Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance
© The Author(s) 2017
Received: 10 August 2017
Accepted: 7 November 2017
Published: 14 November 2017
The Bacillus subtilis endo-β-1,4-glucanase (BsCel5A) hydrolyzes β-1,3-1,4-linked glucan, and the enzyme includes a family 3 carbohydrate-binding module (CBM3) that binds β-1,4-linked glucan.
Here we investigate the BsCel5A β-1,3-1,4 glucanase activity after exchanging the CBM3 domain for the family 11 CBM from Ruminiclostridium thermocellum celH (RtCBM11) having β-1,3-1,4 glucan affinity.
The BsCel5A-RtCBM11 presents a 50.4% increase in Vmax, a 10% reduction in K0.5, and a 2.1-fold increase in catalytic efficiency. Enzyme mobility and binding to barley β-1,3-1,4 glucan and pre-treated sugarcane bagasse were investigated using Electron Paramagnetic Resonance (EPR) with Site-Directed Spin Labeling (SDSL) of the binding site regions of the CBM3 and RtCBM11 domains in the BsCel5A-CBM3 and BsCel5A-RtCBM11, respectively. Although higher mobility than the RtCBM11 was shown, no interaction of the spin-labeled CBM3 with β-1,3-1,4 glucan was observed. In contrast, a Ka value of 0.22 mg/mL was estimated from titration of the BsCel5A-RtCBM11 with β-1,3-1,4 glucan. Enzyme binding as inferred from altered EPR spectra of the BsCel5A-RtCBM11 was observed only after xylan or lignin extraction from sugarcane bagasse. Binding to xylan- or lignin-free lignocellulose was correlated with a 4.5- to 5-fold increase in total reducing sugar release as compared to the milled intact sugarcane bagasse, suggesting that xylan impedes enzyme access to the β-1,3-1,4 glucan.
These results show that the non-specific binding of the BsCel5A-RtCBM11 to the lignin component of the cell wall is minimal, and represent the first reported use of EPR to directly study the interaction of glycoside hydrolyse enzymes with natural insoluble substrates.
Cellulose is the major component of the plant cell wall, and is integrated with various other polysaccharides and lignin to form a complex polymer matrix. Cellulose forms linear chains comprising glucose monomers linked by β-1,4-glycosidic bonds . Abundant hydrogen bonding both within and between the cellulose polymers imposes a largely crystalline structure that is recalcitrant to degradation . The enzymatic hydrolysis of cellulose requires a consortium of glycoside hydrolases initiated by the action of endoglucanases (endo-1,4-β-glucosidases: EG; EC 188.8.131.52) that cleave β-1,4-glycosidic bonds in the regions of the cellulose fibers showing low crystallinity, which results in the release of oligosaccharides, cellobiose, and glucose. Subsequently, exoglucanases or cellobiohydrolases (exo-1,4-β-glucosidases; CBH; EC 184.108.40.206) act on the reducing and non-reducing extremities in crystalline regions of the cellulose fibers to release glucose and cellobiose, and finally β-glucosidases hydrolyze short cell oligosaccharides and cellobiose to release glucose . This synergistic hydrolysis of cellulose contributes to carbon recycling in the biosphere [3–5], and has been extensively studied from the viewpoint of biotechnological applications in the biofuels, animal feed, pulp bleaching, wastewater treatment, and paper bioconversion industries [6, 7]. The use of enzymes can reduce energy costs and avoid the use of chemical processes that could ultimately harm the natural environment .
Endo- and exoglucanases frequently comprise two or more domains that display multi-functional properties, where a catalytic domain (CD) is responsible for the hydrolytic activity, and non-catalytic domains enhance catalysis or perform structural functions . The non-catalytic modules include the Carbohydrate-Binding Modules (CBMs) , which are coupled to the catalytic domains via flexible polypeptide linkers . It is widely recognized that the CBM domains of cellulases improve the efficiency of enzymatic hydrolysis of insoluble substrates  as a result of their functions in polysaccharide recognition and binding . The CBMs are currently grouped into 81 families (CAZy database, http://www.cazy.org) that display variable binding specificities against different plant cell wall polysaccharides such as crystalline or amorphous cellulose, β-glucans, xylans, laminarins, mannans, galactans, xyloglucans, arabinans, and others.
Electron Paramagnetic Resonance (EPR) spectroscopy is a well-established technique, which targets defined paramagnetic centers  and is not subject to interference from signal scattering resulting from the presence of particulate material . EPR spectroscopy together with site-directed spin labeling (SDSL) has been used to investigate structural and conformational changes of proteins [16–18]. The use of EPR is well suited to study the binding of spin-labeled proteins to lignocellulosic biomass, and in contrast to Nuclear Magnetic Resonance (NMR) and X-ray crystallography, the technique can be applied under conditions found in natura  or under high solids loading. In SDSL, an extrinsic spin probe is covalently attached to the thiol side chain in a native or site-directed mutated Cys residue , and analyses of the EPR spectrum can provide information with respect to protein chain dynamics, solvent accessibility, local polarity, and protein interactions under the chosen experimental conditions [14, 20–24]. Furthermore, when multiple probes are introduced into a protein, EPR-SDSL can be used to measure distances between the spin probes within the polypeptide chain [25, 26].
The thermostable Bacillus subtilis cellulase 5A (BsCel5A) is a well-characterized endo-β-1,4-glucanase, and comprises a family 5 glycoside hydrolase (GH5) catalytic domain fused to a family 3 carbohydrate-binding module (CBM3) . The CBM3 family has an extended planar binding surface, and has been proposed to interact mainly with insoluble substrates such as crystalline cellulose . Biochemical characterization of BsCel5A showed a maximum enzymatic activity at pH 6 and 60 °C, and a high thermal stability . Although the BsCel5A hydrolyzes the β-1,4 linkages both in soluble carboxymethylcellulose (CMC) and in crystalline cellulose, the enzyme has a 5-fold higher catalytic efficiency against barley β-glucan and lichenan , which are soluble polysaccharides composed of β-1,3-β-1,4 mixed linked glucose monomers .
The CelH of Ruminiclostridium thermocellum (previously Clostridium thermocellum) is a multidomain protein , which encodes an N-terminal GH26 β-1,3-1,4-glucanase catalytic domain, a second catalytic GH5 β-1,4-cellulase domain, a family 11 carbohydrate-binding module (RtCBM11), and a dockerin domain at the C-terminus [29, 30]. Since the RtCBM11 domain has high affinity for β-glucan polymers, we surmised that its fusion with the catalytic domain of BsCel5A could increase the β-1,3-1,4-glucanase activity of the enzyme. Here we report the application of EPR-SDSL to investigate the dynamics and binding characteristics of the BsCel5A catalytic domain fused with the carbohydrate-binding modules CBM3 and RtCBM11 in the presence of the natural substrate barley β-glucan and several types of pre-treated sugarcane bagasse.
Cloning, expression, and purification
Expression systems in E. coli using the pET28a vector have been previously described for the Bacillus subtilis BsCel5A-CBM3  and the RtCBM11 from the celH of R. thermocellum . The RtCBM11 nucleotide sequence cloned in the pET28a plasmid was used as template for amplification with oligonucleotides CBM_Bsp_F (containing restriction site BspI) and the T7 terminator sequence (containing restriction site BamHI). The amplified fragment was purified from 1% agarose gels using the GeneJET kit (Fermentas, Vilnius, Lithuania), and digested with restriction enzymes BspI and BamHI. Digestion of the BsCel5A-CBM3_pET28a construct with the same two restriction enzymes liberates the sequence encoding the CBM3 domain, and generates compatible cohesive ends for ligating the digested RtCBM11 PCR fragment. Following ligation, the integrity of the BsCel5A-RtCBM11_pET28a construct was confirmed by automated nucleotide sequencing.
Competent E. coli Rosetta BL21 (DE3) cells were transformed with either the BsCel5A-CBM3 or the BsCel5A-RtCBM11. After regeneration, the transformed cells were cultured in 10 mL of selective LB liquid at 37 °C for 16 h. These cultures were used to inoculate 1 L of selective LB liquid medium, and after an appropriate cell density had been reached (OD600 = 0.7) protein expression was induced by the addition of 0.5 mM IPTG (isopropyl-β-d-thiogalactopyranoside) and ran for 6 h under the same culture conditions. Subsequently, the cells were harvested by centrifugation, and lysed by sonication in buffer containing 40 mM HEPES, 300 mM NaCl, pH 7.5, and 40 mM imidazole. After centrifugation to clear cell debris, the supernatant was incubated with 2 mL of Ni–NTA Superflow resin (Qiagen) in an orbital shaker at 4 °C at 50 rpm for 60 min, and packed into a suitable chromatographic column. After passing 40 mL of wash buffer (60 mM Imidazole, 100 mM HEPES, 30 mM NaCl, pH 7.5), the recombinant proteins were recovered with elution buffer (300 mM Imidazole, 100 mM HEPES, 300 mM NaCl, pH 7.5). Eluted protein samples were concentrated and further purified by gel filtration (Superdex 200, 5 × 150 mm column) equilibrated with 25 mM HEPES, 250 mM NaCl, pH 6, with a solvent flow rate of 0.5 mL/min.
Circular dichroism (CD)
Far-UV (190–250 nm) CD measurements were performed with a Jasco J-815 CD Spectrometer (JASCO Corporation, Japan) using a 1--mm path length quartz cell using protein samples at a final concentration of 2 μM in 10 mM of sodium phosphate buffer, pH 8.0. The spectra were recorded at a scan speed of 50 nm/min and at time response of 1 s. An average of 9 spectra were recorded, the buffer baseline was subtracted and after Savitzky–Golay smoothing using the CDTools software , the processed spectra were deconvoluted by the ContinII software  with database 4  available in the DichroWeb analysis server . The normalized root mean-square deviation (NRMSD) goodness-of-fit parameter was < 0.15, suggesting that the calculated spectra are a good approximation of the experimental data .
Enzymatic assays and determination of the kinetic parameters
The hydrolysis of carboxymethylcellulose (CMC, Sigma-Aldrich Chem. Co.) and barley β-glucan  was assayed at 50 °C in Bis–Tris buffer, pH 6.0, in a final volume of 0.6 mL. The reducing sugars released were quantified using the dinitrosalicylic acid (DNS) method . Controls with heat-inactivated enzyme were included in all enzymatic assays. The experimental conditions (reaction times, enzymatic units) were adjusted to guarantee the estimation of initial velocities. One enzyme unit (U) was defined as the amount of enzyme that releases 1 μmol of product per min. The specific activity was defined as the enzyme units per milligram of total protein (U mg−1). All enzymatic assays were performed in duplicate. Maximum velocities (V max) and apparent affinity constants (K 0.5) for the hydrolysis of CMC and β-glucan by BsCel5A-RtCBM11 and BsCel5A-CBM3 were estimated using the SigrafW Software . All experimental kinetic curves were repeated three times using different recombinant enzyme preparations, where each experimental point was assayed in duplicate. The kinetic parameters presented are calculated values and are given as the mean ± SD of the three replicates (n = 3).
Site-directed spin labeling and EPR measures
The purified proteins were transferred to labeling buffer (40 mM Hepes, 300 mM NaCl, pH 8.0), and immediately incubated with a 5-fold molar excess of the spin-labeled 2,5-dihydro-2,2,5,5-tetramethyl-3-[[(methylsulfonyl)thio]methyl]-1H-pyrrol-1-yloxy (MTSSL) for 24 h at 25 °C. The modified protein was separated from excess free spin label with a Superdex 200 column (5 × 150 mm) eluted with 40 mM HEPES, 300 mM NaCl, and pH 8.0 buffer. The spin-labeled proteins were concentrated to 200 μM by ultrafiltration.
Continuous wave EPR spectroscopy was performed at room temperature using a JEOL JES-FA200 equipped with temperature control and a cylindrical cavity operating in X band mode. Spectral analyses were performed according to empirical parameters as previously described (Columbus and Hubbell, 2004). The Ms mobility parameter was calculated as function of the central line width as Ms = (δ exp −1 − δ i −1)/(δ m −1 − δ i −1 ), where δ exp is the value of the central line width of R1 in the MTSSL-labeled protein. The δ i = 8.4 G and δ m = 2.1 G are the values of the greatest degree of either immobilization or mobility of sites observed in a protein, respectively, as obtained in studies with rhodopsin [14, 18].
Solutions containing labeled proteins free in solution, and in the presence of either purified β-glucan or pre-treated sugarcane cell wall samples were prepared and drawn into capillary tubes. The final protein concentration was 100 μM, and the β-glucan concentration was varied from 0.1 to 5 mg/mL. The sugarcane cell wall preparations were used at a concentration of 1 mg/mL. The titration curve was fitted to a sigmoidal Hill function by non-linear regression using the program Microcal Origin version 8.0, to yield estimates of the association constant (Ka).
Sugarcane cell wall preparation, fractionation, and enzymatic assay
Five hundred milligrams of sugarcane bagasse culms were hammer-milled to a 0.5 mm particle size, and extracted 6 times with 25 mL of 80% (v/v) ethanol at 80 °C for 20 min to remove sugars and other soluble compounds. The alcohol insoluble residue (AIR) was washed with distilled water and dried at 60 °C for 24 h . The AIR was subjected to two consecutives extractions with 25 mL of 0.5 M ammonium oxalate pH 7.0 at 80 °C for 3 h with continuous stirring. The solid oxalate-extracted residue was recovered by centrifugation at 13,000g for 30 min and dried at 60 °C for 24 h . Subsequent extraction with DMSO followed a previously reported protocol , where approximately 4 g of oxalate-extracted sugarcane bagasse was subjected to two DMSO extractions using a ratio of 14 mL DMSO per gram of biomass at room temperature with continuous stirring at 20 rpm at 70 °C for 2 h. The solids fraction containing the DMSO-extracted biomass was recovered and washed with ethanol and dried . Biomass delignification was performed as previously described  where approximately 10 g of milled sugarcane bagasse was suspended in 100 mL of water to which was added 4 g NaClO2, followed by the addition of 500 mL of glacial acetic acid. The mixture was incubated at 60 °C for 3 h. At hourly intervals, 4 g of NaClO2 was added until a total of 0.80 g of NaClO2/g of biomass was reached. The mixture was vacuum filtered and the solid fraction was washed with deionized water and dried at 50 °C for 24 h [41, 42].
The activity of the enzymes against AIR and all the treated lignocellulosic substrates was performed using a 1% w/v suspension of the treated substrate prepared in 50 mM Bis–Tris buffer, pH 6.0, buffer, 100 μM of either BsCel5A-CBM3 or BsCel5A-RtCBM11 at 50 °C for 24 h in an orbital shaker at 200 rpm. The release of total reducing sugars was measured by the DNS method .
Results and discussion
Exchanging the CBM3 domain of the BsCel5A for RtCBM11 increases catalytic efficiency
The cysteine mutants of the Bscel5A-CBM3 and Bscel5A-RtCBM11 nucleotide coding sequences encode polypeptides of 494 and 514 amino acids, respectively, and were successfully expressed as soluble proteins in E. coli Roseta (DE3). The molecular masses of the cysteine mutant proteins BsCel5A-CBM3 and BsCel5A-RtCBM11 were 54,787.9 and 56,897.3 Da, respectively, including the polyhistidine sequence added to the N-termini derived from the pET28a plasmid. The relative molecular mass of both enzymes estimated by gel filtration was approximately 55 kDa (data not shown), and it was therefore assumed that both enzymes are monomeric. The far-UV CD spectra of the pure enzymes show a maximum at approximately 195 nm, a minimum around 215 nm, and a shoulder at 225 nm (Additional file 1: Figure S1) that are characteristic of spectra from proteins with a mixed α-helix and β-strand secondary structure content. The secondary structure content (inset to Additional file 1: Figure S1) estimated by deconvolution of the CD spectra of the BsCel5A-CBM3 was 26.4% α-helix and 20.7% β-strand and for the BsCel5A-CBM3 was 26.6% α-helix and 22.3% β-strand. These values are consistent with the sum of the secondary structure assignments from the crystallographic structure of the BsCel5A catalytic domain (PDB 3PZV, ) with either the CBM3 (PDB 2L8A , sum of 22.5% α-helix and 21.5% β-strand) or the RtCBM11 (PDB 1V0A , sum of 21.2% α-helix and 27.0% β-strand).
Kinetic parameters for β-glucan and CMC hydrolysis by cysteine mutants BsCel5A-CBM3 and BsCel5A-CBM11 enzymes
V max (U mg−1)
K 0.5 (mg mL−1)
K cat (s−1)
K cat/K 0.5 (mL mg−1 s−1)
498.1 ± 20.1
2.88 ± 0.05
472.34 ± 20.1
218.7 ± 10.4
6.97 ± 0.15
199.70 ± 10.4
985.7 ± 40.2
2.66 ± 0.04
934.72 ± 40.2
251.5 ± 9.6
6.98 ± 0.22
238.49 ± 9.6
Binding of the BsCel5A-RtCBM11Y151R1 to β-glucan is confirmed by EPR-SDSL
Experimental strategies used to study polysaccharide binding to CBMs include affinity electrophoresis, insoluble polysaccharide pulldown, and NMR . Here we have introduced a novel and complementary method to investigate CBM binding dynamics based on spin labeling electron paramagnetic resonance (SDSL-EPR) experiments. We have produced single cysteine mutants in the carbohydrate-binding regions of the CBM domains in both the BsCel5A-CBM3 and BsCel5A-RtCBM11, and modified these cysteines with the spin probe MTSSL. The labeled proteins were used to analyze changes in the dynamics of both enzymes in the presence of the natural substrate β-glucan and several pre-treated biomass samples.
The CBM3 domain from BsCel5A and the RtCBM11 structures presented in Fig. 2 also highlights the residues labeled with the MTSSL probe in the spin-labeling EPR experiments. The crystal structure of the wild-type CBM3 domain from BsCel5A (PDB code 2L8A) reveals two unpaired cysteine residues (C405 and C412). Residue C412 in the BsCel5A-CBM3 was mutated to alanine resulting in a protein containing the single cysteine C405. The wild-type RtCBM11 does not include any cysteine residues, and analysis of the crystal structure (PDB code 1V0A) reveals that the Y151 is located in the substrate binding cleft, which is convenient for reporting local changes by SDSL-EPR. This residue was mutated in the BsCel5A-RtCBM11 to generate the Y151C single cysteine mutant. Both single cysteine mutant proteins were successfully labeled with the MTSSL spin probe to generate the BsCel5A-RtCBM11Y151R1 and BsCel5A-CBM3C405R1 and used in SLDL-EPR experiments.
EPR-SDSL can measure the binding affinity of BsCel5A-RtCBM11Y151R1 to β-glucan
Ms parameter estimation from EPR spectra of labeled proteins
BsCel5A-CBM3C405R1 in solution
BsCel5A-RtCBM11Y151R1 in solution
BsCel5A-RtCBM11Y151R1 + milled sugarcane
BsCel5A-RtCBM11Y151R1 1 + OA residual fraction
BsCel5A-RtCBM11Y151R1 1 + AIR fraction
BsCel5A-RtCBM11Y151R1 1 + DMSO residual fraction
BsCel5A-RtCBM11Y151R1 1 + delignificated fraction
Comparison of the titration results from EPR with the results from reducing sugar release reveals that the RtCBM11 binding is saturated at β-glucan concentrations of less than 0.5 mg/mL, whereas the measured catalytic activity of the BsCel5A-RtCBM11 continues to increase even at a β-glucan concentration of 15 mg/mL. The high-affinity association of the Type B RtCBM11 within the β-glucan might be expected to facilitate the binding of the BsCel5-RtCBM11 and enhance catalysis by the active site domain. After hydrolysis, we propose that a reduced affinity of the RtCBM11 exo-binding at the polysaccharide terminus generated by hydrolysis favors dissociation even at high β-glucan concentrations, thereby liberating the enzyme for high-affinity endo-binding to the substrate. We note that no significant change was observed in the K0.5 between the BsCel5A-CBM3 and BsCel5A-RtCBM11 constructs, further indicating that the catalytic and carbohydrate-binding domains can function independently. It has previously been observed that a xyloglucan-specific endo-β-1,4-glucanase from Aspergillus niveus (XegA) fused to a xyloglucan-specific CBM44 domain from the CelJ of R. thermocellum (RtCBM44) exhibits a 30% increase in the catalytic efficiency for xyloglucan as compared to the parental enzyme . It was proposed that the CBM in the XegA-RtCBM44 chimera alters the topology of the carbohydrate interaction in the active site of the XegA-RtCBM44 chimera thereby promoting a favorable conformation of the substrate in the catalytic domain .
Lignocellulose binding of BsCel5A-RtCBM11Y151R1 measured by EPR-SDSL correlates with reducing sugar release
The different components of the cell wall lignocellulose can be selectively extracted with alternative chemical treatments that break or rearrange the covalent and non-covalent linkages between polysaccharide structures . These treatments reduce the polysaccharide crystallinity and increase the surface area, which facilitates enzyme access resulting in enhanced hydrolysis [52, 53]). Here we have performed a hierarchical cell wall deconstruction with chemical treatments that sequentially modifies the polysaccharide and lignin content of the biomass.
The first treatment is with ethanol and water to remove the soluble sugars and other soluble components to leave the alcohol insoluble residue (AIR). The EPR spectra of the BsCel5A-RtCBM11Y151R1 in the presence of this material (Fig. 5) did not present significant differences in the Ms parameter as compared to the free protein control in solution. The biomass was subsequently extracted with ammonium oxalate to release proteins and pectin (the AO residual fraction), and consequently the resulting material has a reduced content of neutral monosaccharides and uronic acids ). The EPR spectrum of the BsCel5A-RtCBM11Y151R1 collected with the AO residual fraction shows only slight modifications (Fig. 5). The next fractionating step was performed with DMSO to yield the DMSO residual fraction. In sugarcane cell walls, the xylan polymers contain β-1,4-linked d-xylose units that are modified as O-acetyl-4-O-methylglucurono-xylan, and the removal of this polysaccharide increases the efficiency of cellulose hydrolysis [54, 55]. The DMSO treatment removes the soluble cell wall xylan [40, 56]. The EPR spectrum of the BsCel5A-RtCBM11Y151R1 with the DMSO residual fraction shows a visible increase in out-peak separation and a reduced Ms value as a result of the interaction with RtCBM11 domain with the treated biomass (Fig. 5).
The final fractionation step is the removal of lignin, which has a supramolecular polymeric structure derived from aromatic groups linked by β-O-4a alkyl-ari-ether bonds [57, 58]. The role of lignin in the plant cell wall is both structural and to protect from microbial degradation , and it has been suggested that the removal of lignin is the key to the increased hydrolysis of lignocellulosic material [58, 60–63]. The EPR spectrum of the BsCel5A-RtCBM11Y151R1 in the presence of the delignificated cell wall fraction (Fig. 5) is visibly altered, showing a decrease in the Ms parameter due to reduced labeled side chain dynamics, and provides evidence for the interaction of the protein with the treated biomass.
These results were correlated with the results of total reducing sugar release using sugarcane bagasse after the same sequence of treatments as used in the EPR experiments. Reducing sugar release (Fig. 5b) on incubation of the BsCel5A-RtCBM11 with either AIR or with the AO residual fraction shows no significant difference when compared to the untreated milled bagasse. However, after the treatments for xylan and lignin removal from the bagasse, the total reducing sugar release increases by approximately 4.5- and 5-fold, respectively (Fig. 5b). The increased hydrolysis efficiency against both the DMSO residual fraction and the delignified sugarcane bagasse is strongly correlated with the increased BsCel5A-RtCBM11Y151R1 binding observed in the EPR experiments. It has been demonstrated that non-selective binding of hydrolytic enzymes to plant cell wall lignin can reduce their catalytic activity , and it has been suggested that this binding effect may present a major obstacle to the use of enzymes for the deconstruction of lignocellulose . The majority of previous reports have estimated enzyme binding to biomass indirectly by measuring the residual catalytic activity in supernatants after incubation with biomass, whereas the present study has investigated the binding of BsCel5A-RtCBM11Y151R1 to treated sugarcane by direct measurement with EPR spectroscopy. In contrast to many previous reports, our results have demonstrated that only low levels of protein bind to samples in which lignin is present. Our results further indicate that in the case of the BsCel5A-RtCBM11, removal of the O-acetyl-4-O-methylglucurono-xylan with DMSO is the key treatment of the sugarcane resulting in both an increase in enzyme binding and a concomitant increase in reducing sugar release. The protective effect of the O-acetyl-4-O-methylglucurono-xylan suggests that the sequence of deconstruction of sugarcane cell wall requires the prior enzymatic or chemical removal of xylans for the exposure to hydrolysis by the β-glucanase. This implies that β-glucanase activity against cell wall lignocellulose would be enhanced in the presence of a xylanase, and indeed synergy between these two enzymes has been observed in the hydrolysis of wheat lignocellulose .
Here we have shown that substitution of the naturally occurring CBM3 domain in the BsCel5-CBM3 by the RtCBM11 results in an increase in the hydrolytic efficiency of the enzyme. We have also shown for the first time through direct measurements by EPR spectroscopy that the CBM3 of the BsCel5A-CBM3 does not bind to β-glucan substrate, suggesting that the CBM3 and catalytic domains may act independently. The EPR measurements further reveal that the RtCBM11 of the BsCel5A-RtCBM11 displays high affinity for β-glucan, which is correlated with the increase in catalytic efficiency of the enzyme against this polysaccharide. To fully exploit the potential of EPR spectroscopy for the study of CBM binding in a biologically relevant environment, we have analyzed the association of the BsCel5A-RtCBM11 against a series of insoluble sugarcane bagasse fractions. The results suggest that hemicellulose impedes the binding of BsCel5A-RtCBM11 and the hydrolysis of the β-glucan in the sugarcane cell wall. This demonstrates the potential of EPR spectroscopy to study the interaction of hydrolases with complex insoluble lignocellulosic materials.
RF-M designed the experiments, performed mutagenesis, protein purification and modification, and biochemical characterization, and drafted the manuscript. LPM, LFA, and SC performed the kinetic measurements and participated in the preparation of the manuscript. LFSM, LDM, and LGMB collected, processed, and analyzed the EPR data and participated in the preparation of the manuscript. AJC-F and RJW participated in experimental design, coordination of the study, and preparation and revision of the manuscript. All authors read and approved the final manuscript.
This work was funded by FAPESP Grants 2010/18850-2 (RJW), 2015/18390-5 (AJCF), 2015/18149-6 (RFM), INCT/CNPq phase II (CNPq 465319/2014-9, FAPESP 2014/50884-5), and CNPq grant 307652/2013-0 (RJW).
The authors declare that they have no competing interests.
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