Page 1 of 11
Filamentous fungus Trichoderma reesei...has been widely used as a workhorse for cellulase and xylanase productions. Xylanase has been reported as the crucial accessory ... an increasing demand for the high yield ...
The enzymatic hydrolysis step converting lignocellulosic materials into fermentable sugars is recognized as one of the major limiting steps in biomass-to-ethanol process due to the low efficiency of enzymes and t...
We and other workers have shown that accessory enzymes, such as β-glucosidase, xylanase, and cellulase cofactors, such as GH61,...
The filamentous fungus Penicillium oxalicum is a potential alternative to Trichoderma reesei for industrial production of a complete cellulolytic enzyme system for a bio-refinery. Comparative omics approaches can...
A semi-rational protein fusion strategy was used to create a protein fusion library where the Bacillus subtilis GH11 xylanase A (XynA) was inserted at 144...Escherichia coli xylose binding protein (XBP). Two XynA...
In this study, cellulase was supplemented with xylanase and β-xylosidase to boost conversion of ... the less inhibitory xylose. Although addition of xylanase and β-xylosidase did not necessarily enhance ... of a ...
Here, we report on a T. reesei...strain, which exhibits a very high level of xylanase expression regardless if inducing substances (e.g ... single point mutation in the gene encoding the Xylanase regulator 1 (Xyr...
Xylanases have drawn much attention owing to possessing ... various industrial applications. However, the applicability of xylanases, exemplified by the production of bioethanol and ... a mesophilic glycoside hyd...
This study presents the investigation of the thermostable, dual-function xylanase-glucuronoyl esterase enzyme CkXyn10C-GE15A from the hyperthermophilic bacterium Caldicellulosiruptor kristjanssonii. Biochemical c...
The search for novel thermostable xylanases for industrial use has intensified in recent ... expression and biochemical characterization of a novel thermostable xylanase (GH10) from the thermophilic fungus Malbra...
Improving the hydrolytic performance of hemicellulases on lignocellulosic biomass is of considerable importance for second-generation biorefining. To address this problem, and also to gain greater understanding o...
Xylanase is an important component of hemicellulase enzyme ... hemicellulose into xylooligosaccharides (XOs), high thermostable xylanase has been the focus of much recent...
Two carbohydrate esterases with similar architectures, each comprising two distinct physically linked catalytic domains from families 1 (CE1) and 6 (CE6), were selected from xylan-targeting polysaccharide util...
The well-known industrial fungus Trichoderma reesei...has an excellent capability of secreting a large amount of cellulases and xylanases. The induced expression of cellulase and xylanase genes is tightly control...
The psychrophilic lignocellulosic enzymes system of Cladosporium cladosporioides Ch2-2 including ligninase and polysaccharide hydrolases was suitable for selective delignification and efficient saccharification o...
The filamentous fungus Trichoderma reesei...is widely utilized in industry for cellulase production, but its xylanase activity must be improved to enhance the ... both cellulase and hemi-cellulase genes. Specific...
Trichoderma reesei...is known for its ability to produce large amounts of extracellular proteins and is one of the most important industrially used filamentous fungus. Xylanase regulator 1 (XYR1) is the master...
We identified a copper transporter encoding gene tcu1 (jgi:Trire2:52315) in T. reesei, the transcription of which was highly responsive to copper availability. Whereas excess copper repressed the expression of tc...
The transition to a more environmentally friendly economy has prompted studies of modern biorefineries, including the utilization of low-value lignocellulose. The major challenge facing the widespread applicat...
Replacing fossil fuel with renewable sources such as lignocellulosic biomass is currently a promising alternative for obtaining biofuel and for fighting against the consequences of climate change. However, the re...
Xylanase is one of the most extensively used ... its applications. Therefore, improving the properties of xylanases to enable synergistic degradation of lignocellulosic biomass...
Enzymatic removal of hemicellulose components such as xylan is an important factor for maintaining high glucose conversion from lignocelluloses subjected to low-severity pretreatment. Supplementation of xylanase ...
Saccharification of lignocellulosic material by xylanases and other glycoside hydrolases is generally conducted ... recombination strategy, we have fused the GH11 xylanase from Bacillus subtilis (XynA) with the x...
Xylanases have been widely employed in many industrial processes, and thermophilic xylanases are in great demand for meeting the ... , a glycoside hydrolase (GH) family 11 xylanase from the ruminal fungus Neocall...
A novel GH10 enzyme (XynA) identified from a xylanolytic strain Bacillus sp. KW1 was cloned and expressed. Its optimal pH and temperature were determined to be pH 6.0 and 65 °C. Stability analyses revealed that X...
Improving the hydrolytic performance of hemicellulases to degrade lignocellulosic biomass is of considerable importance for second-generation biorefinery. Xylanase, as the crucial hemicellulase, must be ... in th...
In the present study, the only annotated extracellular endo-β-1,4-xylanase (Xyn10B) with multidomain structures in C. lactoaceticus genome was biochemically characterized. Xyn10B contains three N-terminal consecu...
Improving the hydrolytic performance of xylanolytic enzymes on arabinoxylan is of importance in the ethanol fermentation industry. Supplementation of debranching (arabinofuranosidase) and depolymerizing (xylanase
Xylan removal by bacterial pretreatments has been confirmed to increase the digestibility of biomass. Here, an effective xylan removal technique has been developed to enhance the digestibility of wheat straw and ...
Solid-state fermentation (SSF) mimics the natural decay environment of soil fungi and can be employed to investigate the production of plant biomass-degrading enzymes. However, knowledge on the transcriptional...
In this study, a microbial community from compost was grown in minimal medium with sugarcane bagasse sugarcane bagasse as the sole carbon source. Solid-state nuclear magnetic resonance was used to monitor lignoce...
Penicillium...sp. strain KSM-F532 produced the most effective enzyme to promote the saccharification of alkaline-pretreated bagasse. Biomass-degrading enzymes from strain KSM-F532 were...
Through genetic and genomic analyses, we show that the 16 ascospores are generated via meiosis followed by two rounds of postmeiotic mitosis. We also found that the haploid genomes of CBS999.97(1-2) and QM6a a...
The main representatives of hemicellulose are xylans, usually decorated β-1,4-linked d-xylose polymers, which are hydrolyzed by xylanases. The efficient utilization and complete hydrolysis of ... . The glycoside ...
Xylo-oligosaccharides (XOS) are considered as a promising type of prebiotics that can be used in foods, feeds, and healthcare products. Xylanases play a key role in the production...Methanobrevibacter and increas...
The changes in lignocellulolytic enzyme activity, gene expression involving (hemi)cellulolytic enzymes, and the secretome of cultures grown on Avicel (A), xylan (X), or a mixture of both (AX) were studied. The...
Xylanase, an important accessory enzyme that acts in...
The optimal AFEX conditions for all stover fractions, regardless of harvest period, were: 1.5 (g NH3 g-1...biomass); 60% moisture content (dry-weight basis; dwb), 90°C and 5 min residence time. Enzymatic hydrolys...
In the current study, the high spatial resolution (about 30 nm) of scanning transmission X-ray microscopy (STXM), and the detection sensitivity (ppm) of time-of-flight secondary ion mass spectrometry (ToF-SIMS), ...
Enzymatic hydrolysis of lignocellulosic biomass into platform sugars can be enhanced by the addition of accessory enzymes, such as xylanases. Lignin from steam pretreated biomasses is known ... by four glycoside ...
In the hydrolysis of lignocellulosic materials, thermostable enzymes decrease the amount of enzyme needed due to higher specific activity and elongate the hydrolysis time due to improved stability. For cost-effic...
Bacillus subtilis (B. subtilis) RLI2019 was isolated from the intestine of Reticulitermes labralis.... The strain showed comprehensive enzyme activities related to lignocellulose degradation, which were estimated...
We have used transcriptional profiling data from T. reesei cultures in which cellulase/hemicellulase production was induced by the addition of different lignocellulose-derived materials to identify putative novel...
The selected enzymes, endoglucanase, endo-β-1,4-xylanase and β-glucosidase, were expressed in tobacco ... protein to thylakoids. Endo-β-1,4-xylanase and β-glucosidase, produced at very high ... expressed enzymes ...
An integrated process which combines dissolving pulp production with the recovery of excellent sustainable biofuel and biochemical feedstocks is presented in this work. Pretreatment at 95 °C with 12% NaOH charge ...
The optimal doses of cellulase and xylanase enzymes were 23 FPU and 62 IU/...Saccharomyces cerevisiae resulted in 0.172 g ethanol/g biomass after 24 h, equivalent to 80.9% of the maximum theoretical yield (based ...
Although high concentrations of Zn2+ severely suppressed the extension of T. reesei mycelia, the application of 1–4 mM Zn2+ enhanced cellulase and xylanase production in the high-yielding cellulase-producing...T....
Lactobacillus plantarum is an attractive candidate for metabolic engineering towards bioprocessing of lignocellulosic biomass to ethanol or polylactic acid, as its natural characteristics include high ethanol and...
Ethanol addition improved p-coumaric acid release and delignification efficiency, but significantly reduced hemicellulose deconstruction in NaOH–ethanol pretreatment. Optimization using the response surface metho...
Cellulolytic A. fumigatus...Z5 was grown in the presence of glucose (Gl), Avicel (Av) and rice straw (RS), and the activities of several lignocellulosic enzymes were determined with chromatometry method. The maxi...