilis has many advantages for diverse biochemical production. Although the impact of temperature especially high temperature on the growth and ethanol production of Z...

    Authors: Runxia Li, Wei Shen, Yongfu Yang, Jun Du, Mian Li and Shihui Yang

    Citation: Biotechnology for Biofuels 2021 14:146

    Content type: Research

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  39. Succinic acid (SA) is a crucial metabolic intermediate and platform chemical. Development of biobased processes to achieve sustainable SA production has attracted more and more attention in biotechnology indus...

    Authors: Zhennan Jiang, Zhiyong Cui, Ziwei Zhu, Yinghang Liu, Ya-jie Tang, Jin Hou and Qingsheng Qi

    Citation: Biotechnology for Biofuels 2021 14:145

    Content type: Research

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  40. Identifying lignocellulose recalcitrant factors and exploring their genetic properties are essential for enhanced biomass enzymatic saccharification in bioenergy crops. Despite genetic modification of major wa...

    Authors: Zhen Hu, Youmei Wang, Jingyuan Liu, Yuqi Li, Yanting Wang, Jiangfeng Huang, Yuanhang Ai, Peng Chen, Yuqing He, Muhammad Nauman Aftab, Lingqiang Wang and Liangcai Peng

    Citation: Biotechnology for Biofuels 2021 14:144

    Content type: Research

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  41. During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. H...

    Authors: Wenqian Lin, Jinlai Yang, Yayue Zheng, Caoxing Huang and Qiang Yong

    Citation: Biotechnology for Biofuels 2021 14:143

    Content type: Research

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  42. In plants, a large diversity of polysaccharides comprise the cell wall. Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions ...

    Authors: William J. Barnes, Sabina Koj, Ian M. Black, Stephanie A. Archer-Hartmann, Parastoo Azadi, Breeanna R. Urbanowicz, Maria J. Peña and Malcolm A. O’Neill

    Citation: Biotechnology for Biofuels 2021 14:142

    Content type: Methodology

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  43. Salix (willow) species represent an important source of bioenergy and offer great potential for producing biofuels. Salix spp. like many hardwoods, produce tension wood (TW) characterized by special fibres (G-fib...

    Authors: Jie Gao, Mohamed Jebrane, Nasko Terziev and Geoffrey Daniel

    Citation: Biotechnology for Biofuels 2021 14:141

    Content type: Research

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  44. The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses. In the past 5 years, exciting possibilities have emerged in the engineering ...

    Authors: Madalen Robert, Julian Waldhauer, Fabian Stritt, Bo Yang, Markus Pauly and Cătălin Voiniciuc

    Citation: Biotechnology for Biofuels 2021 14:140

    Content type: Research

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  45. Biomass valorization has been suggested as a sustainable alternative to petroleum-based energy and commodities. In this context, the copper radical oxidases (CROs) from Auxiliary Activity Family 5/Subfamily 2 ...

    Authors: Maria Cleveland, Mickael Lafond, Fan Roderick Xia, Ryan Chung, Paul Mulyk, Jason E. Hein and Harry Brumer

    Citation: Biotechnology for Biofuels 2021 14:138

    Content type: Research

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  46. Cellulose is not only a common component in vascular plants, but also has great economic benefits for paper, wood, and industrial products. In addition, its biosynthesis is highly regulated by carbohydrate met...

    Authors: Jing Su, Chunxia Zhang, Lingcheng Zhu, Nanxiang Yang, Jingjing Yang, Baiquan Ma, Fengwang Ma and Mingjun Li

    Citation: Biotechnology for Biofuels 2021 14:137

    Content type: Research

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