- Open Access
Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass
Biotechnology for Biofuels volume 10, Article number: 14 (2017)
Clostridium thermocellum is capable of solubilizing and converting lignocellulosic biomass into ethanol. Although much of the work-to-date has centered on characterizing this microbe’s growth on model cellulosic substrates, such as cellobiose, Avicel, or filter paper, it is vitally important to understand its metabolism on more complex, lignocellulosic substrates to identify relevant industrial bottlenecks that could undermine efficient biofuel production. To this end, we have examined a time course progression of C. thermocellum grown on switchgrass to assess the metabolic and protein changes that occur during the conversion of plant biomass to ethanol.
The most striking feature of the metabolome was the observed accumulation of long-chain, branched fatty acids over time, implying an adaptive restructuring of C. thermocellum’s cellular membrane as the culture progresses. This is undoubtedly a response to the gradual accumulation of lignocellulose-derived inhibitory compounds as the organism deconstructs the switchgrass to access the embedded cellulose. Corroborating the metabolomics data, proteomic analysis revealed a corresponding time-dependent increase in various enzymes, including those involved in the interconversion of branched amino acids valine, leucine, and isoleucine to iso- and anteiso-fatty acid precursors. Additionally, the metabolic accumulation of hemicellulose-derived sugars and sugar alcohols concomitant with increased abundance of enzymes involved in C5 sugar metabolism/pentose phosphate pathway indicates that C. thermocellum shifts glycolytic intermediates to alternate pathways to modulate overall carbon flux in response to C5 sugar metabolites that increase during lignocellulose deconstruction.
Integrated omic platforms provided complementary systems biological information that highlight C. thermocellum’s specific response to cytotoxic inhibitors released during the deconstruction and utilization of switchgrass. These additional viewpoints allowed us to fully realize the level to which the organism adapts to an increasingly challenging culture environment—information that will prove critical to C. thermocellum’s industrial efficacy.
Switchgrass is a perennial, warm-season, C4 grass that is one of the dominant grasses in North America. It is a promising second-generation bioenergy feedstock due, in part, to its hardiness, high yields, low fertilizer requirements', and drought tolerance, and, thus, it has the potential to augment or replace existing starch-based processes for biofuel production . Compared to first-generation biofuels, where added enzymes are used to deconstruct corn starch to dextrose for fermentation to ethanol by yeast, second-generation biofuels target the vast energy reserves stored in plant cell walls. Unlike starch, plant cell walls are generally difficult to deconstruct, since they consist of large, intertwined, recalcitrant biopolymers of C5 sugars (hemicellulose), C6 sugars (cellulose), and lignin [2, 3]. Accessing this reservoir of chemical energy requires the concerted action of multiple enzymes with diverse catalytic activities —a bioengineering feat inherent to various cellulolytic microorganisms capable of solubilizing and ultimately consuming naturally abundant cell wall polysaccharides .
Clostridium thermocellum is an industrially relevant, cellulolytic microbe that efficiently deconstructs lignocellulosic biomass into sugars, which are fermented into ethanol and other products. As an anaerobic thermophile, this Gram-positive bacterium can be found in natural environments where cellulose degradation actively occurs (e.g., compost piles). It produces large extracellular enzyme complexes called cellulosomes that are predominantly tethered to the cell surface but can exist as free entities, enabling the efficient solubilization and deconstruction of lignocellulose to simpler sugars [6, 7]. Paired with the organism’s innate ability to ferment sugar to ethanol, the presence of cellulosomes makes C. thermocellum, an ideal candidate for consolidated bioprocessing (CBP), a “one-pot” industrial process whereby lignocellulosic biomass is converted directly into biofuel .
The cellulosome, replete with feedstock-optimized carbohydrate-active enzymes (CAZymes) , directs the conversion of cellulose to small, importable cellodextrins . Intracellularly, these cellodextrins are further broken down into cellobiose and finally glucose, which is ultimately utilized by the organism to generate energy via fermentation to ethanol, acetic acid, lactic acid, hydrogen, and/or carbon dioxide . From a bioethanol perspective, the generation of lactate, formate, and acetate remain undesirable as these competing metabolic pathways divert carbon flux away from ethanol and create a less hospitable environment when the organism is confined to culture/industrial fermenters. Although recent efforts to maximize ethanol yield in C. thermocellum by knocking out competing pathways has substantially increased the titer of ethanol produced,  much of the work-to-date focused on optimizing cellulose conversion to ethanol with model substrates, such as cellobiose, filter paper, and/or Avicel [12–15]. Thus, studies examining bacterial growth on more complex, recalcitrant, lignocellulosic material are essential, especially as the deconstruction of natural biomass is known to generate numerous antimicrobial and/or phenolic inhibitors that could ultimately impede the industrial process [16, 17].
There are few studies to date investigating C. thermocellum’s systematic response to the growth on bioenergy-relevant, lignocellulosic biomass such as pretreated switchgrass and/or Populus [18, 19]. These studies provided important clues as to how C. thermocellum deconstructs lignocellulosic biomass, but focused solely on gene expression and did not examine growth-dependent protein machinery nor the accumulation of important metabolites that could better inform the highly coordinated enzymatic process. To this end, we sought to formulate a more comprehensive, systems biology view of the deconstruction and conversion of switchgrass to ethanol by C. thermocellum over the course of batch fermentation. By integrating data obtained from three omic platforms—LC–MS/MS-based shotgun proteomics, microarray-based transcription profiling, and GC–MS-based metabolomics—we were detailed the mechanisms by which C. thermocellum adapts to the adverse environment created during lignocellulosic deconstruction, namely the release of switchgrass-derived compounds inhibitory toward growth.
To our knowledge, this is the first integrated omics interrogation of C. thermocellum’s deconstruction of a bioenergy-relevant feedstock. As the organism converts released sugars to a myriad of products, it must avoid and/or ameliorate the effects of both product-inhibition and biomass-derived cytotoxic metabolites. This information will be vitally important to metabolic engineering efforts that aim to enhance the industrial viability of bioethanol and other specialty biofuels/bioproducts.
Cultivation and sampling
Inoculum and triplicate fermentations of C. thermocellum ATCC 27405 were performed in 5-l Twin BIOSTAT® B fermenters (Sartorius Stedim North America, Bohemia, NY), as previously described, except that all vessels contained 10 g/l (dry weight basis) dilute acid pretreated switchgrass as the main substrate . Switchgrass from 4-year-old plants was pretreated with dilute sulfuric acid at the National Renewable Energy Laboratory (NREL, Golden, Colorado, USA), as previously described , washed several times with deionized water to remove soluble sugars, and dried overnight at 45 °C. MTC media were sparged overnight with nitrogen (to insure that the system was anaerobic and ready for C. thermocellum growth) before inoculation (10% v/v inoculum) to a final volume of 4 l, and the growth temperature was maintained at 58 °C . The pH was controlled at 7.0 in the fermenters with 3 N NaOH (see Additional file 1: Text S1 for additional details on fermentation). Samples were collected for metabolomics and proteomics at 19-, 43-, 91-, and 187-h postinoculation. Samples for transcriptomics were collected at 19 and 43 h. Microarray data and platform details have been deposited in the NCBI Gene Expression Omnibus (GEO) database under accession number GSE26926, with data used in this study having accession numbers GSM663002-GSM663007.
Clostridium thermocellum switchgrass fermentation samples were measured at 19, 43, 91, and 187 h, as matched for proteomic samples. Frozen cell pellets containing both microbe and plant material were weighed into 50 ml centrifuge tubes containing 10 ml of 80% ethanol, and 50 µl sorbitol (0.01000 g/ml) added as an internal standard. Samples were sonicated for 5 min (30 s on, 30 s off with an amplitude of 30%) and kept on ice. Samples were then centrifuged at 4500 rpm for 20 min, and the supernatant was decanted into scintillation vials and stored at −20 °C. One milliliter per sample was dried down, dissolved in 0.5 ml acetonitrile, and silylated to generate trimethylsilyl derivatives, as described elsewhere . After 2 days, 1 µl aliquots were injected into an Agilent 5975C inert XL gas chromatograph–mass spectrometer (GC–MS). The standard quadrupole GC–MS was operated in the electron impact (70 eV) ionization mode, targeting 2.5 full-spectrum (50–650 Da) scans per second, as described previously  (see Additional file 1: Text S1 for additional details regarding metabolites quantification). Metabolite data were expressed as fold change relative to the 19-h sampling time point with significant differences determined with Student’s t tests. Significant differences in fold change between sampling time points were also assessed with Student’s t tests.
50 ml fermentation samples were centrifuged at 8000 rpm for 20 min in a Sorvall Legend RT centrifuge (ThermoScientific, Waltham, MA, USA), and the supernatant was decanted, leaving a 5 ml pellet comprised SWG biomass, C. thermocellum, and substrate-bound cellulosomes, all of which were flash frozen in liquid nitrogen. The composite pellet was resuspended in 6 M guanidine-HCl, 10 mM DTT, and pH 8.0, and cells were then lysed by sonic disruption (Branson Ultrasonics Corp., Danbury, CT, USA). The resulting crude lysates were processed for trypsin-based bottom-up proteomics, as described in Additional file 1: Text S1. One hundred micrograms of tryptic peptides loaded onto a MudPIT column and analyzed over the course of 24-h via data dependent acquisition on a LTQ-XL mass spectrometer (Thermo Scientific). See Additional file 1: Text S1 for specific details regarding the LC separation and mapping of MS/MS spectra to predicted C. thermocellum peptides.
Prior to semi-quantitative analysis, spectral counts were rebalanced to properly distribute non-unique/shared peptides between their potential parent proteins, as previously described , and raw SpC values were converted to normalized spectral abundance factors (NSAF)  to assess quantitative differences between time points. The normalized counts of individual proteins were statistically evaluated across different time points of growth using p values derived from one-way ANOVA. Proteins with p values <0.05 were used for further clustering analysis. C-mean clustering of the proteins through Mfuzz  package was performed to identify common temporal responses. Functional enrichment (COG, KEGG, and GO) of individual cluster were performed using Fisher’s exact test through PIANO  package to generate a subnetwork of functional terms based on overlap members using a p value <0.01 in Cytoscape version 3.2.1. C. thermocellum metabolic pathways information were obtained from the KEGG pathway database . Transcriptomics measurements (19 and 43 h) and prediction of highly expressed regions of the DNA were integrated with the proteomics data and presented as a genome atlas (Fig. 2). The details of the transcriptomic measurements, as well as prediction of highly expressed genes using ‘positional preference,’ are described in Additional file 1: Text S1. All raw mass spectra for the proteome measurements have been deposited into the ProteomeXchange repository with the following accession numbers: ProteomeXchange: PXD004905; MassIVE: MSV000080128) and is available at ftp://MSV000080128@massive.ucsd.edu [password: swgtc (for reviewer access)].
Results and discussion
Microbial growth characteristics on switchgrass
Acetic acid and ethanol were the major fermentation products for wild-type C. thermocellum grown on pretreated switchgrass (Fig. 1). C. thermocellum derives ATP coupled with the production of acetic acid and ethanol. Thus, both are indicative of metabolic activity and cell growth. However, acetic acid can also be generated through deacetylation of hemicelluloses,  and thus the relative contributions from the pretreated biomass or bacteria cannot be easily differentiated in this study. Ethanol production, however, is exclusive to C. thermocellum and therefore a more specific indicator of microbial growth, especially given the complex culture conditions. The trends for acetic acid and ethanol were similar until 120-h postinoculation. Although the concentration of acetic acid was found to be 0.6 g/l after 139 h of microbial growth, ethanol stopped accumulating after 120 h. Acetic acid increased from ≤0.075 g/l at time zero to ~0.7 g/l after 187 h, and it continued to slowly accumulate throughout the experiment, consistent with other studies . The time points of 19 and 43 h represent early and late exponential growth phase, respectively, and the cultures began to transition into stationary phase around 100-h postinoculation. Earlier fermentations with this wild-type strain and substrate produced 0.5 and 0.2 g/l of acetic acid and ethanol, respectively, after 37 h . As final ethanol and acetic acid concentrations are low, the observed metabolic adaptations described below are likely in response to the chronic accumulation of other switchgrass-derived cytotoxic compounds that impact overall organismal growth.
Metabolomic analysis indicates accumulation of key metabolites that may be inhibitory to C. thermocellum growth on lignocellulosic biomass
Metabolomic analysis provided the basis for a broad analysis of metabolites resulting from C. thermocellum’s deconstruction of growth on complex lignocellulosic biomass and revealed the accumulation of metabolites derived from hemicellulose, including xylobiose, xylose, xylitol, arabinose, and arabitol (Additional file 2: Table S1). Although dilute acid pretreatment is expected to remove much of the hemicellulose, especially xylan polymers,  metabolomic identification of depolymerized xylan products suggests that a measurable portion remains even after extensive washing. This is substantiated by the continued accumulation of metabolites such as xylose and xylobiose over time, which are indicative of continued enzymatic hydrolysis. Metabolite responses are presented as the ratios of metabolite concentration at different time points (43, 91, and 187 h) relative to 19 h, the first sampling point. Of these metabolites, xylitol exhibited the largest accumulation (~9-fold) as the culture reached stationary phase. Other 5-carbon sugar alcohols, such as arabitol and ribitol, and phenolic acids derived from lignin, including caffeic acid, ferulic acid and p-coumaric acid also accumulated. A previous study of lignocellulosic hydrolysates found that many of the hemicellulose and lignin-derived components are inhibitory toward microbial growth . Thus, one must consider these cytotoxic compounds, since sugar alcohols, such as arabitol, have been found to be inhibitory to other microbes, such as T. accharolyticum (Raman B., Tschaplinski T.J., personal communication). This is especially relevant as wild-type C. thermocellum strains do not utilize hemicellulose-derived sugars.
In addition to biomass-derived metabolites, microbial-based fatty acid synthesis was evident throughout the course of the experiment. Isopalmitic acid (C16), isostearic acid (C18), and palmityl palmitic acid (C32) increased by ~15-fold by stationary phase. Other odd-carbon fatty acids, such as hentriancontanoic acid (C31H62O2), nonacosanoic acid (C29H58O2), heptacosanoic acid (C27H54O), and the methyl-branched iso-heptadecanoic acid (iso-C17H34O2), exhibited similar accumulation patterns, the latter of which increased by almost 21-fold. Since these are higher order and have odd numbers of carbon atoms, they are likely of microbial origin . The accumulation of these long-chain, saturated fatty acids, and unique branched fatty acids, indicates cell membrane restructuring to better tolerate increased concentrations of lignocellulose-derived inhibitors that affect membrane fluidity [31–33].
These data indicate that the growth of C. thermocellum on switchgrass is a complicated process whereby plant-derived glucans, which are not limiting here (Additional file 2: Table S1), fuel microbial growth, and further drive biomass deconstruction, invariably leading to the accumulation of inhibitory metabolites that negatively impact microbial metabolism. Without the active removal/bioconversion of these inhibitory compounds and/or increased tolerance, the potential application of this cellulolytic organism for effective biofuel production will be limited, especially considering the planned industrial use of complex lignocellulosic substrates like switchgrass. Fortunately, studies have shown that it is possible to generate C. thermocellum strains with improved tolerance to complex lignocellulosic substrates, such as Populus hydrolysates .
Proteomic and transcriptomic analyses reveal details of switchgrass-induced gene regulation
We extended our analyses to examine gene expression as it relates to the temporal dynamics of the proteome to better understand how C. thermocellum metabolism changes during growth and deconstructive/fermentative inhibition on switchgrass. Overall, 1551 unique proteins were identified in this study, representing ~50% of the predicted proteins listed in the C. thermocellum 27405 genome (Additional file 3: Table S2), in accord with other proteome measurements of C. thermocellum grown on Avicel or cellobiose [12, 35]. This provided reasonable coverage of key C. thermocellum systems (Additional file 4: Table S3) and allowed for detailed quantitative comparisons across the distinct lag/early exponential, late exponential and stationary growth phases to identify major proteins and pathways involved both the bioconversion of cellodextrins to energy and ethanol as well as the organism’s response to lignocellulosic metabolites released over the course of switchgrass deconstruction.
From a metabolic engineering standpoint, understanding the relationship between gene transcription (mRNA) and translation (protein) is valuable . However, insufficient RNA quality was achieved for later two time points (93 and 187 h) that precluded microarray measurements for those two samples. Nevertheless, RNA measurements from two early time points (19 and 43 h) were used to compare to similar temporal proteome measurements. Since these two transcriptomics time points correspond to early and late exponential phase, respectively, these likely reflect the most significant physiological changes in the most active growth stages. With regard to gene expression, transcriptomic measurements revealed 435 genes with varied expression (p < 0.05; fold change > or < 2.0). Among these differentially expressed genes, 38 were significantly up-regulated, while 397 were significantly down-regulated (Additional file 5: Table S4) between the time points 19 and 43 h. From a fundamental standpoint, it is possible to predict regions in the chromosome that are likely to contain genes that can be highly expressed, based on chromatin accessibility [37, 38]. Analysis of the C. thermocellum ATCC 27405 genome revealed 29 such regions along the chromosome, as indicated by dark green regions in the ‘position preference’ lane in Fig. 2. We identified 84 genes located in these regions that are less likely to be condensed by chromatin proteins and thus should be highly expressed under the appropriate conditions. Among these 84 genes, 58 (~68%) proteins expressed from these genes were detected by mass spectrometry. The comparison table of position preference, gene expression, and MS detected proteins is given in Additional file 6: Table S5. This information is a useful resource for identifying promoter sequences, open and silent regions of the genome for targeted genetics/metabolic engineering, and importantly, identification of novel metabolic features important for growth on industrially relevant substrates.
In general, the correlations of transcripts with proteins in the C. thermocellum genome are illustrated with the red/green darkly shaded regions in the outer circles. There are six regions along the chromosome (marked A–F in Fig. 2) that contain the top set of expressed genes and their abundant proteins (35 proteins see Additional file 6: Table S5). Region B encodes CelK (Cthe_0412) and CbhA (Cthe_0413), which belong to glycoside hydrolase family 9 and are both involved in polysaccharide catabolic processing. Region F encodes CipA (Cthe_3077), OlpB (Cthe_3078), and cell surface glycoprotein 2 (Orf2p; Cthe_3079), which are essential, non-catalytic cellulosome anchoring proteins. Regions A, C, D, and E encode various protein products, including NADH dehydrogenase, ferredoxin, S-layer homology domain, ribosomal proteins, DNA-directed RNA-polymerase beta subunit, and adenylate kinases . These regions are likely highly expressed under most growth conditions, but obviously can be regulated under specific growth conditions. This is exemplified by regions predicted to be highly expressed, but were detected at lower abundance (protein) or expression (mRNA) via mass spectrometry or microarray, respectively (Additional file 6: Table S5). These may be growth condition-dependent factors that are not employed for switchgrass solubilization.
Clustering of protein abundance highlights major temporal trend patterns and their overall functional signatures
Using the proteome data for relative quantitation, we sought to investigate the temporal abundance patterns of proteins during microbial growth on switchgrass. One-way ANOVA revealed 566 proteins that were significantly time dependent (p < 0.05; Additional file 7: Table S6), with each falling into one of four clusters based on the similarities between their temporal abundance profiles (Additional file 8: Fig. S1a–d). Proteins belonging to cluster 1 show a gradual decrease until late exponential phase (91 h), followed by a sharp decrease in protein abundance toward the late-stationary phase. Cluster 2 represents proteins that gradually decrease in abundance throughout the entire fermentation process. Cluster 3 represents proteins that generally increase over time, and cluster 4 represents proteins that are growth phase dependent, i.e., those that remain relatively unchanged between exponential phase time points (19 and 43 h) with a sharp increase at early log (91 h) followed by an almost negligible increase toward the end of culture (187 h).
In order to examine differential protein expression at a broad functional level, proteins within each cluster were further classified based on their COG classification, KEGG pathways, and GO categories. Functional enrichment (Fisher’s exact test) was performed on all known annotations to identify statistically significant (p < 0.01) under- or over-represented functional categories. Cluster 1 is comprised primarily of ribosomal proteins, translation-related proteins, and proteins involved in ribosome biogenesis (Additional file 8: Fig. S1a)—processes related to overall microbial growth. Thus, the precipitous fall in abundance toward late-stationary would be expected due to overall cessation of growth and metabolic activity. Cluster 2 contains some ribosome-related proteins, proteins involved in 2-oxocarboxylic acid metabolism, biosynthesis of amino acids (valine, leucine, and isoleucine), glycolysis, and TCA cycle (Additional file 8: Fig. S1b). Functional enrichment of cluster 3 shows evidence for endo-1,4-beta-xylanase activity, carbohydrate transport and metabolism, and polysaccharide catabolic processes (Additional file 8: Fig. S1c), while cluster 4 represents cellulose catabolism, post translational modification, cell division, S-layer, bacterial secretion system, ATP binding, protein export, and intracellular trafficking and secretion (Additional file 8: Fig. S1d). The predominant members of cluster 3 and cluster 4, which both generally show increased protein abundance over time, are cellulosomal proteins, which will be discussed in detail below.
Cellulosomal and other carbohydrate-active enzymes initiate cellulose deconstruction and solubilization
The first step toward switchgrass utilization by C. thermocellum involves solubilization of the complex lignocellulosic material by the organism’s cellulosome, and to a lesser extent its free enzyme systems . Cellulosomes are large, extracellular protein complexes, comprised a multitude of enzymes tethered to the cell surface through scaffold-like anchoring proteins . Overall, cellulosomal proteins exhibited increased abundance over time, with 30 of 32 significantly changing cellulosome components belonging to clusters 3 or 4 (Table 1). Four structural proteins called scaffoldins (CipA, OlpB, Orf2p, and OlpA) were among the most abundant of all detected cellulosomal proteins and were exclusive to cluster 4, which is consistent with other studies . Interestingly, two other scaffoldins, SbdA and OlpC, decreased significantly with time (cluster 2). Aside from CipA, the main enzymatic scaffold of the cellulosome, the above listed proteins are cell surface associated and thus involved in anchoring cellulosomal components to C. thermocellum. OlpA and OlpC essentially bind one catalytic enzyme each, while the other three bind CipA. CipA itself contains nine type I cohesin domains that are populated by a myriad of interchangeable, catalytic CAZymes. SdbA, Orf2p, and OlpB bind 1, 2, or 7 CipA scaffolds, respectively, leading to multiplicative increases in the number of catalytic enzymes decorating C. thermocellum (i.e., 9, 18, or 63 catalytic enzymes, respectively) . Of the three, only SdbA (1 CipA, 9 enzymes) decreases over time, indicating the decreased importance of this cell surface associate protein as the culture progresses. This is especially relevant as the other two can be “loaded” with relatively more catalytic enzyme, suggesting enhanced rates of local cellulose hydrolysis may be required as the culture progresses, perhaps as C. thermocellum begins to colonize the solid switchgrass substrate.
Among the 19 catalytic cellulosomal proteins in cluster 3 and 4, CelS, CelK, CtMan5A and CbhA were found to be most abundant and all classified under cluster 4. These enzymes provide a mixture of endo- and exo-glucanase activities to effectively access and process cellulose to smaller cellodextrins that can be imported and fermented [10, 40] and have been observed previously for cells using pure/semi-pure cellulose as the substrate [4, 15]. With regard to switchgrass-derived hemicellulose, the major constituent is xylan: a polymer of the C5 sugar xylose/arabinose. It is thus of interest to monitor the level of expressed xylanases over time. This is particularly relevant to industrial processes that would employ C. thermocellum, since the wild-type organism is unable to utilize/ferment xylans and/or C5 sugars, but requires their deconstruction for overall cellulose access [41, 42]. Note that hemicelluloses can also be inhibitory to cellulases, so it appears that there is a temporal balance between C5 and C6 deconstruction dynamics. Out of six detected xylanases, four β-xylanases (Cthe_0912, Cthe_1963, Cthe_2590, Cthe_2972) were classified under cluster 3 while Cthe_1398 (Xyloglucanase, Xgh74A) was classified under cluster 4. As mentioned above, both categories generally exhibit their highest abundance in stationary phase. The other xylanase, Cthe_1838, responsible for endohydrolysis of (1→4)-β-d-xylosidic linkages in xylans, did not change significantly over time. Considering the intertwined, heterogenous nature of lignocellulosic biomass, these xylanases play an important role in the general deconstruction of xylan-comprised hemicellulose encasing cellulose microfibrils, thus increasing the cellulase accessibility to the embedded cellulose [41, 42].
The general partitioning of xylanases to cluster 3 relative to other major cellulolytic components of the cellulosome (i.e., CipA, higher-order CipA-binding scaffoldins, and cellulases) to cluster 4 is particularly interesting. Xylanases other than Xgh74A rapidly accumulate from the start of the fermentation and increase consistently until stationary phase, whereas the cellulosome in general increases only modestly during log phase. This suggests an initial and immediate need for xylanase to deconstruct the hemicellulosic component of switchgrass and is perhaps critical to the initiation of switchgrass deconstruction.
Inspection of key metabolic pathways for hydrolyzed cellulose utilization
Clostridium thermocellum is known to utilize ABC transporter systems for active uptake of oligosaccharides generated during cellulose hydrolysis . Out of seven detected cellodextrin ABC transporter components, three (Cthe_1862, Cthe_1020, and Cthe_1019) were classified into cluster 4, whereas Cthe_0393 was a member of cluster 3. This suggests the majority of proteins required for the cellodextrin uptake remain relatively constant, with a modest increase at the transition between growth phases, similar to what was observed for CipA and other major cellulosome components. A transcriptomic analysis of C. thermocellum grown on pure cellulose showed similar findings with regard to cellodextrin transport . As shown in Fig. 3 (Additional file 9: Fig. S2), the transported cellodextrins are further processed to glucose-1-phosphate and glucose in presence of cellodextrin phosphorylase, Cthe_2989, a member of cluster 4. Glucose-1-phosphate is further converted to the glycolytic intermediate glucose-6-phosphate in the presence of phosphoglucomutase (Cthe_1265), whereas glucose converts to glucose-6-phosphate in presence of glucokinase, Cthe_0390, a member of cluster 4.
When the abundances of enzymes involved in the glycolytic pathway were assessed (Fig. 3), those involved in the conversion of glucose-6-phosphate to phosphoenol pyruvate (PEP) either had no change in expression (blue arrows) or showed decreased abundance over time (green arrows). This would be expected as the organism enters stationary phase and corroborates other studies . Although glycolytic pathway enzyme abundance is stable/reduced, there are several metabolic outcomes that are enhanced as cells leave exponential growth, namely the interconversion of oxaloacetate ⇔ malate (malate shunt) and the production of ethanol and organic acids of anaerobic fermentation. These processes are discussed below.
While several studies [35, 44] have suggested that pyruvate phosphate dikinase (PPDK; Cthe_1308) does not play a crucial role for the generation of pyruvate directly from phosphoenolpyruvate (PEP), our study finds a high abundance of PPDK that was constant throughout the time course (Fig. 3). However, C. thermocellum normally forms pyruvate via a malate shunt, which includes PEP carboxykinase (Cthe_2874; cluster 2), malate dehydrogenase (Cthe_0345; cluster 4), and malic enzyme (Cthe_0344; no change) . It has been suggested that the organism diverts carbon flow through the shunt to increase the production of the biosynthetic intermediates NADPH and GTP . All three enzymes were detected at high abundance, but had varied abundance trends.
The resulting pyruvate is the precursor for all fermentative endpoints. Examination of protein abundances profiles revealed that, except for acetate, all other end product-related enzymes appeared to increase over time, supporting the observed accumulation of these end products via microbial metabolism. These include lactate dehydrogenase (Cthe_1053; cluster 3) catalyzing pyruvate → lactate, pyruvate formate lyase (Cthe_0505 and Cthe_0506; cluster 4) catalyzing pyruvate → formate, and AdhE (Cthe_0423; cluster 4), a bidirectional acetaldehyde/CoA dehydrogenase. This enzyme functions as both an alcohol dehydrogenase and acetaldehyde dehydrogenase and is the major driver of ethanol production in C. thermocellum and other ethanologenic microbes [14, 45, 46]. These results suggest that fermentation continues as long as there is available glucose and even into stationary phase, which is in line with other studies .
Induction of pentose phosphate pathway towards late-stage culture
Even though glycolysis is the primary metabolic pathway employed by anaerobes for energy generation, we investigated how the organism may shift glycolytic intermediates to alternate pathways. This might be a useful adaptation to overcome metabolic inhibition. An example of this was observed for the pentose phosphate pathway (PPP), which utilizes glycolytic intermediates to produce pentose (C5) sugars required for the biosynthesis of nucleotides and aromatic amino acids . Out of 14 total detected PPP-related enzymes, six significantly changed with time (Fig. 4). Of these, transketolases (Cthe_2443, 2704) and ribose-phosphate pyrophosphokinase (Cthe_2630), members of cluster 4, were found to increase upon entry into early stationary phase. The high abundance and increasing accumulation of ribose-phosphate pyrophosphokinase suggests production of phosphoribosyl pyrophosphate (PRPP), a sugar precursor for purine and pyrimidine metabolism. As this portion of the PPP is generally up-regulated toward the end of the culture, where growth-related demand for nucleotides and aromatic amino acids is likely subsiding, diversion to this pathway could provide a vital shunt to divert carbon/electrons away from glycolysis in an attempt to balance metabolic flux.
With regard to the other C5 sugar-related enzymes, we also detected a very high abundance of alcohol dehydrogenase GroES-like protein (Cthe_0388; cluster 2). This enzyme converts D-xylulose (hemicellulose-derived sugar) into xylitol, which accumulated almost ninefold in late-stationary phase (Additional file 2: Table S1). Even though the abundance of this protein decreases significantly with time, it is still highly abundant during late-stationary phase and likely still involved in the interconversion of xylulose/xylitol. However, as C. thermocellum lacks an annotated xylose reductase, the only way xylitol can be formed biologically is by the reduction of xylulose, potentially by Cthe_0388 enzyme mentioned above. Further work is needed to confirm this additional functionality. Compared to xylitol, xylulose increased only ~2-fold by stationary phase. There are two possibilities for this accumulation; xylulose could be a direct product from hemicellulose degradation or there is an unknown phosphate transferase that converts xylulose-5-phosphate to/from xylulose. This could be of potential value to industrial fermentation, as xylitol is used as artificial sweetener and antimicrobial agent in foods [48, 49].
Branched-chain amino acid pools serve as precursors for higher-order fatty acid accumulation
Aside from its ultimate fermentation to ethanol and organic acids, metabolic outcomes of PEP/Pyruvate as an intermediate include the biosynthesis of the branched-chain amino acids (BCAA) valine, leucine, and isoleucine. All three amino acids accumulated—each rising to their maximum (or close to it) by 43 h of growth and remaining elevated, relative to 19 h (Additional file 2: Table S1). Leucine accumulation was most dramatic (~10-fold), followed by isoleucine (~5-fold), and valine (~1.5-fold). Although protein machinery involved in the conversion of pyruvate to BCAAs generally did not change or decreased over time (Fig. 5), a BCAA aminotransferase (Cther_0856) controlling the final step of valine, leucine, and isoleucine biosynthesis (or their degradation) increased significantly (cluster 4), suggesting an important role for these BCAAs or their derivatives.
One possibility is that C. thermocellum shifts its metabolism toward the biosynthesis of amino acids utilizing PEP/pyruvate as the precursor. This would be one of the several ways to rebalance metabolism. Diversion of carbon flux to amino acid production has been previously documented in C. thermocellum grown on model cellulose substrates like cellobiose  or pure cellulose . Although increased amino acid production was striking in these earlier studies, growth on pretreated switchgrass did not elicit the same robust response as observed with the model substrates. This was true for both intracellular and supernatant metabolite pools (data not shown). In fact, the production of amino acids due to overflow metabolism in late-stage cultures was relatively muted, appearing to preferentially affect only the BCAAs described above.
Considering the relative ratios of BCAAs observed here, especially when compared to other studies utilizing model cellulosic substrates, we report another possibility linked to the production of BCAAs that involves synthesis of higher-order fatty acids. Degradation of all three BCAAs, ultimately to isobutyryl-CoA (valine), isovaleryl-CoA (leucine), and 2-methyl-butyryl-CoA (isoleucine), gives rise to anteiso/odd, iso/odd, and iso/even type fatty acids . This could explain the large accumulation of these type of fatty acids revealed by metabolomic analysis. As shown in Fig. 5, the first step in BCAA degradation pathway requires the same up-regulated BCAA aminotransferase (Cthe_0856) involved in their synthesis. However, to obtain the activated, higher-order fatty acid precursors listed above, a second enzymatic step is required. To this end, a recent metabolic analysis of C. thermocellum has suggested a new functional annotation for the enzyme encoded by Cthe_0547 that would fulfill this role—a branched-chain keto acid dehydrogenase . This enzyme would be able to convert 2-oxoisovalerate (from valine), 4-methyl-2-oxopentanoate (from leucine), and 3-methyl-2-oxopentanoate (from isoleucine) to isobutyryl-CoA, isovaleryl-CoA, and 2-methyl-butyryl-CoA, respectively—each providing the necessary building blocks for the production of these higher-order fatty acids . Encouragingly, Cthe_0547 was found to significantly increase with time (cluster 3) providing enzymatic evidence for the measured metabolic accumulation of these types of fatty acids over the course of growth.
Fatty acid biosynthesis as a fermentation response
Bacterial fatty acid production primarily contributes to the formation and maintenance of their lipid membranes . Accumulation of specific types of fatty acids would provide a mechanism for C. thermocellum to adapt to either the build-up of fermentation products and/or lignocellulose-derived inhibitory compounds. However, as the level of ethanol and other fermentation products were not high enough to be considered inhibitory, the latter seems to be a more reasonable explanation. This is further substantiated by the accrual of lignocellulose-derived inhibitory compounds, including 4-hydroxybenzoic acid, vanillic acid, ferulic acid, p-coumaric acid, and vanillin (fold change range 2.2–1.8x), during C. thermocellum growth on switchgrass. As the chain length, saturation, and branching status of fatty acids generally control the fluidity/rigidity of the membrane, many bacteria adapt to inhibitors such as these by changing the fatty acid composition of their membrane lipids [32, 33].
Our results indicate that C. thermocellum increases the production of long-chain, branched iso- and anteiso-fatty acids over time, perhaps in response to the adverse effect imposed by lignocellulose-derived inhibitors and fermentation end products. The maintenance of optimal membrane fluidity/rigidity with branched-chain fatty acids depends on the availability of valine (anteiso/odd), leucine (iso/odd), and isoleucine (iso/even) . The metabolite abundance profiles for both the BCAAs and higher-order fatty acids measured here (Additional file 2: Table S1) indicate a substantial increase in the amount of iso-fatty acids relative to their anteiso-counterparts. This corroborates a previous study where Gram-positive bacterium Arthrobacter chlorophenolicus exposed to toxic concentrations of phenol, 4-chlorophenol, and 4-nitrophenol led to a decrease in the anteiso/iso ratio; a response that made a more rigid membrane to counteract the increase in fluidity brought upon by the cytotoxic phenolics .
All necessary enzymes illustrated in the KEGG pathway for the conversion of acetyl-CoA into palmitic acid (base fatty acid) were detected in the C. thermocellum (Additional file 10: Table S7). Overall, enzymes involved in fatty acid biosynthesis were either stable or decreased over time, again following general cessation of growth. Interestingly, the reductive steps in the biosynthesis of fatty acids require NADPH, a major product of malate shunt pathway shown to be up-regulated above (Fig. 3). In the context of higher-order, odd numbered fatty acids, a three-carbon precursor, propionyl-CoA, is required rather than acetyl-CoA . Formate acetyl transferase (Cthe_0505) is capable of producing propionyl-CoA from 2-oxobutanoate, a product formed from cysteine and methionine metabolism. This enzyme was found to increase over time (cluster 4). Together with the increase of higher-order, long-chain fatty acids observed by metabolomics analysis, these data suggest that although the base machinery for producing fatty acids decreases toward late-stage cultures as overall metabolic demand tapers off, enzymes responsible for branched, odd numbered fatty acid precursors are still required (and up-regulated) for overall membrane maintenance and/or continued turnover. This implies that the deconstruction of switchgrass imposes greater metabolic challenges on C. thermocellum than do model cellulose substrates, spurring the organism to adapt its cellular membrane to the increasingly hostile culture environment toward late-stage cultures.
Clostridium thermocellum is a candidate for consolidated bioprocessing of cellulosic biomass to biofuels. In order to understand the delicate balance between recalcitrant lignocellulose solubilization, end product formation, and potential microbial inhibition, we have conducted a detailed C. thermocellum time course omics analysis to characterize its growth on industrially relevant, dilute acid pretreated switchgrass. As expected, we found that most central metabolism-related enzymes decreased in abundance with growth, suggesting cessation of biogenesis-related activity towards late-stationary phase. In contrast, the level of enzymes involved in the production of fermentation end products remained high, potentially triggering the up-regulation of carbon/electrons diverting into metabolic pathways such as PPP to maintain metabolic flux. An important finding from this study was the observation that hemicellulose-derived 5C sugars/sugar alcohols and phenolic acids derived from lignin accumulated over time and likely contributed to growth inhibition, thus prompting up-regulation of BCAA/fatty acid re-composition pathways to adapt the cell to deteriorating culture conditions brought upon by continued deconstruction of switchgrass. This is further substantiated by the observed accumulation of higher-order fatty acids synthesized by C. thermocellum. In total, the time-dependent accumulation of long-chain iso- (and less so anteiso-) over linear fatty acids, as well as the corresponding up-regulation of the required biosynthetic protein machinery, suggests a protective effect that is imparted by these higher-order, long-chained fatty acids and is likely a necessary metabolic adaptation to growth on complex lignocellulosic biomass.
liquid chromatography–tandem mass spectrometry
gas chromatography mass spectrometry
multidimensional protein identification technology
linear trapping quadrupole
tandem mass spectrometry
normalized spectral abundance factors
analysis of variance
clusters of orthologous groups
Kyoto Encyclopedia of Genes and Genomes
American Type Culture Collection
mass spectrometry/mass spectrometer
branched-chain amino acids
Keshwani DR, Cheng JJ. Switchgrass for bioethanol and other value-added applications: a review. Bioresour Technol. 2009;100(4):1515–23.
Kacurakova M, Capek P, Sasinkova V, Wellner N, Ebringerova A. FT-IR study of plant cell wall model compounds: pectic polysaccharides and hemicelluloses. Carbohydr Polym. 2000;43(2):195–203.
Himmel ME, Ding S-Y, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD. Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science. 2007;315(5813):804–7.
Raman B, Pan C, Hurst GB, Rodriguez M Jr, McKeown CK, Lankford PK, Samatova NF, Mielenz JR. Impact of pretreated switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis. PLoS ONE. 2009;4(4):e5271.
Bayer EA, Shoham Y, Lamed R. Cellulose-decomposing bacteria and their enzyme systems. In: The prokaryotes. Berlin: Springer; 2006. p. 578–617.
Xu Q, Resch MG, Podkaminer K, Yang S, Baker JO, Donohoe BS, Wilson C, Klingeman DM, Olson DG, Decker SR. Dramatic performance of Clostridium thermocellum explained by its wide range of cellulase modalities. Sci Adv. 2016;2(2):e1501254.
Blumer-Schuette SE, Brown SD, Sander KB, Bayer EA, Kataeva I, Zurawski JV, Conway JM, Adams MW, Kelly RM. Thermophilic lignocellulose deconstruction. FEMS Microbiol Rev. 2014;38(3):393–448.
Lynd LR, Van Zyl WH, McBride JE, Laser M. Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol. 2005;16(5):577–83.
Park BH, Karpinets TV, Syed MH, Leuze MR, Uberbacher EC. CAZymes analysis toolkit (CAT): web service for searching and analyzing carbohydrate-active enzymes in a newly sequenced organism using CAZy database. Glycobiology. 2010;20(12):1574–84.
Demain AL, Newcomb M, Wu JHD. Cellulase, clostridia, and ethanol. Microbiol Mol Biol Rev. 2005;69(1):124–54.
Papanek B, Biswas R, Rydzak T, Guss AM. Elimination of metabolic pathways to all traditional fermentation products increases ethanol yields in Clostridium thermocellum. Metab Eng. 2015;32:49–54.
Yang S, Giannone RJ, Dice L, Yang ZK, Engle NL, Tschaplinski TJ, Hettich RL, Brown SD. Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress. BMC Genom. 2012;13(1):336.
Raman B, McKeown CK, Rodriguez M, Brown SD, Mielenz JR. Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation. BMC Microbiol. 2011;11(1):134.
Riederer A, Takasuka TE, Makino S-I, Stevenson DM, Bukhman YV, Elsen NL, Fox BG. Global gene expression patterns in Clostridium thermocellum as determined by microarray analysis of chemostat cultures on cellulose or cellobiose. Appl Environ Microbiol. 2011;77(4):1243–53.
Gold ND, Martin VJJ. Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis. J Bacteriol. 2007;189(19):6787–95.
Mussatto SI, Roberto IC. Alternatives for detoxification of diluted-acid lignocellulosic hydrolyzates for use in fermentative processes: a review. Bioresour Technol. 2004;93(1):1–10.
Larsson S, Reimann A, Nilvebrant N-O, Jönsson LJ. Comparison of different methods for the detoxification of lignocellulose hydrolyzates of spruce. Appl Biochem Biotechnol. 1999;77(1–3):91–103.
Wilson CM, Rodriguez CM, Johnson SL, Martin T, Chu RD, Wolfinger LJ, Hauser ML, Land DM, Klingeman MH, Syed AJ, et al. Global transcriptome analysis of Clostridium thermocellum ATCC 27405 during growth on dilute acid pretreated Populus and switchgrass. Biotechnol Biofuels. 2013;6(1):179.
Wei H, Fu Y, Magnusson L, Baker JO, Maness P-C, Xu Q, Yang S, Bowersox A, Bogorad I, Wang W. Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA-Seq. Front Microbiol. 2014;5:142.
Kridelbaugh DM, Nelson J, Engle NL, Tschaplinski TJ, Graham DE. Nitrogen and sulfur requirements for Clostridium thermocellum and Caldicellulosiruptor bescii on cellulosic substrates in minimal nutrient media. Bioresour Technol. 2013;130:125–35.
Tschaplinski TJ, Standaert RF, Engle NL, Martin MZ, Sangha AK, Parks JM, Smith JC, Samuel R, Jiang N, Pu Y. Down-regulation of the caffeic acid O-methyltransferase gene in switchgrass reveals a novel monolignol analog. Biotechnol Biofuels. 2012;5:71.
Tabb DL, Narasimhan C, Strader MB, Hettich RL. DBDigger: reorganized proteomic database identification that improves flexibility and speed. Anal Chem. 2005;77(8):2464–74.
Florens L, Carozza MJ, Swanson SK, Fournier M, Coleman MK, Workman JL, Washburn MP. Analyzing chromatin remodeling complexes using shotgun proteomics and normalized spectral abundance factors. Methods. 2006;40(4):303–11.
Kumar L, Futschik ME. Mfuzz: a software package for soft clustering of microarray data. Bioinformation. 2007;2(1):5.
Väremo L, Nookaew I. Platform for integrative analysis of omics data. 2013.
Kanehisa M, Goto S. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000;28(1):27–30.
Dumitrache A, Akinosho H, Rodriguez M, Meng X, Yoo CG, Natzke J, Engle NL, Sykes RW, Tschaplinski TJ, Muchero W. Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure. Biotechnol Biofuels. 2016;9(1):1.
Shekiro J, Kuhn EM, Selig MJ, Nagle NJ, Decker SR, Elander RT. Enzymatic conversion of xylan residues from dilute acid-pretreated corn stover. Appl Biochem Biotechnol. 2012;168(2):421–33.
Currie DH, Raman B, Gowen CM, Tschaplinski TJ, Land ML, Brown SD, Covalla SF, Klingeman DM, Yang ZK, Engle NL. Genome-scale resources for Thermoanaerobacterium saccharolyticum. BMC Syst Biol. 2015;9(1):1.
Ktsoyan ZA, Beloborodova NV, Sedrakyan AM, Osipov GA, Khachatryan ZA, Kelly D, Manukyan GP, Arakelova KA, Hovhannisyan AI, Olenin AY. Profiles of microbial fatty acids in the human metabolome are disease-specific. Front Microbiol. 2010;1:148.
Timmons MD, Knutson BL, Nokes SE, Strobel HJ, Lynn BC. Analysis of composition and structure of Clostridium thermocellum membranes from wild-type and ethanol-adapted strains. Appl Microbiol Biotechnol. 2009;82(5):929–39.
Ibraheem O, Ndimba BK. Molecular adaptation mechanisms employed by ethanologenic bacteria in response to lignocellulose-derived inhibitory compounds. Int J Biol Sci. 2013;9(6):598.
Murínová S, Dercová K. Response mechanisms of bacterial degraders to environmental contaminants on the level of cell walls and cytoplasmic membrane. Int J Microbiol. 2014;2014:16.
Linville JL, Rodriguez M Jr, Land M, Syed MH, Engle NL, Tschaplinski TJ, Mielenz JR, Cox CD. Industrial robustness: understanding the mechanism of tolerance for the Populus hydrolysate-tolerant mutant strain of Clostridium thermocellum. PLoS ONE. 2013;8(10):e78829.
Burton E, Martin VJJ. Proteomic analysis of Clostridium thermocellum ATCC 27405 reveals the upregulation of an alternative transhydrogenase–malate pathway and nitrogen assimilation in cells grown on cellulose. Can J Microbiol. 2012;58(12):1378–88.
Martín J-F, Liras P. Engineering of regulatory cascades and networks controlling antibiotic biosynthesis in Streptomyces. Curr Opin Microbiol. 2010;13(3):263–73.
Pedersen AG, Jensen LJ, Brunak S, Stærfeldt H-H, Ussery DW. A DNA structural atlas for Escherichia coli. J Mol Biol. 2000;299(4):907–30.
Satchwell SC, Drew HR, Travers AA. Sequence periodicities in chicken nucleosome core DNA. J Mol Biol. 1986;191(4):659–75.
Bayer EA, Chanzy H, Lamed R, Shoham Y. Cellulose, cellulases and cellulosomes. Curr Opin Struct Biol. 1998;8(5):548–57.
Zhang Y-HP, Lynd LR. Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation. Proc Natl Acad Sci USA. 2005;102(20):7321–5.
Dürre P. Handbook on clostridia. Boca Raton: CRC Press; 2005.
Dror TW, Rolider A, Bayer EA, Lamed R, Shoham Y. Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase. J Bacteriol. 2005;187(7):2261–6.
Strobel H, Caldwell F, Dawson K. Carbohydrate transport by the anaerobic thermophile Clostridium thermocellum LQRI. Appl Environ Microbiol. 1995;61(11):4012–5.
Zhou J, Olson DG, Argyros DA, Deng Y, van Gulik WM, van Dijken JP, Lynd LR. Atypical glycolysis in Clostridium thermocellum. Appl Environ Microbiol. 2013;79(9):3000–8.
Lo J, Zheng T, Hon S, Olson DG, Lynd LR. The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum. J Bacteriol. 2015;197(8):1386–93.
Brown SD, Guss AM, Karpinets TV, Parks JM, Smolin N, Yang S, Land ML, Klingeman DM, Bhandiwad A, Rodriguez M. Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum. Proc Natl Acad Sci. 2011;108(33):13752–7.
Holwerda EK, Thorne PG, Olson DG, Amador-Noguez D, Engle NL, Tschaplinski TJ, van Dijken JP, Lynd LR. The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading. Biotechnol Biofuels. 2014;7(1):155.
Granström TB, Izumori K, Leisola M. A rare sugar xylitol. Part II: biotechnological production and future applications of xylitol. Appl Microbiol Biotechnol. 2007;74(2):273–6.
Alves LA, Felipe MG, Silva JBAE, Silva SS, Prata AM. Pretreatment of sugarcane bagasse hemicellulose hydrolysate for xylitol production by Candida guilliermondii. Appl Biochem Biotechnol. 1998;70(1):89–98.
Van Der Veen D, Lo J, Brown SD, Johnson CM, Tschaplinski TJ, Martin M, Engle NL, Van den Berg RA, Argyros AD, Caiazza NC. Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways. J Ind Microbiol Biotechnol. 2013;40(7):725–34.
Klein W, Weber MH, Marahiel MA. Cold shock response of Bacillus subtilis: isoleucine-dependent switch in the fatty acid branching pattern for membrane adaptation to low temperatures. J Bacteriol. 1999;181(17):5341–9.
Roberts SB, Gowen CM, Brooks JP, Fong SS. Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production. BMC Syst Biol. 2010;4(1):1.
Cronan JE, Thomas J. Bacterial fatty acid synthesis and its relationships with polyketide synthetic pathways. Methods Enzymol. 2009;459:395–433.
Unell M, Kabelitz N, Jansson JK, Heipieper HJ. Adaptation of the psychrotroph Arthrobacter chlorophenolicus A6 to growth temperature and the presence of phenols by changes in the anteiso/iso ratio of branched fatty acids. FEMS Microbiol Lett. 2007;266(2):138–43.
Berg JM, Tymoczko JL, Stryer L. Biochemistry. In: Freeman WH, editor. Fatty acids are synthesized and degraded by different pathways, 5th edition, Section 22.4. New York: Basingstoke; 2002.
BR, JRM, SDB, and RLH designed the project and defined the overall experimental approach. BR, JRM, and MRJ conducted all cultivation and sampling for the C. thermocellum fermentations, and generated growth curve information. SP, RJG, and RLH conducted all proteome measurements and associated data analyses. MZM, NLE, and TJT conducted all metabolomic measurements and associated data analyses. SDB coordinated all the transcriptomics measurements. SP, IN, and DU conducted all bioinformatic work on correlating the transcriptomics and proteomics measurements to generate the “position preference” map. All authors contributed to manuscript preparation and editing, but this work was mainly led by SP, RJG, and RLH. All authors read and approved the final manuscript.
The authors would like to acknowledge ORNL staff members Zamin K. Yang for help with RNA extractions/QC, and Lezlee Dice for DNA microarray work. Additional acknowledgement is given to ORNL researchers Adam Guss and James Elkins for helpful technical comments during the preparation of this manuscript. This study was funded by the BioEnergy Science Center, a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Oak Ridge National Laboratory is managed by University of Tennessee-Battelle LLC for the Department of Energy under contract DOE-AC05-00OR22725.
The authors declare that they have no competing interests.
Availability of supporting data
All supplemental data (and repository information for transcriptome and proteome measurements) have been made freely available; specific data repository information are specified within the manuscript.
This study was funded by the BioEnergy Science Center, a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the US DOE Office of Science.
Suresh Poudel and Richard J. Giannone contributed equally to this work
About this article
Cite this article
Poudel, S., Giannone, R.J., Rodriguez, M. et al. Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass. Biotechnol Biofuels 10, 14 (2017). https://doi.org/10.1186/s13068-016-0697-5
- Clostridium thermocellum
- Mass spectrometry