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  1. Research

    Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis

    Synechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found to be 99.8% identical to S. elongatus 7942 yet it grows twice as fas...

    Mary H. Abernathy, Jingjie Yu, Fangfang Ma, Michelle Liberton, Justin Ungerer, Whitney D. Hollinshead, Saratram Gopalakrishnan, Lian He, Costas D. Maranas, Himadri B. Pakrasi, Doug K. Allen and Yinjie J. Tang

    Biotechnology for Biofuels 2017 10:273

    Published on: 16 November 2017

  2. Research

    Production of the versatile cellulase for cellulose bioconversion and cellulase inducer synthesis by genetic improvement of Trichoderma reesei

    The enzymes for efficient hydrolysis of lignocellulosic biomass are a major factor in the development of an economically feasible cellulose bioconversion process. Up to now, low hydrolysis efficiency and high ...

    Jia Gao, Yuanchao Qian, Yifan Wang, Yinbo Qu and Yaohua Zhong

    Biotechnology for Biofuels 2017 10:272

    Published on: 15 November 2017

  3. Research

    Xylose induces cellulase production in Thermoascus aurantiacus

    Lignocellulosic biomass is an important resource for renewable production of biofuels and bioproducts. Enzymes that deconstruct this biomass are critical for the viability of biomass-based biofuel production p...

    Timo Schuerg, Jan-Philip Prahl, Raphael Gabriel, Simon Harth, Firehiwot Tachea, Chyi-Shin Chen, Matthew Miller, Fabrice Masson, Qian He, Sarah Brown, Mona Mirshiaghi, Ling Liang, Lauren M. Tom, Deepti Tanjore, Ning Sun, Todd R. Pray…

    Biotechnology for Biofuels 2017 10:271

    Published on: 15 November 2017

  4. Review

    Current status and perspectives of genome editing technology for microalgae

    Genome editing techniques are critical for manipulating genes not only to investigate their functions in biology but also to improve traits for genetic engineering in biotechnology. Genome editing has been gre...

    Seungjib Jeon, Jong-Min Lim, Hyung-Gwan Lee, Sung-Eun Shin, Nam Kyu Kang, Youn-Il Park, Hee-Mock Oh, Won-Joong Jeong, Byeong-ryool Jeong and Yong Keun Chang

    Biotechnology for Biofuels 2017 10:267

    Published on: 14 November 2017

  5. Research

    Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance

    The Bacillus subtilis endo-β-1,4-glucanase (BsCel5A) hydrolyzes β-1,3-1,4-linked glucan, and the enzyme includes a family 3 carbohydrate-binding module (CBM3) that binds β-1,4-linked glucan.

    Raquel Fonseca-Maldonado, Luana P. Meleiro, Luís F. S. Mendes, Luana F. Alves, Sibeli Carli, Lucas D. Morero, Luis G. M. Basso, Antonio J. Costa-Filho and Richard J. Ward

    Biotechnology for Biofuels 2017 10:269

    Published on: 14 November 2017

  6. Research

    Genomics and prevalence of bacterial and archaeal isolates from biogas-producing microbiomes

    To elucidate biogas microbial communities and processes, the application of high-throughput DNA analysis approaches is becoming increasingly important. Unfortunately, generated data can only partialy be interp...

    Irena Maus, Andreas Bremges, Yvonne Stolze, Sarah Hahnke, Katharina G. Cibis, Daniela E. Koeck, Yong S. Kim, Jana Kreubel, Julia Hassa, Daniel Wibberg, Aaron Weimann, Sandra Off, Robbin Stantscheff, Vladimir V. Zverlov, Wolfgang H. Schwarz, Helmut König…

    Biotechnology for Biofuels 2017 10:264

    Published on: 13 November 2017

  7. Research

    Combined genome and transcriptome sequencing to investigate the plant cell wall degrading enzyme system in the thermophilic fungus Malbranchea cinnamomea

    Genome and transcriptome sequencing has greatly facilitated the understanding of biomass-degrading mechanisms in a number of fungal species. The information obtained enables the investigation and discovery of ...

    Silvia Hüttner, Thanh Thuy Nguyen, Zoraide Granchi, Thomas Chin-A-Woeng, Dag Ahrén, Johan Larsbrink, Vu Nguyen Thanh and Lisbeth Olsson

    Biotechnology for Biofuels 2017 10:265

    Published on: 13 November 2017

  8. Research

    Enhancing biogas plant production using pig manure and corn silage by adding wheat straw processed with liquid hot water and steam explosion

    Pig manure utilization and valorization is an important topic with tightening regulations focused on ecological and safety issues. By itself pig manure is a poor substrate for biogas production because of its ...

    Michał Gaworski, Sławomir Jabłoński, Izabela Pawlaczyk-Graja, Rafał Ziewiecki, Piotr Rutkowski, Anna Wieczyńska, Roman Gancarz and Marcin Łukaszewicz

    Biotechnology for Biofuels 2017 10:259

    Published on: 9 November 2017

  9. Research

    Cell growth and lipid accumulation of a microalgal mutant Scenedesmus sp. Z-4 by combining light/dark cycle with temperature variation

    The light/dark cycle is one of the most important factors affecting the microalgal growth and lipid accumulation. Biomass concentration and lipid productivity could be enhanced by optimization of light/dark cy...

    Chao Ma, Yan-Bo Zhang, Shih-Hsin Ho, De-Feng Xing, Nan-Qi Ren and Bing-Feng Liu

    Biotechnology for Biofuels 2017 10:260

    Published on: 9 November 2017

  10. Research

    Techno-economic and resource analysis of hydroprocessed renewable jet fuel

    Biomass-derived jet fuel is an alternative jet fuel (AJF) showing promise of reducing the dependence on fossil fuel and greenhouse gas emissions. Hydroprocessed esters and fatty acids (HEFA) concept is also kn...

    Ling Tao, Anelia Milbrandt, Yanan Zhang and Wei-Cheng Wang

    Biotechnology for Biofuels 2017 10:261

    Published on: 9 November 2017

  11. Research

    A novel, highly efficient β-glucosidase with a cellulose-binding domain: characterization and properties of native and recombinant proteins

    Cellulose, the most abundant biopolymer on earth, is an alternative for fossil fuels as a renewable feedstock for the production of second-generation biofuels and other chemicals. The discovery of novel, highl...

    J. A. Méndez-Líter, J. Gil-Muñoz, M. Nieto-Domínguez, J. Barriuso, L. I. de Eugenio and M. J. Martínez

    Biotechnology for Biofuels 2017 10:256

    Published on: 6 November 2017

  12. Research

    Targeted metatranscriptomics of compost-derived consortia reveals a GH11 exerting an unusual exo-1,4-β-xylanase activity

    Using globally abundant crop residues as a carbon source for energy generation and renewable chemicals production stand out as a promising solution to reduce current dependency on fossil fuels. In nature, such...

    Bruno L. Mello, Anna M. Alessi, Diego M. Riaño-Pachón, Eduardo R. deAzevedo, Francisco E. G. Guimarães, Melissa C. Espirito Santo, Simon McQueen-Mason, Neil C. Bruce and Igor Polikarpov

    Biotechnology for Biofuels 2017 10:254

    Published on: 2 November 2017

  13. Research

    Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis

    Saccharification is the most crucial and cost-intensive process in second generation biofuel production. The deficiency of β-glucosidase in commercial enzyme leads to incomplete biomass hydrolysis. The decompo...

    Rameshwar Tiwari, Puneet Kumar Singh, Surender Singh, Pawan K. S. Nain, Lata Nain and Pratyoosh Shukla

    Biotechnology for Biofuels 2017 10:246

    Published on: 30 October 2017

  14. Research

    A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis

    Like a number of anaerobic and cellulolytic Gram-positive bacteria, the model microorganism Ruminiclostridium cellulolyticum produces extracellular multi-enzymatic complexes called cellulosomes, which efficiently...

    Aurélie Fosses, Maria Maté, Nathalie Franche, Nian Liu, Yann Denis, Romain Borne, Pascale de Philip, Henri-Pierre Fierobe and Stéphanie Perret

    Biotechnology for Biofuels 2017 10:250

    Published on: 30 October 2017

  15. Research

    Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes

    Enterobacter aerogenes is a facultative anaerobe and is one of the most widely studied bacterial strains because of its ability to use a variety of substrates, to produce hydrogen at a...

    Yan Wu, Yaqiao Hao, Xuan Wei, Qi Shen, Xuanwei Ding, Liyan Wang, Hongxin Zhao and Yuan Lu

    Biotechnology for Biofuels 2017 10:248

    Published on: 30 October 2017

  16. Research

    Comparative characterization of all cellulosomal cellulases from Clostridium thermocellum reveals high diversity in endoglucanase product formation essential for complex activity

    Clostridium thermocellum is a paradigm for efficient cellulose degradation and a promising organism for the production of second generation biofuels. It owes its high degradation rate ...

    Benedikt Leis, Claudia Held, Fabian Bergkemper, Katharina Dennemarck, Robert Steinbauer, Alarich Reiter, Matthias Mechelke, Matthias Moerch, Sigrid Graubner, Wolfgang Liebl, Wolfgang H. Schwarz and Vladimir V. Zverlov

    Biotechnology for Biofuels 2017 10:240

    Published on: 23 October 2017

  17. Research

    Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts

    Economical conversion of lignocellulosic biomass into biofuels and bioproducts is central to the establishment of a robust bioeconomy. This requires a conversion host that is able to both efficiently assimilat...

    Junko Yaegashi, James Kirby, Masakazu Ito, Jian Sun, Tanmoy Dutta, Mona Mirsiaghi, Eric R. Sundstrom, Alberto Rodriguez, Edward Baidoo, Deepti Tanjore, Todd Pray, Kenneth Sale, Seema Singh, Jay D. Keasling, Blake A. Simmons, Steven W. Singer…

    Biotechnology for Biofuels 2017 10:241

    Published on: 23 October 2017

  18. Research

    Sustainable carbon sources for microbial organic acid production with filamentous fungi

    The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation a...

    Stefan Dörsam, Jana Fesseler, Olga Gorte, Thomas Hahn, Susanne Zibek, Christoph Syldatk and Katrin Ochsenreither

    Biotechnology for Biofuels 2017 10:242

    Published on: 23 October 2017

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