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  1. Efficient use of glucose and xylose is a key for the economic production of lignocellulosic biofuels and biochemicals, and different recombinant strains have been constructed for xylose utilization including t...

    Authors: Jiyun Lou, Jingwen Wang, Yongfu Yang, Qing Yang, Runxia LI, Mimi Hu, Qiaoning He, Jun Du, Xia Wang, Mian Li and Shihui Yang
    Citation: Biotechnology for Biofuels 2021 14:231
  2. Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain d...

    Authors: Na Wu, Jiahui Zhang, Wen Ou, Yaru Chen, Ru Wang, Ke Li, Xiao-man Sun, Yingfeng Li, Qing Xu and He Huang
    Citation: Biotechnology for Biofuels 2021 14:230
  3. The ability of oxidative enzyme-producing micro-organisms to efficiently valorize organic pollutants is critical in this context. Yeasts are promising enzyme producers with potential applications in waste mana...

    Authors: Rania Al-Tohamy, Jianzhong Sun, Maha A. Khalil, Michael Kornaros and Sameh Samir Ali
    Citation: Biotechnology for Biofuels 2021 14:229
  4. Lignocellulosic biomass is an attractive and sustainable alternative to petroleum-based feedstock for the production of a range of biochemicals, and pretreatment is generally regarded as indispensable for its ...

    Authors: Lihua Zou, Shuiping Ouyang, Yueli Hu, Zhaojuan Zheng and Jia Ouyang
    Citation: Biotechnology for Biofuels 2021 14:227
  5. Chinese hickory shell, a by-product of the food industry, is still not utilized and urgent to develop sustainable technologies for its valorization. This research focuses on the systematical evaluation of degr...

    Authors: Zhi-Kun Wang, Caoxing Huang, Jun-Lei Zhong, Yi Wang, Lv Tang, Bing Li, Jian-Jun Sheng, Liang Chen, Shaolong Sun and XiaoJun Shen
    Citation: Biotechnology for Biofuels 2021 14:226
  6. Starch is one of the most important renewable polysaccharides in nature for production of bio-ethanol. The starch saccharification step facilitates the depolymerization of starch to yield glucose for biofuels ...

    Authors: Wenzhu Guo, Jianhua Yang, Tianchen Huang, Dandan Liu, Qian Liu, Jingen Li, Wenliang Sun, Xingji Wang, Leilei Zhu and Chaoguang Tian
    Citation: Biotechnology for Biofuels 2021 14:225
  7. Peroxyacetic acid involved chemical pretreatment is effective in lignocellulose deconstruction and oxidation. However, these peroxyacetic acid are usually artificially added. Our previous work has shown that t...

    Authors: Dong Tian, Yiyi Chen, Fei Shen, Maoyuan Luo, Mei Huang, Jinguang Hu, Yanzong Zhang, Shihuai Deng and Li Zhao
    Citation: Biotechnology for Biofuels 2021 14:224
  8. Xylose contained in lignocellulosic biomass is an attractive carbon substrate for economically viable conversion to bioethanol. Extensive research has been conducted on xylose fermentation using recombinant Sacch...

    Authors: Jung-Hoon Bae, Mi-Jin Kim, Bong Hyun Sung, Yong-Su Jin and Jung-Hoon Sohn
    Citation: Biotechnology for Biofuels 2021 14:223
  9. High-temperature bioethanol production benefits from yeast thermotolerance. Salt stress could induce obvious cross-protection against heat stress of Pichia kudriavzevii, contributing to the improvement of its the...

    Authors: Chunsheng Li, Qiuying Liu, Yueqi Wang, Xianqing Yang, Shengjun Chen, Yongqiang Zhao, Yanyan Wu and Laihao Li
    Citation: Biotechnology for Biofuels 2021 14:222
  10. As one of the clean and sustainable energies, lignocellulosic ethanol has achieved much attention around the world. The production of lignocellulosic ethanol does not compete with people for food, while the co...

    Authors: Lingling Sun, Bo Wu, Zengqin Zhang, Jing Yan, Panting Liu, Chao Song, Samina Shabbir, Qili Zhu, Shihui Yang, Nan Peng, Mingxiong He and Furong Tan
    Citation: Biotechnology for Biofuels 2021 14:221
  11. Hemicellulose acts as one factor contributing to the recalcitrance of lignocellulose that prevents cellulases to degrade the cellulose efficiently even in low quantities. Supplement of hemicellulases can enhan...

    Authors: Qing Lan, Yitong Duan, Pingping Wu, Xueyin Li, Yao Yu, Bo Shi, Jungang Zhou and Hong Lu
    Citation: Biotechnology for Biofuels 2021 14:220
  12. Cellulase plays a key role in converting cellulosic biomass into fermentable sugar to produce chemicals and fuels, which is generally produced by filamentous fungi. However, most of the filamentous fungi obtai...

    Authors: Zhi-Qing Peng, Chuang Li, Yi Lin, Sheng-Shan Wu, Li-Hui Gan, Jian Liu, Shu-Liang Yang, Xian-Hai Zeng and Lu Lin
    Citation: Biotechnology for Biofuels 2021 14:219
  13. Medium-chain-length α,ω-diols (mcl-diols) are important building blocks in polymer production. Recently, microbial mcl-diol production from alkanes was achieved in E. coli (albeit at low rates) using the alkane m...

    Authors: Chunzhe Lu, Christos Batianis, Edward Ofori Akwafo, Rene H. Wijffels, Vitor A. P. Martins dos Santos and Ruud A. Weusthuis
    Citation: Biotechnology for Biofuels 2021 14:218
  14. The development of bioenergy crops with reduced recalcitrance to enzymatic degradation represents an important challenge to enable the sustainable production of advanced biofuels and bioproducts. Biomass recal...

    Authors: Yang Tian, Chien-Yuan Lin, Joon-Hyun Park, Chuan-Yin Wu, Ramu Kakumanu, Venkataramana R. Pidatala, Khanh M. Vuu, Alberto Rodriguez, Patrick M. Shih, Edward E. K. Baidoo, Stephen Temple, Blake A. Simmons, John M. Gladden, Henrik V. Scheller and Aymerick Eudes
    Citation: Biotechnology for Biofuels 2021 14:217
  15. 1,2-Propanediol is an important building block as a component used in the manufacture of unsaturated polyester resin, antifreeze, biofuel, nonionic detergent, etc. Commercial production of 1,2-propanediol thro...

    Authors: Yuan-ming Tao, Chong-yang Bu, Li-hua Zou, Yue-li Hu, Zhao-Juan Zheng and Jia Ouyang
    Citation: Biotechnology for Biofuels 2021 14:216
  16. Rapeseed is the third-largest oilseed crop after soybeans and palm that produces vegetable oil for human consumption and biofuel for industrial production. Silique length (SL) is an important trait that is str...

    Authors: Jia Wang, Yueling Fan, Lin Mao, Cunmin Qu, Kun Lu, Jiana Li and Liezhao Liu
    Citation: Biotechnology for Biofuels 2021 14:214
  17. With the continuous depletion of energy sources globally and serious concern regarding environmental degradation by the use of fossil fuel, biodiesel may play a key transponder. Biodiesel blended with diesel f...

    Authors: Rajendra Kukana and O. P. Jakhar
    Citation: Biotechnology for Biofuels 2021 14:213
  18. CO2 valorization is one of the effective methods to solve current environmental and energy problems, in which microbial electrosynthesis (MES) system has proved feasible and efficient. Cupriviadus necator (Ralsto...

    Authors: Haojie Pan, Jia Wang, Haoliang Wu, Zhongjian Li and Jiazhang Lian
    Citation: Biotechnology for Biofuels 2021 14:212
  19. The brewer’s yeast Saccharomyces cerevisiae is exploited in several industrial processes, ranging from food and beverage fermentation to the production of biofuels, pharmaceuticals and complex chemicals. The larg...

    Authors: Ping-Wei Ho, Supinya Piampongsant, Brigida Gallone, Andrea Del Cortona, Pieter-Jan Peeters, Frank Reijbroek, Jules Verbaet, Beatriz Herrera, Jeroen Cortebeeck, Robbe Nolmans, Veerle Saels, Jan Steensels, Daniel F. Jarosz and Kevin J. Verstrepen
    Citation: Biotechnology for Biofuels 2021 14:211
  20. The members of the genus Caldicellulosiruptor have the potential for future integration into a biorefinery system due to their capacity to generate hydrogen close to the theoretical limit of 4 mol H2/mol hexose, ...

    Authors: Eoin Byrne, Johanna Björkmalm, James P. Bostick, Krishnan Sreenivas, Karin Willquist and Ed W. J. van Niel
    Citation: Biotechnology for Biofuels 2021 14:210
  21. Despite enormous challenges in accessing sustainable energy supplies and advanced energy technologies, Ethiopia has one of the world's fastest growing economies. The development of renewable energy technology ...

    Authors: Natei Ermias Benti, Gamachis Sakata Gurmesa, Tegenu Argaw, Abreham Berta Aneseyee, Solomon Gunta, Gashaw Beyene Kassahun, Genene Shiferaw Aga and Ashenafi Abebe Asfaw
    Citation: Biotechnology for Biofuels 2021 14:209
  22. Zymomonas mobilis is a natural ethanologen with many desirable characteristics, making it an ideal platform for future biorefineries. Recently, an endogenous CRISPR-based genome editing tool has been developed fo...

    Authors: Xin Sui, Xiaojie Wang, Tao Liu, Qing Ye, Bo Wu, Guoquan Hu, Shihui Yang, Mingxiong He and Nan Peng
    Citation: Biotechnology for Biofuels 2021 14:208
  23. Direct reductive amination of prochiral 2-oxo-4-phenylbutyric acid (2-OPBA) catalyzed by phenylalanine dehydrogenase (PheDH) is highly attractive in the synthesis of the pharmaceutical chiral building block l-hom...

    Authors: Tao Wu, Xiaoqing Mu, Yuyan Xue, Yan Xu and Yao Nie
    Citation: Biotechnology for Biofuels 2021 14:207
  24. Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are scarc...

    Authors: Chao Fan, Wei Zhang, Xiaoyun Su, Wangli Ji, Huiying Luo, Yuhong Zhang, Bo Liu, Bin Yao, Huoqing Huang and Xinxin Xu
    Citation: Biotechnology for Biofuels 2021 14:206
  25. Enzymatic hydrolysis of lignocellulose for bioethanol production shows a great potential to remit the rapid consumption of fossil fuels, given the fact that lignocellulose feedstocks are abundant, cost-efficie...

    Authors: Yufeng Yuan, Bo Jiang, Hui Chen, Wenjuan Wu, Shufang Wu, Yongcan Jin and Huining Xiao
    Citation: Biotechnology for Biofuels 2021 14:205
  26. Clostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock, such as syngas. Achieving improved ethanol and butanol production in the strain for industrial a...

    Authors: Xiangfei Li, Rumeng Han, Teng Bao, Tolbert Osire, Xian Zhang, Meijuan Xu, Taowei Yang and Zhiming Rao
    Citation: Biotechnology for Biofuels 2021 14:204
  27. Butyl acetate has shown wide applications in food, cosmetics, medicine, and biofuel sectors. These short-chain fatty acid esters can be produced by either chemical or biological synthetic process with correspo...

    Authors: Yang Lv, Yujia Jiang, Jiasheng Lu, Hao Gao, Weiliang Dong, Jie Zhou, Wenming Zhang, Fengxue Xin and Min Jiang
    Citation: Biotechnology for Biofuels 2021 14:203
  28. Glucoamylase is an important industrial enzyme in the saccharification of starch into glucose. However, its poor thermostability and low catalytic efficiency limit its industrial saccharification applications....

    Authors: Lige Tong, Jie Zheng, Xiao Wang, Xiaolu Wang, Huoqing Huang, Haomeng Yang, Tao Tu, Yuan Wang, Yingguo Bai, Bin Yao, Huiying Luo and Xing Qin
    Citation: Biotechnology for Biofuels 2021 14:202
  29. The limited tolerance of Saccharomyces cerevisiae to inhibitors is a major challenge in second-generation bioethanol production, and our understanding of the molecular mechanisms providing tolerance to inhibitor-...

    Authors: Marlous van Dijk, Peter Rugbjerg, Yvonne Nygård and Lisbeth Olsson
    Citation: Biotechnology for Biofuels 2021 14:201
  30. Isobutanol is considered a potential biofuel, thanks to its high-energy content and octane value, limited water solubility, and compatibility with gasoline. As its biosynthesis pathway is known, a microorganis...

    Authors: Hsien-Lin Liu, Christine H.-T. Wang, En-Pei Isabel Chiang, Chieh-Chen Huang and Wen-Hsiung Li
    Citation: Biotechnology for Biofuels 2021 14:200
  31. Cellulase synthesized by fungi can environment-friendly and sustainably degrades cellulose to fermentable sugars for producing cellulosic biofuels, biobased medicine and fine chemicals. Great efforts have been...

    Authors: Ai-Ping Pang, Funing Zhang, Xin Hu, Yongsheng Luo, Haiyan Wang, Samran Durrani, Fu-Gen Wu, Bing-Zhi Li, Zhihua Zhou, Zuhong Lu and Fengming Lin
    Citation: Biotechnology for Biofuels 2021 14:199
  32. Lacto-N-triose II (LNT II), an important backbone for the synthesis of different human milk oligosaccharides, such as lacto-N-neotetraose and lacto-N-tetraose, has recently received significant attention. The pro...

    Authors: Duoduo Hu, Hao Wu, Yingying Zhu, Wenli Zhang and Wanmeng Mu
    Citation: Biotechnology for Biofuels 2021 14:198
  33. A platform for the utilization of the Chlorella sp. biomass and sugarcane leaves to produce multiple products (biorefinery concept) including hydrogen, methane, polyhydroxyalkanoates (PHAs), lipid, and soil suppl...

    Authors: Napapat Sitthikitpanya, Sureewan Sittijunda, Sontaya Khamtib and Alissara Reungsang
    Citation: Biotechnology for Biofuels 2021 14:197
  34. Tissue heterogeneity significantly influences the overall saccharification efficiency of plant biomass. However, the mechanisms of specific organ or tissue recalcitrance to enzymatic deconstruction are general...

    Authors: Yufen Wang, Xianyang Xu, Huiting Xue, Dejian Zhang and Guanhua Li
    Citation: Biotechnology for Biofuels 2021 14:196
  35. Xylanase, an important accessory enzyme that acts in synergy with cellulase, is widely used to degrade lignocellulosic biomass. Thermostable enzymes with good catalytic activity at lower temperatures have grea...

    Authors: Shuai You, Ziqian Zha, Jing Li, Wenxin Zhang, Zhiyuan Bai, Yanghao Hu, Xue Wang, Yiwen Chen, Zhongli Chen, Jun Wang and Huiying Luo
    Citation: Biotechnology for Biofuels 2021 14:195
  36. Sunflower stalk pith, residue from the processing of sunflower, is rich in pectin and cellulose, thereby acting as an economic raw material for the acquisition of these compounds. In order to increase the comm...

    Authors: Qibo Zhang, Lu Cheng, Xutong Ma, Xin Zhou and Yong Xu
    Citation: Biotechnology for Biofuels 2021 14:194
  37. Even if the loss of production capacity of a microorganism is said to be a serious problem in various biotechnology industries, reports in literature are rather rare. Strains of the genera Trichoderma reesei are ...

    Authors: R. Martzy, T. M. Mello-de-Sousa, R. L. Mach, D. Yaver and A. R. Mach-Aigner
    Citation: Biotechnology for Biofuels 2021 14:193
  38. Ethyl acetate (C4H8O2) and hydrogen (H2) are industrially relevant compounds that preferably are produced via sustainable, non-petrochemical production processes. Both compounds are volatile and can be produced b...

    Authors: Anna C. Bohnenkamp, René H. Wijffels, Servé W. M. Kengen and Ruud A. Weusthuis
    Citation: Biotechnology for Biofuels 2021 14:192
  39. Woody biomass has been considered as a promising feedstock for biofuel production via thermochemical conversion technologies such as fast pyrolysis. Extensive Life Cycle Assessment studies have been completed ...

    Authors: Kai Lan, Longwen Ou, Sunkyu Park, Stephen S. Kelley, Prakash Nepal, Hoyoung Kwon, Hao Cai and Yuan Yao
    Citation: Biotechnology for Biofuels 2021 14:191
  40. In the oilseed crop Brassica napus (rapeseed), various metabolic processes influence seed oil content, oil quality, and biological yield. However, the role of plastid membrane proteins in these traits has not bee...

    Authors: Zhongchun Xiao, Fang Tang, Liyuan Zhang, Shengting Li, Shufeng Wang, Qiang Huo, Bo Yang, Chao Zhang, Daojie Wang, Qing Li, Lijuan Wei, Tao Guo, Cunmin Qu, Kun Lu, Yanfeng Zhang, Liang Guo…
    Citation: Biotechnology for Biofuels 2021 14:190
  41. Lignin is a complex aromatic heteropolymer comprising 15–30% dry weight of the lignocellulose. The complex structural characteristic of lignin renders it difficult for value-added utilization. Exploring effici...

    Authors: Jiangshan Ma, Qiang Li, Yujie Wu, Huimin Yue, Yanghong Zhang, Jiashun Zhang, Muling Shi, Sixian Wang and Gao-Qiang Liu
    Citation: Biotechnology for Biofuels 2021 14:189
  42. Sugar alcohols are widely used as low-calorie sweeteners in the food and pharmaceutical industries. They can also be transformed into platform chemicals. Yarrowia lipolytica, an oleaginous yeast, is a promising h...

    Authors: Sujit Sadashiv Jagtap, Ashwini Ashok Bedekar, Vijay Singh, Yong-Su Jin and Christopher V. Rao
    Citation: Biotechnology for Biofuels 2021 14:188
  43. The industrial production of various alcohols from organic carbon compounds may be performed at high rates and with a low risk of contamination using thermophilic microorganisms as whole-cell catalysts. Thermoana...

    Authors: Lisa Hitschler, Laura Sofie Nissen, Michelle Kuntz and Mirko Basen
    Citation: Biotechnology for Biofuels 2021 14:187
  44. Consolidated bioprocessing (CBP) technique is a promising strategy for biorefinery construction, producing bulk chemicals directly from plant biomass without extra hydrolysis steps. Fixing and channeling CO2 into...

    Authors: Jingen Li, Bingchen Chen, Shuying Gu, Zhen Zhao, Qian Liu, Tao Sun, Yongli Zhang, Taju Wu, Defei Liu, Wenliang Sun and Chaoguang Tian
    Citation: Biotechnology for Biofuels 2021 14:186
  45. Recent research articles indicate that direct interspecies electron transfer (DIET) is an alternative metabolic route for methanogenic archaea that improves microbial methane productivity. It has been shown th...

    Authors: Kerstin Heitkamp, Adriel Latorre-Pérez, Sven Nefigmann, Helena Gimeno-Valero, Cristina Vilanova, Efri Jahmad and Christian Abendroth
    Citation: Biotechnology for Biofuels 2021 14:185
  46. The green microalga Chromochloris zofingiensis is capable of producing high levels of triacylglycerol rich in C18 unsaturated fatty acids (UFAs). FA desaturation degree is regulated by FA desaturases (FADs). Neve...

    Authors: Tao Wu, Lihua Yu, Yu Zhang and Jin Liu
    Citation: Biotechnology for Biofuels 2021 14:184
  47. Endo-β-1,4-galactanases are glycoside hydrolases (GH) from the GH53 family belonging to the largest clan of GHs, clan GH-A. GHs are ubiquitous and involved in a myriad of biological functions as well as being ...

    Authors: Sebastian J. Muderspach, Folmer Fredslund, Verena Volf, Jens-Christian Navarro Poulsen, Thomas H. Blicher, Mads Hartvig Clausen, Kim Krighaar Rasmussen, Kristian B. R. M. Krogh, Kenneth Jensen and Leila Lo Leggio
    Citation: Biotechnology for Biofuels 2021 14:183
  48. Bio-hydrogen production via dark fermentation of low-value waste is a potent and simple mean of recovering energy, maximising the harvesting of reducing equivalents to produce the cleanest fuel amongst renewab...

    Authors: M. Arizzi, S. Morra, G. Gilardi, M. Pugliese, M. L. Gullino and F. Valetti
    Citation: Biotechnology for Biofuels 2021 14:182
  49. Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates h...

    Authors: Zhi-Peng Wang, Xin-Yue Zhang, Yan Ma, Jing-Run Ye, Jing Jiang, Hai-Ying Wang and Wei Chen
    Citation: Biotechnology for Biofuels 2021 14:181