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  1. Content type: Research

    High enzyme loading is a major economic bottleneck for the commercial processing of pretreated lignocellulosic biomass to produce fermentable sugars. Optimizing the enzyme cocktail for specific types of pretre...

    Authors: Dahai Gao, Nirmal Uppugundla, Shishir PS Chundawat, Xiurong Yu, Spencer Hermanson, Krishne Gowda, Phillip Brumm, David Mead, Venkatesh Balan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2011 4:5

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  2. Content type: Research

    The discovery and development of novel plant cell wall degrading enzymes is a key step towards more efficient depolymerization of polysaccharides to fermentable sugars for the production of liquid transportati...

    Authors: Brian C King, Katrina D Waxman, Nicholas V Nenni, Larry P Walker, Gary C Bergstrom and Donna M Gibson

    Citation: Biotechnology for Biofuels 2011 4:4

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  3. Content type: Research

    A range of lignocellulosic feedstocks (including agricultural, softwood and hardwood substrates) were pretreated with either sulfur dioxide-catalyzed steam or an ethanol organosolv procedure to try to establis...

    Authors: Valdeir Arantes and Jack N Saddler

    Citation: Biotechnology for Biofuels 2011 4:3

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  4. Content type: Research

    Thermostable enzymes have several benefits in lignocellulose processing. In particular, they potentially allow the use of increased substrate concentrations (because the substrate viscosity decreases as the te...

    Authors: Nóra Szijártó, Emma Horan, Junhua Zhang, Terhi Puranen, Matti Siika-aho and Liisa Viikari

    Citation: Biotechnology for Biofuels 2011 4:2

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  5. Content type: Research

    The glycoside hydrolase family 5 endocellulase, E1 (Cel5A), from Acidothermus cellulolyticus was transformed into both Nicotiana tabacum and Zea mays with expression targeted to the cell wall under a constitutive...

    Authors: Roman Brunecky, Michael J Selig, Todd B Vinzant, Michael E Himmel, David Lee, Michael J Blaylock and Stephen R Decker

    Citation: Biotechnology for Biofuels 2011 4:1

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  6. Content type: Research

    Lignin is embedded in the plant cell wall matrix, and impedes the enzymatic saccharification of lignocellulosic feedstocks. To investigate whether enzymatic digestibility of cell wall materials can be improved...

    Authors: Xu Li, Eduardo Ximenes, Youngmi Kim, Mary Slininger, Richard Meilan, Michael Ladisch and Clint Chapple

    Citation: Biotechnology for Biofuels 2010 3:27

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  7. Content type: Research

    Degradation of the toxic compounds generated in the harsh pretreatment of lignocellulose is an inevitable step in reducing the toxin level for conducting practical enzymatic hydrolysis and ethanol fermentation...

    Authors: Jian Zhang, Zhinan Zhu, Xiaofeng Wang, Nan Wang, Wei Wang and Jie Bao

    Citation: Biotechnology for Biofuels 2010 3:26

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  8. Content type: Research

    Variations in sugar yield due to genotypic qualities of feedstock are largely undescribed for pilot-scale ethanol processing. Our objectives were to compare glucose and xylose yield (conversion and total sugar...

    Authors: Jane Lindedam, Sander Bruun, Henning Jørgensen, Claus Felby and Jakob Magid

    Citation: Biotechnology for Biofuels 2010 3:25

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  9. Content type: Research

    Cell wall resistance represents the main barrier for the production of second generation biofuels. The deconstruction of lignocellulose can provide sugars for the production of fuels or other industrial produc...

    Authors: Leonardo D Gomez, Caragh Whitehead, Abdellah Barakate, Claire Halpin and Simon J McQueen-Mason

    Citation: Biotechnology for Biofuels 2010 3:23

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  10. Content type: Research

    Enzymes for plant cell wall deconstruction are a major cost in the production of ethanol from lignocellulosic biomass. The goal of this research was to develop optimized synthetic mixtures of enzymes for multi...

    Authors: Goutami Banerjee, Suzana Car, John S Scott-Craig, Melissa S Borrusch and Jonathan D Walton

    Citation: Biotechnology for Biofuels 2010 3:22

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  11. Content type: Research

    The complex technology of converting lignocellulose to fuels such as ethanol has advanced rapidly over the past few years, and enzymes are a critical component of this technology. The production of effective e...

    Authors: Suzanne E Lantz, Frits Goedegebuur, Ronald Hommes, Thijs Kaper, Bradley R Kelemen, Colin Mitchinson, Louise Wallace, Jerry Ståhlberg and Edmundo A Larenas

    Citation: Biotechnology for Biofuels 2010 3:20

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  12. Content type: Research

    Overexpression of the PGM2 gene encoding phosphoglucomutase (Pgm2p) has been shown to improve galactose utilization both under aerobic and under anaerobic conditions. Similarly, xylose utilization has been improv...

    Authors: Rosa Garcia Sanchez, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2010 3:19

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  13. Content type: Research

    It is important to generate biofuels and society must be weaned from its dependency on fossil fuels. In order to produce biofuels, lignocellulose is pretreated and the resulting cellulose is hydrolyzed by cell...

    Authors: Gernot Jäger, Zhuojun Wu, Kerstin Garschhammer, Philip Engel, Tobias Klement, Roberto Rinaldi, Antje C Spiess and Jochen Büchs

    Citation: Biotechnology for Biofuels 2010 3:18

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  14. Content type: Research

    Simultaneous saccharification and co-fermentation (SSCF) has been recognized as a feasible option for ethanol production from xylose-rich lignocellulosic materials. To reach high ethanol concentration in the b...

    Authors: Kim Olofsson, Benny Palmqvist and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2010 3:17

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  15. Content type: Research

    Bioethanol can be produced from sugar-rich, starch-rich (first generation; 1G) or lignocellulosic (second generation; 2G) raw materials. Integration of 2G ethanol with 1G could facilitate the introduction of t...

    Authors: Borbála Erdei, Zsolt Barta, Bálint Sipos, Kati Réczey, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2010 3:16

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  16. Content type: Research

    The two-step dilute acid hydrolysis (DAH) of softwood is costly in energy demands and capital costs. However, it has the advantage that hydrolysis and subsequent removal of hemicellulose-derived sugars can be ...

    Authors: Peter Bösch, Ola Wallberg, Elisabeth Joelsson, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2010 3:15

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  17. Content type: Research

    To make lignocellulosic fuel ethanol economically competitive with fossil fuels, it is necessary to reduce the production cost. One way to achieve this is by increasing the substrate concentration in the produ...

    Authors: Kerstin Hoyer, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2010 3:14

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  18. Content type: Research

    Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by Saccharomyces cerevisiae requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to...

    Authors: Rosa Garcia Sanchez, Kaisa Karhumaa, César Fonseca, Violeta Sànchez Nogué, João RM Almeida, Christer U Larsson, Oskar Bengtsson, Maurizio Bettiga, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2010 3:13

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  19. Content type: Research

    Corn grain is an important renewable source for bioethanol production in the USA. Corn ethanol is currently produced by steam liquefaction of starch-rich grains followed by enzymatic saccharification and ferme...

    Authors: Qianjun Shao, Shishir PS Chundawat, Chandraraj Krishnan, Bryan Bals, Leonardo da Costa Sousa, Kurt D Thelen, Bruce E Dale and Venkatesh Balan

    Citation: Biotechnology for Biofuels 2010 3:12

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  20. Content type: Research

    Fermentations using Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST), and Zymomonas mobilis AX101 are compared side-by-side on corn steep liquor (CSL) media and the water extract and enzymatic hydroly...

    Authors: Ming W Lau, Christa Gunawan, Venkatesh Balan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2010 3:11

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  21. Content type: Research

    Although measurements of crystallinity index (CI) have a long history, it has been found that CI varies significantly depending on the choice of measurement method. In this study, four different techniques inc...

    Authors: Sunkyu Park, John O Baker, Michael E Himmel, Philip A Parilla and David K Johnson

    Citation: Biotechnology for Biofuels 2010 3:10

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  22. Content type: Research

    Grasses are relatively recalcitrant to genetic transformation in comparison to certain dicotyledons, yet they constitute some of the most important biofuel crops. Genetic transformation of switchgrass (Panicum vi...

    Authors: Xinlu Chen, Raymie Equi, Holly Baxter, Kyle Berk, Jin Han, Sujata Agarwal and Janice Zale

    Citation: Biotechnology for Biofuels 2010 3:9

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  23. Content type: Research

    US legislation requires the use of advanced biofuels to be made from non-food feedstocks. However, commercialization of lignocellulosic ethanol technology is more complex than expected and is therefore running...

    Authors: NP Nghiem, KB Hicks, DB Johnston, G Senske, M Kurantz, M Li, J Shetty and G Konieczny-Janda

    Citation: Biotechnology for Biofuels 2010 3:8

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  24. Content type: Research

    The conditions for steam pretreatment of sugar cane bagasse and leaves were studied using CO2 as an impregnating agent. The following conditions were investigated: time (5 to 15 min) and temperature (190 to 220°C...

    Authors: Viridiana Ferreira-Leitão, Clarissa Cruz Perrone, Joice Rodrigues, Ana Paula Machado Franke, Stefano Macrelli and Guido Zacchi

    Citation: Biotechnology for Biofuels 2010 3:7

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  25. Content type: Research

    Different mechanistic models have been used in the literature to describe the enzymatic hydrolysis of pretreated biomass. Although these different models have been applied to different substrates, most of thes...

    Authors: Russell F Brown, Frank K Agbogbo and Mark T Holtzapple

    Citation: Biotechnology for Biofuels 2010 3:6

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  26. Content type: Research

    The enzymatic hydrolysis of cellulose is still considered as one of the main limiting steps of the biological production of biofuels from lignocellulosic biomass. It is a complex multistep process, and various...

    Authors: Marie Chauve, Hugues Mathis, Delphine Huc, Dominique Casanave, Frédéric Monot and Nicolas Lopes Ferreira

    Citation: Biotechnology for Biofuels 2010 3:3

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  27. Content type: Research

    Biofuels offer a viable alternative to petroleum-based fuel. However, current methods are not sufficient and the technology required in order to use lignocellulosic biomass as a fermentation substrate faces se...

    Authors: Sandra A Allen, William Clark, J Michael McCaffery, Zhen Cai, Alison Lanctot, Patricia J Slininger, Z Lewis Liu and Steven W Gorsich

    Citation: Biotechnology for Biofuels 2010 3:2

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  28. Content type: Research

    When producing biofuels from dedicated feedstock, agronomic factors such as harvest time and location can impact the downstream production. Thus, this paper studies the effectiveness of ammonia fibre expansion...

    Authors: Bryan Bals, Chad Rogers, Mingjie Jin, Venkatesh Balan and Bruce Dale

    Citation: Biotechnology for Biofuels 2010 3:1

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  29. Content type: Research

    In this study, the dilute maleic acid pretreatment of wheat straw is optimized, using pretreatment time, temperature and maleic acid concentration as design variables. A central composite design was applied to...

    Authors: A Maarten J Kootstra, Hendrik H Beeftink, Elinor L Scott and Johan PM Sanders

    Citation: Biotechnology for Biofuels 2009 2:31

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  30. Content type: Research

    Pretreatment chemistry is of central importance due to its impacts on cellulosic biomass processing and biofuels conversion. Ammonia fiber expansion (AFEX) and dilute acid are two promising pretreatments using...

    Authors: Ming W Lau, Christa Gunawan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2009 2:30

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  31. Content type: Research

    Corn stover composition changes considerably throughout the growing season and also varies between the various fractions of the plant. These differences can impact optimal pretreatment conditions, enzymatic di...

    Authors: Rebecca J Garlock, Shishir PS Chundawat, Venkatesh Balan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2009 2:29

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  32. Content type: Research

    Screening new lignocellulosic biomass pretreatments and advanced enzyme systems at process relevant conditions is a key factor in the development of economically viable lignocellulosic ethanol. Shake flasks, t...

    Authors: Christine M Roche, Clare J Dibble and Jonathan J Stickel

    Citation: Biotechnology for Biofuels 2009 2:28

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  33. Content type: Research

    Termite lignocellulose digestion is achieved through a collaboration of host plus prokaryotic and eukaryotic symbionts. In the present work, we took a combined host and symbiont metatranscriptomic approach for...

    Authors: Aurélien Tartar, Marsha M Wheeler, Xuguo Zhou, Monique R Coy, Drion G Boucias and Michael E Scharf

    Citation: Biotechnology for Biofuels 2009 2:25

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  34. Content type: Research

    Bioethanol isolated from lignocellulosic biomass represents one of the most promising renewable and carbon neutral alternative liquid fuel sources. Enzymatic saccharification using cellulase has proven to be a...

    Authors: Tatsuya Fujii, Xu Fang, Hiroyuki Inoue, Katsuji Murakami and Shigeki Sawayama

    Citation: Biotechnology for Biofuels 2009 2:24

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  35. Content type: Commentary

    Mary E H Mandels, who spearheaded the US Army's national bioconversion studies for four decades and was an early proponent of conversion of waste biomass to readily bioconvertible sugars for the production of ...

    Authors: Fred Allen, Raymond Andreotti, Douglas E Eveleigh and John Nystrom

    Citation: Biotechnology for Biofuels 2009 2:22

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  36. Content type: Commentary

    This article sets forth a simple cellulase assay procedure. Cellulose is variable in nature, insoluble and resistant to enzymatic attack. As a result there have been a bevy of bewildering cellulase assays publ...

    Authors: Douglas E Eveleigh, Mary Mandels, Raymond Andreotti and Charles Roche

    Citation: Biotechnology for Biofuels 2009 2:21

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  37. Content type: Commentary

    This article, 'Reflections on the United States Military 1941-1987' written by my grandmother, Mary Mandels, illustrates her passion for life. Her outreach article was considered most appropriate for publicati...

    Authors: Mary Mandels and Douglas E Eveleigh

    Citation: Biotechnology for Biofuels 2009 2:20

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  38. Content type: Review

    Hypocrea jecorina (= Trichoderma reesei) is the main industrial source of cellulases and hemicellulases used to depolymerise plant biomass to simple sugars that are converted to chemical intermediates and biofuel...

    Authors: Christian P Kubicek, Marianna Mikus, André Schuster, Monika Schmoll and Bernhard Seiboth

    Citation: Biotechnology for Biofuels 2009 2:19

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  39. Content type: Research

    Two economic factors make watermelon worthy of consideration as a feedstock for ethanol biofuel production. First, about 20% of each annual watermelon crop is left in the field because of surface blemishes or ...

    Authors: Wayne W Fish, Benny D Bruton and Vincent M Russo

    Citation: Biotechnology for Biofuels 2009 2:18

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  40. Content type: Research

    Drying is currently the most frequently used conservation method for cereal grain, which in temperate climates consumes a major part of process energy. Airtight storage of moist feed grain using the biocontrol...

    Authors: Volkmar Passoth, Anna Eriksson, Mats Sandgren, Jerry Ståhlberg, Kathleen Piens and Johan Schnürer

    Citation: Biotechnology for Biofuels 2009 2:16

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  41. Content type: Research

    Improvement of the process of cellulase production and development of more efficient lignocellulose-degrading enzymes are necessary in order to reduce the cost of enzymes required in the biomass-to-bioethanol ...

    Authors: Krisztina Kovacs, Stefano Macrelli, George Szakacs and Guido Zacchi

    Citation: Biotechnology for Biofuels 2009 2:14

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