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  1. Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by Saccharomyces cerevisiae requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to...

    Authors: Rosa Garcia Sanchez, Kaisa Karhumaa, César Fonseca, Violeta Sànchez Nogué, João RM Almeida, Christer U Larsson, Oskar Bengtsson, Maurizio Bettiga, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2010 3:13

    Content type: Research

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  2. Corn grain is an important renewable source for bioethanol production in the USA. Corn ethanol is currently produced by steam liquefaction of starch-rich grains followed by enzymatic saccharification and ferme...

    Authors: Qianjun Shao, Shishir PS Chundawat, Chandraraj Krishnan, Bryan Bals, Leonardo da Costa Sousa, Kurt D Thelen, Bruce E Dale and Venkatesh Balan

    Citation: Biotechnology for Biofuels 2010 3:12

    Content type: Research

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  3. Fermentations using Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST), and Zymomonas mobilis AX101 are compared side-by-side on corn steep liquor (CSL) media and the water extract and enzymatic hydroly...

    Authors: Ming W Lau, Christa Gunawan, Venkatesh Balan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2010 3:11

    Content type: Research

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  4. Although measurements of crystallinity index (CI) have a long history, it has been found that CI varies significantly depending on the choice of measurement method. In this study, four different techniques inc...

    Authors: Sunkyu Park, John O Baker, Michael E Himmel, Philip A Parilla and David K Johnson

    Citation: Biotechnology for Biofuels 2010 3:10

    Content type: Research

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  5. Grasses are relatively recalcitrant to genetic transformation in comparison to certain dicotyledons, yet they constitute some of the most important biofuel crops. Genetic transformation of switchgrass (Panicum vi...

    Authors: Xinlu Chen, Raymie Equi, Holly Baxter, Kyle Berk, Jin Han, Sujata Agarwal and Janice Zale

    Citation: Biotechnology for Biofuels 2010 3:9

    Content type: Research

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  6. US legislation requires the use of advanced biofuels to be made from non-food feedstocks. However, commercialization of lignocellulosic ethanol technology is more complex than expected and is therefore running...

    Authors: NP Nghiem, KB Hicks, DB Johnston, G Senske, M Kurantz, M Li, J Shetty and G Konieczny-Janda

    Citation: Biotechnology for Biofuels 2010 3:8

    Content type: Research

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  7. The conditions for steam pretreatment of sugar cane bagasse and leaves were studied using CO2 as an impregnating agent. The following conditions were investigated: time (5 to 15 min) and temperature (190 to 220°C...

    Authors: Viridiana Ferreira-Leitão, Clarissa Cruz Perrone, Joice Rodrigues, Ana Paula Machado Franke, Stefano Macrelli and Guido Zacchi

    Citation: Biotechnology for Biofuels 2010 3:7

    Content type: Research

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  8. Different mechanistic models have been used in the literature to describe the enzymatic hydrolysis of pretreated biomass. Although these different models have been applied to different substrates, most of thes...

    Authors: Russell F Brown, Frank K Agbogbo and Mark T Holtzapple

    Citation: Biotechnology for Biofuels 2010 3:6

    Content type: Research

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  9. The enzymatic hydrolysis of cellulose is still considered as one of the main limiting steps of the biological production of biofuels from lignocellulosic biomass. It is a complex multistep process, and various...

    Authors: Marie Chauve, Hugues Mathis, Delphine Huc, Dominique Casanave, Frédéric Monot and Nicolas Lopes Ferreira

    Citation: Biotechnology for Biofuels 2010 3:3

    Content type: Research

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  10. Biofuels offer a viable alternative to petroleum-based fuel. However, current methods are not sufficient and the technology required in order to use lignocellulosic biomass as a fermentation substrate faces se...

    Authors: Sandra A Allen, William Clark, J Michael McCaffery, Zhen Cai, Alison Lanctot, Patricia J Slininger, Z Lewis Liu and Steven W Gorsich

    Citation: Biotechnology for Biofuels 2010 3:2

    Content type: Research

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  11. When producing biofuels from dedicated feedstock, agronomic factors such as harvest time and location can impact the downstream production. Thus, this paper studies the effectiveness of ammonia fibre expansion...

    Authors: Bryan Bals, Chad Rogers, Mingjie Jin, Venkatesh Balan and Bruce Dale

    Citation: Biotechnology for Biofuels 2010 3:1

    Content type: Research

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  12. In this study, the dilute maleic acid pretreatment of wheat straw is optimized, using pretreatment time, temperature and maleic acid concentration as design variables. A central composite design was applied to...

    Authors: A Maarten J Kootstra, Hendrik H Beeftink, Elinor L Scott and Johan PM Sanders

    Citation: Biotechnology for Biofuels 2009 2:31

    Content type: Research

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  13. Pretreatment chemistry is of central importance due to its impacts on cellulosic biomass processing and biofuels conversion. Ammonia fiber expansion (AFEX) and dilute acid are two promising pretreatments using...

    Authors: Ming W Lau, Christa Gunawan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2009 2:30

    Content type: Research

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  14. Corn stover composition changes considerably throughout the growing season and also varies between the various fractions of the plant. These differences can impact optimal pretreatment conditions, enzymatic di...

    Authors: Rebecca J Garlock, Shishir PS Chundawat, Venkatesh Balan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2009 2:29

    Content type: Research

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  15. Screening new lignocellulosic biomass pretreatments and advanced enzyme systems at process relevant conditions is a key factor in the development of economically viable lignocellulosic ethanol. Shake flasks, t...

    Authors: Christine M Roche, Clare J Dibble and Jonathan J Stickel

    Citation: Biotechnology for Biofuels 2009 2:28

    Content type: Research

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  16. Termite lignocellulose digestion is achieved through a collaboration of host plus prokaryotic and eukaryotic symbionts. In the present work, we took a combined host and symbiont metatranscriptomic approach for...

    Authors: Aurélien Tartar, Marsha M Wheeler, Xuguo Zhou, Monique R Coy, Drion G Boucias and Michael E Scharf

    Citation: Biotechnology for Biofuels 2009 2:25

    Content type: Research

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  17. Bioethanol isolated from lignocellulosic biomass represents one of the most promising renewable and carbon neutral alternative liquid fuel sources. Enzymatic saccharification using cellulase has proven to be a...

    Authors: Tatsuya Fujii, Xu Fang, Hiroyuki Inoue, Katsuji Murakami and Shigeki Sawayama

    Citation: Biotechnology for Biofuels 2009 2:24

    Content type: Research

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  18. Mary E H Mandels, who spearheaded the US Army's national bioconversion studies for four decades and was an early proponent of conversion of waste biomass to readily bioconvertible sugars for the production of ...

    Authors: Fred Allen, Raymond Andreotti, Douglas E Eveleigh and John Nystrom

    Citation: Biotechnology for Biofuels 2009 2:22

    Content type: Commentary

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  19. This article sets forth a simple cellulase assay procedure. Cellulose is variable in nature, insoluble and resistant to enzymatic attack. As a result there have been a bevy of bewildering cellulase assays publ...

    Authors: Douglas E Eveleigh, Mary Mandels, Raymond Andreotti and Charles Roche

    Citation: Biotechnology for Biofuels 2009 2:21

    Content type: Commentary

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  20. This article, 'Reflections on the United States Military 1941-1987' written by my grandmother, Mary Mandels, illustrates her passion for life. Her outreach article was considered most appropriate for publicati...

    Authors: Mary Mandels and Douglas E Eveleigh

    Citation: Biotechnology for Biofuels 2009 2:20

    Content type: Commentary

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  21. Hypocrea jecorina (= Trichoderma reesei) is the main industrial source of cellulases and hemicellulases used to depolymerise plant biomass to simple sugars that are converted to chemical intermediates and biofuel...

    Authors: Christian P Kubicek, Marianna Mikus, André Schuster, Monika Schmoll and Bernhard Seiboth

    Citation: Biotechnology for Biofuels 2009 2:19

    Content type: Review

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  22. Two economic factors make watermelon worthy of consideration as a feedstock for ethanol biofuel production. First, about 20% of each annual watermelon crop is left in the field because of surface blemishes or ...

    Authors: Wayne W Fish, Benny D Bruton and Vincent M Russo

    Citation: Biotechnology for Biofuels 2009 2:18

    Content type: Research

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  23. Drying is currently the most frequently used conservation method for cereal grain, which in temperate climates consumes a major part of process energy. Airtight storage of moist feed grain using the biocontrol...

    Authors: Volkmar Passoth, Anna Eriksson, Mats Sandgren, Jerry Ståhlberg, Kathleen Piens and Johan Schnürer

    Citation: Biotechnology for Biofuels 2009 2:16

    Content type: Research

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  24. Improvement of the process of cellulase production and development of more efficient lignocellulose-degrading enzymes are necessary in order to reduce the cost of enzymes required in the biomass-to-bioethanol ...

    Authors: Krisztina Kovacs, Stefano Macrelli, George Szakacs and Guido Zacchi

    Citation: Biotechnology for Biofuels 2009 2:14

    Content type: Research

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  25. This work explores the potential contribution of bioenergy technologies to 60% and 80% carbon reductions in the UK energy system by 2050, by outlining the potential for accelerated technological development of...

    Authors: Donna Clarke, Sophie Jablonski, Brighid Moran, Gabrial Anandarajah and Gail Taylor

    Citation: Biotechnology for Biofuels 2009 2:13

    Content type: Research

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  26. The production of hydrogen from biomass by fermentation is one of the routes that can contribute to a future sustainable hydrogen economy. Lignocellulosic biomass is an attractive feedstock because of its abun...

    Authors: Truus de Vrije, Robert R Bakker, Miriam AW Budde, Man H Lai, Astrid E Mars and Pieternel AM Claassen

    Citation: Biotechnology for Biofuels 2009 2:12

    Content type: Research

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  27. Throughout immeasurable time, microorganisms evolved and accumulated remarkable physiological and functional heterogeneity, and now constitute the major reserve for genetic diversity on earth. Using metagenomi...

    Authors: Luen-Luen Li, Sean R McCorkle, Sebastien Monchy, Safiyh Taghavi and Daniel van der Lelie

    Citation: Biotechnology for Biofuels 2009 2:10

    Content type: Review

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  28. Xylose reductase (XR) and xylitol dehydrogenase (XDH) from Pichia stipitis are the two enzymes most commonly used in recombinant Saccharomyces cerevisiae strains engineered for xylose utilization. The availabilit...

    Authors: Oskar Bengtsson, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2009 2:9

    Content type: Research

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  29. Simultaneous saccharification and fermentation (SSF) is a promising process option for ethanol production from lignocellulosic materials. However, both the overall ethanol yield and the final ethanol concentra...

    Authors: Magnus Bertilsson, Kim Olofsson and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2009 2:8

    Content type: Research

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  30. Microbial fuel cells (MFC) and microbial electrolysis cells are electrical devices that treat water using microorganisms and convert soluble organic matter into electricity and hydrogen, respectively. Emerging...

    Authors: Abhijeet P Borole, Jonathan R Mielenz, Tatiana A Vishnivetskaya and Choo Y Hamilton

    Citation: Biotechnology for Biofuels 2009 2:7

    Content type: Research

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  31. Two-step dilute acid hydrolysis of softwood, either as a stand-alone process or as pretreatment before enzymatic hydrolysis, is considered to result in higher sugar yields than one-step acid hydrolysis. Howeve...

    Authors: Sanam Monavari, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2009 2:6

    Content type: Research

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  32. Improvement of biofeedstock quality for cellulosic ethanol production will be facilitated by inexpensive and rapid methods of evaluation, such as those already employed in the field of ruminant nutrition. Our ...

    Authors: Aaron J Lorenz, Rob P Anex, Asli Isci, James G Coors, Natalia de Leon and Paul J Weimer

    Citation: Biotechnology for Biofuels 2009 2:5

    Content type: Research

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  33. Enzymatic corn wet milling (E-milling) is a process derived from conventional wet milling for the recovery and purification of starch and co-products using proteases to eliminate the need for sulfites and decr...

    Authors: Edna C Ramírez, David B Johnston, Andrew J McAloon and Vijay Singh

    Citation: Biotechnology for Biofuels 2009 2:2

    Content type: Research

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  34. Transesterification of Jatropha oil was carried out in t-butanol solvent using immobilized lipase from Enterobacter aerogenes. The presence of t-butanol significantly reduced the negative effects caused by both m...

    Authors: Annapurna Kumari, Paramita Mahapatra, Vijay Kumar Garlapati and Rintu Banerjee

    Citation: Biotechnology for Biofuels 2009 2:1

    Content type: Research

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  35. Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic bioma...

    Authors: Isabelle Herpoël-Gimbert, Antoine Margeot, Alain Dolla, Gwénaël Jan, Daniel Mollé, Sabrina Lignon, Hughes Mathis, Jean-Claude Sigoillot, Frédéric Monot and Marcel Asther

    Citation: Biotechnology for Biofuels 2008 1:18

    Content type: Research

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  36. The availability and low cost of lignocellulosic biomass has caused tremendous interest in the bioconversion of this feedstock into liquid fuels. One measure of the economic viability of the bioconversion proc...

    Authors: Lisa J Haney, James G Coors, Aaron J Lorenz, DRaj Raman, Robert P Anex and MPaul Scott

    Citation: Biotechnology for Biofuels 2008 1:17

    Content type: Research

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  37. Ethanolic fermentation of lignocellulosic biomass is a sustainable option for the production of bioethanol. This process would greatly benefit from recombinant Saccharomyces cerevisiae strains also able to fermen...

    Authors: Maurizio Bettiga, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2008 1:16

    Content type: Research

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  38. Brachypodium distachyon constitutes an excellent model species for grasses. It is a small, easily propagated, temperate grass with a rapid life cycle and a small genome. It is a self-fertile plant that can be tra...

    Authors: Leonardo D Gomez, Jennifer K Bristow, Emily R Statham and Simon J McQueen-Mason

    Citation: Biotechnology for Biofuels 2008 1:15

    Content type: Research

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  39. The limited availability of fossil fuel sources, worldwide rising energy demands and anticipated climate changes attributed to an increase of greenhouse gasses are important driving forces for finding alternat...

    Authors: Ronald HW Maas, Robert R Bakker, Arjen R Boersma, Iemke Bisschops, Jan R Pels, Ed de Jong, Ruud A Weusthuis and Hans Reith

    Citation: Biotechnology for Biofuels 2008 1:14

    Content type: Research

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  40. Lignocellulosic bioethanol technologies exhibit significant capacity for performance improvement across the supply chain through the development of high-yielding energy crops, integrated pretreatment, hydrolys...

    Authors: Alex J Dunnett, Claire S Adjiman and Nilay Shah

    Citation: Biotechnology for Biofuels 2008 1:13

    Content type: Methodology

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