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  1. Photosynthetic organisms can be used for renewable and sustainable production of fuels and high-value compounds from natural resources. Costs for design and operation of large-scale algae cultivation systems c...

    Authors: Robert J. Flassig, Melanie Fachet, Kai Höffner, Paul I. Barton and Kai Sundmacher
    Citation: Biotechnology for Biofuels 2016 9:165
  2. Biohythane is a new and high-value transportation fuel present as a mixture of biomethane and biohydrogen. It has been produced from different organic matters using anaerobic digestion. Bioenergy can be recove...

    Authors: Qian Liu, Zhiyong Jason Ren, Cong Huang, Bingfeng Liu, Nanqi Ren and Defeng Xing
    Citation: Biotechnology for Biofuels 2016 9:162
  3. As a key strategy for mitigating global climate change, bioenergy production by reducing CO2 emissions plays an important role in ensuring sustainable development. However, land-use change by converting natural e...

    Authors: Wei Liu, Cheng Peng, Zhifen Chen, Yue Liu, Juan Yan, Jianqiang Li and Tao Sang
    Citation: Biotechnology for Biofuels 2016 9:161
  4. Concerns around greenhouse gas emissions necessitate the development of sustainable processes for the production of chemicals, materials, and fuels from alternative renewable sources. The lignocellulosic plant...

    Authors: Ramakrishnan Parthasarathi, Jian Sun, Tanmoy Dutta, Ning Sun, Sivakumar Pattathil, N. V. S. N. Murthy Konda, Angelo Gabriel Peralta, Blake A. Simmons and Seema Singh
    Citation: Biotechnology for Biofuels 2016 9:160
  5. Phospholipase A1 is an enzyme that hydrolyzes phospholipids at the sn-1 position. It has potential applications across diverse fields including food, pharmaceutical, and biofuel industries. Although there has bee...

    Authors: Hye Jin Lim, Yu Jin Park, Yeon Jae Jang, Ji Eun Choi, Joon Young Oh, Ji Hyun Park, Jae Kwang Song and Dong-Myung Kim
    Citation: Biotechnology for Biofuels 2016 9:159
  6. Engineering of the yeast Saccharomyces cerevisiae for improved utilization of pentose sugars is vital for cost-efficient cellulosic bioethanol production. Although endogenous hexose transporters (Hxt) can be engi...

    Authors: Jeroen G. Nijland, Erwin Vos, Hyun Yong Shin, Paul P. de Waal, Paul Klaassen and Arnold J. M. Driessen
    Citation: Biotechnology for Biofuels 2016 9:158
  7. Lignocellulosic ethanol production involves major steps such as thermochemical pretreatment of biomass, enzymatic hydrolysis of pre-treated biomass and the fermentation of released sugars into ethanol. At leas...

    Authors: Anu Jose Mattam, Arindam Kuila, Niranjan Suralikerimath, Nettem Choudary, Peddy V. C. Rao and Harshad Ravindra Velankar
    Citation: Biotechnology for Biofuels 2016 9:157
  8. Biofuel production from conversion of biomass is indispensable in the portfolio of renewable energies. Complex microbial communities are involved in the anaerobic digestion process of plant material, agricultu...

    Authors: Yvonne Stolze, Andreas Bremges, Madis Rumming, Christian Henke, Irena Maus, Alfred PĂĽhler, Alexander Sczyrba and Andreas SchlĂĽter
    Citation: Biotechnology for Biofuels 2016 9:156
  9. Methane yield and biogas productivity of biogas plants (BGPs) depend on microbial community structure and function, substrate supply, and general biogas process parameters. So far, however, relatively little i...

    Authors: R. Heyer, D. Benndorf, F. Kohrs, J. De Vrieze, N. Boon, M. Hoffmann, E. Rapp, Andreas SchlĂĽter, Alexander Sczyrba and U. Reichl
    Citation: Biotechnology for Biofuels 2016 9:155
  10. Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of t...

    Authors: Daniela Castiglia, Lorenza Sannino, Loredana Marcolongo, Elena Ionata, Rachele Tamburino, Angelo De Stradis, Beatrice Cobucci-Ponzano, Marco Moracci, Francesco La Cara and Nunzia Scotti
    Citation: Biotechnology for Biofuels 2016 9:154
  11. Second-generation ethanol (2G-bioethanol) uses lignocellulosic feedstocks for ethanol production. Sugarcane is one among the most suitable crops for biofuel production. Its juice is extracted for sugar product...

    Authors: Maria Thereza Bazzo Martins, Wagner Rodrigo de Souza, Bárbara Andrade Dias Brito da Cunha, Marcos Fernando Basso, Nelson Geraldo de Oliveira, Felipe Vinecky, Polyana Kelly Martins, Patrícia Abrão de Oliveira, Bruna Cersózimo Arenque-Musa, Amanda Pereira de Souza, Marcos Silveira Buckeridge, Adilson Kenji Kobayashi, Betania Ferraz Quirino and Hugo Bruno Correa Molinari
    Citation: Biotechnology for Biofuels 2016 9:153
  12. Steam explosion pretreatment has been examined in many studies for enhancing the enzymatic digestibility of lignocellulosic biomass and is currently the most common pretreatment method in commercial biorefiner...

    Authors: Thomas Pielhop, Janick Amgarten, Philipp Rudolf von Rohr and Michael H. Studer
    Citation: Biotechnology for Biofuels 2016 9:152
  13. Microalgae are emerging as suitable feedstock for renewable biofuel production and providing a promising way to alleviate green house gas CO2. Characterizing the metabolic pathways involved in the biosynthesis of...

    Authors: Huifeng Peng, Dong Wei, Gu Chen and Feng Chen
    Citation: Biotechnology for Biofuels 2016 9:151
  14. Intermediates from processing sugar beets are considered an attractive feedstock for ethanol fermentation due to their high fermentable sugar content. In particular, medium prepared from raw sugar beet juice s...

    Authors: Piotr Dziugan, Maria Balcerek, Michal J. Binczarski, Dorota Kregiel, Marcin Kucner, Alina Kunicka-Styczynska, Katarzyna Pielech-Przybylska, Krzysztof Smigielski and Izabela A. Witonska
    Citation: Biotechnology for Biofuels 2016 9:150
  15. The oleaginous yeast, Yarrowia lipolytica, has been utilized as an industrial host for about 60 years for various applications. Recently, the metabolic engineering of this host has become increasingly popular due...

    Authors: Gabriel M. Rodriguez, Murtaza Shabbir Hussain, Lauren Gambill, Difeng Gao, Allison Yaguchi and Mark Blenner
    Citation: Biotechnology for Biofuels 2016 9:149
  16. Global climate change and fossil fuels limitations have boosted the demand for robust and efficient microbial factories for the manufacturing of bio-based products from renewable feedstocks. In this regard, ef...

    Authors: Jasper Sloothaak, Juan Antonio Tamayo-Ramos, Dorett I. Odoni, Thanaporn Laothanachareon, Christian Derntl, Astrid R. Mach-Aigner, Vitor A. P. Martins dos Santos and Peter J. Schaap
    Citation: Biotechnology for Biofuels 2016 9:148
  17. β-Glucosidase is an important member of the biomass-degrading enzyme system, and plays vital roles in enzymatic saccharification for biofuels production. Candidates with high activity and great stability over ...

    Authors: Wei Xia, Xinxin Xu, Lichun Qian, Pengjun Shi, Yingguo Bai, Huiying Luo, Rui Ma and Bin Yao
    Citation: Biotechnology for Biofuels 2016 9:147
  18. Butanol is not only an important solvent and chemical intermediate in food and pharmaceutical industries, but also considered as an advanced biofuel. Recently, there have been resurging interests in producing ...

    Authors: Chuang Xue, Zixuan Wang, Shudong Wang, Xiaotong Zhang, Lijie Chen, Ying Mu and Fengwu Bai
    Citation: Biotechnology for Biofuels 2016 9:146
  19. Sugarcane is one of the world’s most profitable crops. Waste steam-exploded sugarcane bagasse (SEB) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels. In nature, fu...

    Authors: Neil Andrew Brown, Laure N. A. Ries, Thaila F. Reis, Ranjith Rajendran, Renato Augusto Corrêa dos Santos, Gordon Ramage, Diego Mauricio Riaño-Pachón and Gustavo H. Goldman
    Citation: Biotechnology for Biofuels 2016 9:145
  20. During strategic planning of a biogas plant, the local availability of resources for start-up and operation should be taken into consideration for a cost-efficient process. Because most bioethanol/sugar indust...

    Authors: Athaydes Francisco Leite, Leandro Janke, Hauke Harms, Hans-Hermann Richnow and Marcell Nikolausz
    Citation: Biotechnology for Biofuels 2016 9:144
  21. Sugarcane, a tropical C4 perennial crop, is capable of producing 30–100 tons or more of biomass per hectare annually. The lignocellulosic residue remaining after sugar extraction is currently underutilized and...

    Authors: Charleson R. Poovaiah, William P. Bewg, Wu Lan, John Ralph and Heather D. Coleman
    Citation: Biotechnology for Biofuels 2016 9:143
  22. The requirement for efficient and green conversion technologies has prompted hydrothermal processing as a promising treatment option for sustainable biorefinery industry. The treatment has been applied to proc...

    Authors: Zhi-Wen Wang, Ming-Qiang Zhu, Ming-Fei Li, Jun-Qi Wang, Qin Wei and Run-Cang Sun
    Citation: Biotechnology for Biofuels 2016 9:142
  23. Conceptually, multi-functional enzymes are attractive because in the case of complex polymer hydrolysis having two or more activities defined by a single enzyme offers the possibility of synergy and reduced en...

    Authors: Hanen Bouraoui, Marie-Laure Desrousseaux, Eleni Ioannou, Pablo Alvira, Mohamed Manaï, Caroline Rémond, Claire Dumon, Narcis Fernandez-Fuentes and Michael J. O’Donohue
    Citation: Biotechnology for Biofuels 2016 9:140
  24. Caffeoyl shikimate esterase (CSE) was recently characterized as an enzyme central to the lignin biosynthetic pathway in Arabidopsis thaliana. The cse-2 loss-of-function mutant shows a typical phenotype of lignin-...

    Authors: LĂ­via Vargas, Igor Cesarino, Ruben Vanholme, Wannes Voorend, Marina de Lyra Soriano Saleme, Kris Morreel and Wout Boerjan
    Citation: Biotechnology for Biofuels 2016 9:139
  25. Energy shortage has become a global problem. Production of biofuels from renewable biomass resources is an inevitable trend of sustainable development. Cellulose is the most abundant and renewable resource in ...

    Authors: Chao Yang, Yu Xia, Hong Qu, An-Dong Li, Ruihua Liu, Yubo Wang and Tong Zhang
    Citation: Biotechnology for Biofuels 2016 9:138
  26. Consolidated bioprocessing (CBP), integrating cellulase production, cellulose saccharification, and fermentation into one step has been widely considered as the ultimate low-cost configuration for producing se...

    Authors: Li-Hai Fan, Zi-Jian Zhang, Sen Mei, Yang-Yang Lu, Mei Li, Zai-Yu Wang, Jian-Guo Yang, Shang-Tian Yang and Tian-Wei Tan
    Citation: Biotechnology for Biofuels 2016 9:137
  27. Camelina sativa is an emerging dedicated oilseed crop designed for biofuel and biodiesel applications as well as a source for edible and general-purpose oils. Such valuable oilseed cro...

    Authors: Hesham M. Abdullah, Parisa Akbari, Bibin Paulose, Danny Schnell, Weipeng Qi, Yeonhwa Park, Ashwani Pareek and Om Parkash Dhankher
    Citation: Biotechnology for Biofuels 2016 9:136
  28. In order to establish a cost-efficient biodiesel biorefinery, valorization of its main by-product, crude glycerol, is imperative. Recently, Ustilago trichophora TZ1 was found to efficiently produce malic acid fro...

    Authors: Thiemo Zambanini, Wiebke Kleineberg, Eda Sarikaya, Joerg M. Buescher, Guido Meurer, Nick Wierckx and Lars M. Blank
    Citation: Biotechnology for Biofuels 2016 9:135
  29. Poly-Îł-glutamic acid (Îł-PGA) is a naturally occurring biopolymer made from repeating units of l-glutamic acid, d-glutamic acid, or both. Since some bacteria are capable of vigorous Îł-PGA biosynthesis from renewab...

    Authors: Zhiting Luo, Yuan Guo, Jidong Liu, Hua Qiu, Mouming Zhao, Wei Zou and Shubo Li
    Citation: Biotechnology for Biofuels 2016 9:134
  30. Glycoside hydrolases (GH) targeting cellulose, xylan, and chitin are common in the bacterial genomes that have been sequenced. Little is known, however, about the architecture of multi-domain and multi-activit...

    Authors: Darrian Talamantes, Nazmehr Biabini, Hoang Dang, Kenza Abdoun and Renaud Berlemont
    Citation: Biotechnology for Biofuels 2016 9:133
  31. The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is a widely used industrial host organism for protein production. In industrial cultivations, it can produce over 100 g/l of extracellular ...

    Authors: Tiina M. Pakula, Heli Nygren, Dorothee Barth, Markus Heinonen, Sandra Castillo, Merja Penttilä and Mikko Arvas
    Citation: Biotechnology for Biofuels 2016 9:132
  32. Infrastructure compatible hydrocarbon biofuel proposed to qualify as renewable transportation fuel under the U.S. Energy Independence and Security Act of 2007 and Renewable Fuel Standard (RFS2) is evaluated. T...

    Authors: Jordan T. Crawford, Chin Wei Shan, Erik Budsberg, Hannah Morgan, Renata Bura and Rick Gustafson
    Citation: Biotechnology for Biofuels 2016 9:141
  33. Industrial biotechnology that is able to provide environmentally friendly bio-based products has attracted more attention in replacing petroleum-based industries. Currently, most of the carbon sources used for...

    Authors: Qiang Fei, Marykate O’Brien, Robert Nelson, Xiaowen Chen, Andrew Lowell and Nancy Dowe
    Citation: Biotechnology for Biofuels 2016 9:130
  34. C5–C8 medium-chain carboxylic acids are valuable chemicals as the precursors of various chemicals and transport fuels. However, only a few strict anaerobes have been discovered to produce them and their produc...

    Authors: Byoung Seung Jeon, Okkyoung Choi, Youngsoon Um and Byoung-In Sang
    Citation: Biotechnology for Biofuels 2016 9:129
  35. Materials rich in lignocellulose, such as straw, are abundant, cheap and highly interesting for biogas production. However, the complex structure of lignocellulose is difficult for microbial cellulolytic enzym...

    Authors: Li Sun, Tong Liu, Bettina MĂĽller and Anna SchnĂĽrer
    Citation: Biotechnology for Biofuels 2016 9:128
  36. SWEET is a newly identified family of sugar transporters. Although SWEET transporters have been characterized by using Arabidopsis and rice, very little knowledge of sucrose accumulation in the stem region is ...

    Authors: Hiroshi Mizuno, Shigemitsu Kasuga and Hiroyuki Kawahigashi
    Citation: Biotechnology for Biofuels 2016 9:127
  37. Coordination of synthesis and assembly of the polymeric components of cell walls is essential for plant growth and development. Given the degree of co-mingling and cross-linking among cell wall components, cel...

    Authors: Jiliang Liu, Jeong Im Kim, Joanne C. Cusumano, Clint Chapple, Nagarajan Venugopalan, Robert F. Fischetti and Lee Makowski
    Citation: Biotechnology for Biofuels 2016 9:126
  38. The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equiva...

    Authors: Christopher D. Herring, William R. Kenealy, A. Joe Shaw, Sean F. Covalla, Daniel G. Olson, Jiayi Zhang, W. Ryan Sillers, Vasiliki Tsakraklides, John S. Bardsley, Stephen R. Rogers, Philip G. Thorne, Jessica P. Johnson, Abigail Foster, Indraneel D. Shikhare, Dawn M. Klingeman, Steven D. Brown…
    Citation: Biotechnology for Biofuels 2016 9:125
  39. β-Glucanase is one of the most extensively used biocatalysts in biofuel, food and animal feed industries. However, the poor thermostability and low catalytic efficiency of most reported β-glucanases limit thei...

    Authors: Shuai You, Tao Tu, Lujia Zhang, Yuan Wang, Huoqing Huang, Rui Ma, Pengjun Shi, Yingguo Bai, Xiaoyun Su, Zhemin Lin, Huiying Luo and Bin Yao
    Citation: Biotechnology for Biofuels 2016 9:124
  40. Commercial production of microalgal biodiesel is not yet economically viable, largely because of low storage lipid yield in microalgae mass cultivation. Selection of lipid-rich microalgae, thus, becomes one of...

    Authors: Xiaobin Wen, Kui Du, Zhongjie Wang, Xinan Peng, Liming Luo, Huanping Tao, Yan Xu, Dan Zhang, Yahong Geng and Yeguang Li
    Citation: Biotechnology for Biofuels 2016 9:123
  41. Biorefineries serve to efficiently utilize biomass and their by-products. Algal biorefineries are designed to generate bioproducts for commercial use. Due to the high carbohydrate content of algal biomass, bio...

    Authors: Fernando Masarin, Fernando Roberto Paz Cedeno, Eddyn Gabriel Solorzano Chavez, Levi Ezequiel de Oliveira, Valéria Cress Gelli and Rubens Monti
    Citation: Biotechnology for Biofuels 2016 9:122
  42. The diverse microbial communities in agricultural biogas fermenters are assumed to be well adapted for the anaerobic transformation of plant biomass to methane. Compared to natural systems, biogas reactors are...

    Authors: Simon GĂĽllert, Martin A. Fischer, Dmitrij Turaev, Britta Noebauer, Nele Ilmberger, Bernd Wemheuer, Malik Alawi, Thomas Rattei, Rolf Daniel, Ruth A. Schmitz, Adam Grundhoff and Wolfgang R. Streit
    Citation: Biotechnology for Biofuels 2016 9:121
  43. This study aims to chemically characterize thin stillage derived from lignocellulosic biomass distillation residues in terms of organic strength, nutrient, and mineral content. The feasibility of performing an...

    Authors: Margreet J. Oosterkamp, Celia Méndez-García, Chang-H. Kim, Stefan Bauer, Ana B. Ibáñez, Sabrina Zimmerman, Pei-Ying Hong, Isaac K. Cann and Roderick I. Mackie
    Citation: Biotechnology for Biofuels 2016 9:120
  44. Saccharification of lignocellulosic material by xylanases and other glycoside hydrolases is generally conducted at high concentrations of the final reaction products, which frequently inhibit the enzymes used ...

    Authors: Lucas Ferreira Ribeiro, Jennifer Tullman, Nathan Nicholes, SĂ©rgio Ruschi Bergamachi Silva, Davi Serradella Vieira, Marc Ostermeier and Richard John Ward
    Citation: Biotechnology for Biofuels 2016 9:119
  45. In the bioconversion of lignocellulosic substrates, the adsorption behavior of cellulase onto lignin has a negative effect on enzymatic hydrolysis of cellulose, decreasing glucose production during enzymatic h...

    Authors: Xianqin Lu, Xiaoju Zheng, Xuezhi Li and Jian Zhao
    Citation: Biotechnology for Biofuels 2016 9:118
  46. 2,3-Butanediol (2,3-BD) can be used as a liquid fuel additive to replace petroleum oil, and as an important platform chemical in the pharmaceutical and plastic industries. Microbial production of 2,3-BD by Bacill...

    Authors: Yimin Qiu, Jinyan Zhang, Lu Li, Zhiyou Wen, Christopher T. Nomura, Shuilin Wu and Shouwen Chen
    Citation: Biotechnology for Biofuels 2016 9:117
  47. Biofuel production from plant cell walls offers the potential for sustainable and economically attractive alternatives to petroleum-based products. Fuels from cellulosic biomass are particularly promising, but...

    Authors: Liang Tian, Beth Papanek, Daniel G. Olson, Thomas Rydzak, Evert K. Holwerda, Tianyong Zheng, Jilai Zhou, Marybeth Maloney, Nannan Jiang, Richard J. Giannone, Robert L. Hettich, Adam M. Guss and Lee R. Lynd
    Citation: Biotechnology for Biofuels 2016 9:116
  48. Escherichia coli has been explored as a platform host strain for biofuels production such as butanol. However, the severe toxicity of butanol is considered to be one major limitation f...

    Authors: Hai-Ming Si, Fa Zhang, An-Ning Wu, Rui-Zhi Han, Guo-Chao Xu and Ye Ni
    Citation: Biotechnology for Biofuels 2016 9:114