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  1. Lignocellulosic biomass will progressively become the main source of carbon for a number of products as the Earth’s oil reservoirs disappear. Technology for conversion of wood fiber into bioproducts (wood bior...

    Authors: Pierre-Louis Bombeck, Vinay Khatri, Fatma Meddeb-Mouelhi, Daniel Montplaisir, Aurore Richel and Marc Beauregard
    Citation: Biotechnology for Biofuels 2017 10:293
    Content type: Research Published on:

  3. The efficiency of cellulolytic enzymes is important in industrial biorefinery processes, including biofuel production. Chemical methods, such as alkali pretreatment, have been extensively studied and demonstra...

    Authors: Kiyota Sakai, Saki Kojiya, Junya Kamijo, Yuta Tanaka, Kenta Tanaka, Masahiro Maebayashi, Jun-Seok Oh, Masafumi Ito, Masaru Hori, Motoyuki Shimizu and Masashi Kato
    Citation: Biotechnology for Biofuels 2017 10:290
    Content type: Research Published on:

  4. High temperature inhibits cell growth and ethanol fermentation of Saccharomyces cerevisiae. As a complex phenotype, thermotolerance usually involves synergistic actions of many genes, thereby being difficult to e...

    Authors: Pengsong Li, Xiaofen Fu, Lei Zhang, Zhiyu Zhang, Jihong Li and Shizhong Li
    Citation: Biotechnology for Biofuels 2017 10:289
    Content type: Research Published on:

  5. Tobacco is widely planted as an important nonfood economic crop throughout the world, and large amounts of tobacco wastes are generated during the tobacco manufacturing process. Tobacco and its wastes contain ...

    Authors: Wenjun Yu, Rongshui Wang, Huili Li, Jiyu Liang, Yuanyuan Wang, Haiyan Huang, Huijun Xie and Shuning Wang
    Citation: Biotechnology for Biofuels 2017 10:288
    Content type: Research Published on:

  6. The authors noticed an accidental calculation error existing in Fig. 4C of this article [1]. The given velocities of the GAT-1 transporter (in this figure only) were mistakenly listed 50-fold too high. Based on t...

    Authors: J. Philipp Benz, Ryan J. Protzko, Jonas M. S. Andrich, Stefan Bauer, John E. Dueber and Chris R. Somerville
    Citation: Biotechnology for Biofuels 2017 10:287
    Content type: Correction Published on:

    The original article was published in Biotechnology for Biofuels 2014 7:20

  7. Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from na...

    Authors: Vanessa A. Thomas, Ninad Kothari, Samarthya Bhagia, Hannah Akinosho, Mi Li, Yunqiao Pu, Chang Geun Yoo, Sivakumar Pattathil, Michael G. Hahn, Arthur J. Raguaskas, Charles E. Wyman and Rajeev Kumar
    Citation: Biotechnology for Biofuels 2017 10:292
    Content type: Research Published on:

  8. Xylans are the most abundant noncellulosic polysaccharides in lignified secondary cell walls of woody dicots and in both primary and secondary cell walls of grasses. These polysaccharides, which comprise 20–35...

    Authors: Peter J. Smith, Hsin-Tzu Wang, William S. York, Maria J. Peña and Breeanna R. Urbanowicz
    Citation: Biotechnology for Biofuels 2017 10:286
    Content type: Review Published on:

  9. The DOE BioEnergy Science Center has operated as a virtual center with multiple partners for a decade targeting overcoming biomass recalcitrance. BESC has redefined biomass recalcitrance from an observable phe...

    Authors: Paul Gilna, Lee R. Lynd, Debra Mohnen, Mark F. Davis and Brian H. Davison
    Citation: Biotechnology for Biofuels 2017 10:285
    Content type: Commentary Published on:

  10. The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with an allotetraploid genome (2n = 4x =...

    Authors: Jong-Jin Park, Chang Geun Yoo, Amy Flanagan, Yunqiao Pu, Smriti Debnath, Yaxin Ge, Arthur J. Ragauskas and Zeng-Yu Wang
    Citation: Biotechnology for Biofuels 2017 10:284
    Content type: Research Published on:

  11. With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20–31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenti...

    Authors: Ayşenur Eminoğlu, Sean Jean-Loup Murphy, Marybeth Maloney, Anthony Lanahan, Richard J. Giannone, Robert L. Hettich, Shital A. Tripathi, Ali Osman Beldüz, Lee R. Lynd and Daniel G. Olson
    Citation: Biotechnology for Biofuels 2017 10:282
    Content type: Research Published on:

  13. Clostridium thermocellum is a promising microorganism for conversion of cellulosic biomass to biofuel, without added enzymes; however, the low ethanol titer produced by strains develop...

    Authors: Liang Tian, Skyler J. Perot, David Stevenson, Tyler Jacobson, Anthony A. Lanahan, Daniel Amador-Noguez, Daniel G. Olson and Lee R. Lynd
    Citation: Biotechnology for Biofuels 2017 10:276
    Content type: Research Published on:

  14. Glycoside hydrolase (GH) family 48 is an understudied and increasingly important exoglucanase family found in the majority of bacterial cellulase systems. Moreover, many thermophilic enzyme systems contain GH4...

    Authors: Roman Brunecky, Markus Alahuhta, Deanne W. Sammond, Qi Xu, Mo Chen, David B. Wilson, John W. Brady, Michael E. Himmel, Yannick J. Bomble and Vladimir V. Lunin
    Citation: Biotechnology for Biofuels 2017 10:274
    Content type: Research Published on:

  15. Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are prominent candidate biocatalysts that, together, can enable the direct biotic conversion of lignocellulosic bioma...

    Authors: Kyle Sander, Keiji G. Asano, Deepak Bhandari, Gary J. Van Berkel, Steven D. Brown, Brian Davison and Timothy J. Tschaplinski
    Citation: Biotechnology for Biofuels 2017 10:270
    Content type: Methodology Published on:

  16. Plant cell walls contribute the majority of plant biomass that can be used to produce transportation fuels. However, the complexity and variability in composition and structure of cell walls, particularly the ...

    Authors: Xiaolan Rao, Hui Shen, Sivakumar Pattathil, Michael G. Hahn, Ivana Gelineo-Albersheim, Debra Mohnen, Yunqiao Pu, Arthur J. Ragauskas, Xin Chen, Fang Chen and Richard A. Dixon
    Citation: Biotechnology for Biofuels 2017 10:266
    Content type: Research Published on:

  17. Understanding plant cell wall cross-linking chemistry and polymeric architecture is key to the efficient utilization of biomass in all prospects from rational genetic modification to downstream chemical and bi...

    Authors: Yining Zeng, Michael E. Himmel and Shi-You Ding
    Citation: Biotechnology for Biofuels 2017 10:263
    Content type: Review Published on:

  19. Genetic engineering has been effective in altering cell walls for biofuel production in the bioenergy crop, switchgrass (Panicum virgatum). However, regulatory issues arising from gene flow may prevent commercial...

    Authors: Chelsea R. Johnson, Reginald J. Millwood, Yuhong Tang, Jiqing Gou, Robert W. Sykes, Geoffrey B. Turner, Mark F. Davis, Yi Sang, Zeng-Yu Wang and C. Neal Stewart Jr.
    Citation: Biotechnology for Biofuels 2017 10:255
    Content type: Research Published on:

  20. One of the major barriers to the development of lignocellulosic feedstocks is the recalcitrance of plant cell walls to deconstruction and saccharification. Recalcitrance can be reduced by targeting genes invol...

    Authors: David Macaya-Sanz, Jin‐Gui Chen, Udaya C. Kalluri, Wellington Muchero, Timothy J. Tschaplinski, Lee E. Gunter, Sandra J. Simon, Ajaya K. Biswal, Anthony C. Bryan, Raja Payyavula, Meng Xie, Yongil Yang, Jin Zhang, Debra Mohnen, Gerald A. Tuskan and Stephen P. DiFazio
    Citation: Biotechnology for Biofuels 2017 10:253
    Content type: Research Published on:

  21. Consolidated bioprocessing (CBP) by anaerobes, such as Clostridium thermocellum, which combine enzyme production, hydrolysis, and fermentation are promising alternatives to historical economic challenges of using...

    Authors: Vanessa A. Thomas, Bryon S. Donohoe, Mi Li, Yunqiao Pu, Arthur J. Ragauskas, Rajeev Kumar, Thanh Yen Nguyen, Charles M. Cai and Charles E. Wyman
    Citation: Biotechnology for Biofuels 2017 10:252
    Content type: Research Published on:

  23. Xylan is a major hemicellulosic component in the cell walls of higher plants especially in the secondary walls of vascular cells which are playing important roles in physiological processes and overall mechani...

    Authors: Angelo G. Peralta, Sivasankari Venkatachalam, Sydney C. Stone and Sivakumar Pattathil
    Citation: Biotechnology for Biofuels 2017 10:245
    Content type: Research Published on:

  24. Auxiliary activity (AA) enzymes are produced by numerous bacterial and fungal species to assist in the degradation of biomass. These enzymes are abundant but have yet to be fully characterized. Here, we report...

    Authors: Nathan Kruer-Zerhusen, Markus Alahuhta, Vladimir V. Lunin, Michael E. Himmel, Yannick J. Bomble and David B. Wilson
    Citation: Biotechnology for Biofuels 2017 10:243
    Content type: Research Published on:

  25. Hydrothermal pretreatment using liquid hot water (LHW) is capable of substantially reducing the cell wall recalcitrance of lignocellulosic biomass. It enhances the saccharification of polysaccharides, particul...

    Authors: Mi Li, Shilin Cao, Xianzhi Meng, Michael Studer, Charles E. Wyman, Arthur J. Ragauskas and Yunqiao Pu
    Citation: Biotechnology for Biofuels 2017 10:237
    Content type: Research Published on:

  26. Efficient deconstruction and bioconversion of solids at high mass loadings is necessary to produce industrially relevant titers of biofuels from lignocellulosic biomass. To date, only a few studies have invest...

    Authors: Tobin J. Verbeke, Gabriela M. Garcia and James G. Elkins
    Citation: Biotechnology for Biofuels 2017 10:233
    Content type: Research Published on:

  27. Genetically engineered biofuel crops, such as switchgrass (Panicum virgatum L.), that produce their own cell wall-digesting cellulase enzymes would reduce costs of cellulosic biofuel production. To date, non-bioe...

    Authors: Jonathan D. Willis, Joshua N. Grant, Mitra Mazarei, Lindsey M. Kline, Caroline S. Rempe, A. Grace Collins, Geoffrey B. Turner, Stephen R. Decker, Robert W. Sykes, Mark F. Davis, Nicole Labbe, Juan L. Jurat-Fuentes and C. Neal Stewart Jr.
    Citation: Biotechnology for Biofuels 2017 10:230
    Content type: Research Published on:

  28. In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel...

    Authors: Michael E. Himmel, Charles A. Abbas, John O. Baker, Edward A. Bayer, Yannick J. Bomble, Roman Brunecky, Xiaowen Chen, Claus Felby, Tina Jeoh, Rajeev Kumar, Barry V. McCleary, Brett I. Pletschke, Melvin P. Tucker, Charles E. Wyman and Stephen R. Decker
    Citation: Biotechnology for Biofuels 2017 10:283
    Content type: Commentary Published on:

  29. Nitrogen deprivation and replenishment induces massive changes at the physiological and molecular level in the green alga Chlamydomonas reinhardtii, including reversible starch and lipid accumulation. Stress sign...

    Authors: Valentin Roustan, Shiva Bakhtiari, Pierre-Jean Roustan and Wolfram Weckwerth
    Citation: Biotechnology for Biofuels 2017 10:280
    Content type: Research Published on:

  30. The transition to a more environmentally friendly economy has prompted studies of modern biorefineries, including the utilization of low-value lignocellulose. The major challenge facing the widespread applicat...

    Authors: Yu-Si Yan, Shuai Zhao, Lu-Sheng Liao, Qi-Peng He, Ya-Ru Xiong, Long Wang, Cheng-Xi Li and Jia-Xun Feng
    Citation: Biotechnology for Biofuels 2017 10:279
    Content type: Research Published on:

  31. Trichoderma reesei is considered a candidate fungal enzyme producer for the economic saccharification of cellulosic biomass. However, performance of the saccharifying enzymes produced ...

    Authors: Nozomu Shibata, Mari Suetsugu, Hiroshi Kakeshita, Kazuaki Igarashi, Hiroshi Hagihara and Yasushi Takimura
    Citation: Biotechnology for Biofuels 2017 10:278
    Content type: Research Published on:

  32. A future bioeconomy relies on the efficient use of renewable resources for energy and material product supply. In this context, biorefineries have been developed and play a key role in converting lignocellulos...

    Authors: Julian Lange, Felix Müller, Kerstin Bernecker, Nicolaus Dahmen, Ralf Takors and Bastian Blombach
    Citation: Biotechnology for Biofuels 2017 10:277
    Content type: Research Published on:

  33. Current approaches for quantification of major energy-storage forms in microalgae, including starch, protein and lipids, generally require cell cultivation to collect biomass followed by tedious and time-consu...

    Authors: Yuehui He, Peng Zhang, Shi Huang, Tingting Wang, Yuetong Ji and Jian Xu
    Citation: Biotechnology for Biofuels 2017 10:275
    Content type: Methodology Published on:

  34. Synechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found to be 99.8% identical to S. elongatus 7942 yet it grows twice as fas...

    Authors: Mary H. Abernathy, Jingjie Yu, Fangfang Ma, Michelle Liberton, Justin Ungerer, Whitney D. Hollinshead, Saratram Gopalakrishnan, Lian He, Costas D. Maranas, Himadri B. Pakrasi, Doug K. Allen and Yinjie J. Tang
    Citation: Biotechnology for Biofuels 2017 10:273
    Content type: Research Published on:

  35. The enzymes for efficient hydrolysis of lignocellulosic biomass are a major factor in the development of an economically feasible cellulose bioconversion process. Up to now, low hydrolysis efficiency and high ...

    Authors: Jia Gao, Yuanchao Qian, Yifan Wang, Yinbo Qu and Yaohua Zhong
    Citation: Biotechnology for Biofuels 2017 10:272
    Content type: Research Published on:

  36. Lignocellulosic biomass is an important resource for renewable production of biofuels and bioproducts. Enzymes that deconstruct this biomass are critical for the viability of biomass-based biofuel production p...

    Authors: Timo Schuerg, Jan-Philip Prahl, Raphael Gabriel, Simon Harth, Firehiwot Tachea, Chyi-Shin Chen, Matthew Miller, Fabrice Masson, Qian He, Sarah Brown, Mona Mirshiaghi, Ling Liang, Lauren M. Tom, Deepti Tanjore, Ning Sun, Todd R. Pray…
    Citation: Biotechnology for Biofuels 2017 10:271
    Content type: Research Published on:

  37. The Bacillus subtilis endo-β-1,4-glucanase (BsCel5A) hydrolyzes β-1,3-1,4-linked glucan, and the enzyme includes a family 3 carbohydrate-binding module (CBM3) that binds β-1,4-linked glucan.

    Authors: Raquel Fonseca-Maldonado, Luana P. Meleiro, Luís F. S. Mendes, Luana F. Alves, Sibeli Carli, Lucas D. Morero, Luis G. M. Basso, Antonio J. Costa-Filho and Richard J. Ward
    Citation: Biotechnology for Biofuels 2017 10:269
    Content type: Research Published on:

  38. The need for liquid fuels in the transportation sector is increasing, and it is essential to develop industrially sustainable processes that simultaneously address the tri-fold sustainability metrics of techno...

    Authors: Karthik Rajendran and Ganti S. Murthy
    Citation: Biotechnology for Biofuels 2017 10:268
    Content type: Research Published on:

  39. Genome editing techniques are critical for manipulating genes not only to investigate their functions in biology but also to improve traits for genetic engineering in biotechnology. Genome editing has been gre...

    Authors: Seungjib Jeon, Jong-Min Lim, Hyung-Gwan Lee, Sung-Eun Shin, Nam Kyu Kang, Youn-Il Park, Hee-Mock Oh, Won-Joong Jeong, Byeong-ryool Jeong and Yong Keun Chang
    Citation: Biotechnology for Biofuels 2017 10:267
    Content type: Review Published on:

  40. Genome and transcriptome sequencing has greatly facilitated the understanding of biomass-degrading mechanisms in a number of fungal species. The information obtained enables the investigation and discovery of ...

    Authors: Silvia Hüttner, Thanh Thuy Nguyen, Zoraide Granchi, Thomas Chin-A-Woeng, Dag Ahrén, Johan Larsbrink, Vu Nguyen Thanh and Lisbeth Olsson
    Citation: Biotechnology for Biofuels 2017 10:265
    Content type: Research Published on:

  41. To elucidate biogas microbial communities and processes, the application of high-throughput DNA analysis approaches is becoming increasingly important. Unfortunately, generated data can only partialy be interp...

    Authors: Irena Maus, Andreas Bremges, Yvonne Stolze, Sarah Hahnke, Katharina G. Cibis, Daniela E. Koeck, Yong S. Kim, Jana Kreubel, Julia Hassa, Daniel Wibberg, Aaron Weimann, Sandra Off, Robbin Stantscheff, Vladimir V. Zverlov, Wolfgang H. Schwarz, Helmut König…
    Citation: Biotechnology for Biofuels 2017 10:264
    Content type: Research Published on:

  43. The light/dark cycle is one of the most important factors affecting the microalgal growth and lipid accumulation. Biomass concentration and lipid productivity could be enhanced by optimization of light/dark cy...

    Authors: Chao Ma, Yan-Bo Zhang, Shih-Hsin Ho, De-Feng Xing, Nan-Qi Ren and Bing-Feng Liu
    Citation: Biotechnology for Biofuels 2017 10:260
    Content type: Research Published on:

  44. Pig manure utilization and valorization is an important topic with tightening regulations focused on ecological and safety issues. By itself pig manure is a poor substrate for biogas production because of its ...

    Authors: Michał Gaworski, Sławomir Jabłoński, Izabela Pawlaczyk-Graja, Rafał Ziewiecki, Piotr Rutkowski, Anna Wieczyńska, Roman Gancarz and Marcin Łukaszewicz
    Citation: Biotechnology for Biofuels 2017 10:259
    Content type: Research Published on:

  46. Chlamydomonas reinhardtii is an ideal model organism not only for the study of basic metabolic processes in both plants and animals but also the production of biofuels including hydrog...

    Authors: Yuting Wang, Xinqin Jiang, Changxing Hu, Ting Sun, Zhiyong Zeng, Xiaoqi Cai, Hui Li and Zhangli Hu
    Citation: Biotechnology for Biofuels 2017 10:257
    Content type: Research Published on:

  47. Cellulose, the most abundant biopolymer on earth, is an alternative for fossil fuels as a renewable feedstock for the production of second-generation biofuels and other chemicals. The discovery of novel, highl...

    Authors: J. A. Méndez-Líter, J. Gil-Muñoz, M. Nieto-Domínguez, J. Barriuso, L. I. de Eugenio and M. J. Martínez
    Citation: Biotechnology for Biofuels 2017 10:256
    Content type: Research Published on:

  48. Using globally abundant crop residues as a carbon source for energy generation and renewable chemicals production stand out as a promising solution to reduce current dependency on fossil fuels. In nature, such...

    Authors: Bruno L. Mello, Anna M. Alessi, Diego M. Riaño-Pachón, Eduardo R. deAzevedo, Francisco E. G. Guimarães, Melissa C. Espirito Santo, Simon McQueen-Mason, Neil C. Bruce and Igor Polikarpov
    Citation: Biotechnology for Biofuels 2017 10:254
    Content type: Research Published on:

  49. Like a number of anaerobic and cellulolytic Gram-positive bacteria, the model microorganism Ruminiclostridium cellulolyticum produces extracellular multi-enzymatic complexes called cellulosomes, which efficiently...

    Authors: Aurélie Fosses, Maria Maté, Nathalie Franche, Nian Liu, Yann Denis, Romain Borne, Pascale de Philip, Henri-Pierre Fierobe and Stéphanie Perret
    Citation: Biotechnology for Biofuels 2017 10:250
    Content type: Research Published on:

  50. Enterobacter aerogenes is a facultative anaerobe and is one of the most widely studied bacterial strains because of its ability to use a variety of substrates, to produce hydrogen at a...

    Authors: Yan Wu, Yaqiao Hao, Xuan Wei, Qi Shen, Xuanwei Ding, Liyan Wang, Hongxin Zhao and Yuan Lu
    Citation: Biotechnology for Biofuels 2017 10:248
    Content type: Research Published on:
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