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  1. Sugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high ...

    Authors: Guoquan Liu, Yan Zhang, Hao Gong, Shan Li, Yunrong Pan, Christopher Davis, Hai-Chun Jing, Luguang Wu and Ian D. Godwin
    Citation: Biotechnology for Biofuels 2021 14:53
  2. The amount of carbon dioxide in the atmosphere has been on the rise for more than a century. Bioenergy crops are seen by the Intergovernmental Panel on Climate Change as an essential part of the solution to ad...

    Authors: Obste Therasme, Timothy A. Volk, Mark H. Eisenbies, Thomas E. Amidon and Marie-Odile Fortier
    Citation: Biotechnology for Biofuels 2021 14:52
  3. Lytic polysaccharide monooxygenases (LPMOs) are important industrial enzymes known for their catalytic degradation of recalcitrant polymers such as cellulose or chitin. Their activity can be measured by length...

    Authors: Søren Brander, Stine Lausten, Johan Ø. Ipsen, Kristoffer B. Falkenberg, Andreas B. Bertelsen, Morten H. H. Nørholm, Lars H. Østergaard and Katja S. Johansen
    Citation: Biotechnology for Biofuels 2021 14:51
  4. Hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT) is a central enzyme of the so-called “esters” pathway to monolignols. As originally envisioned, HCT functions twice in this pathway, to form c...

    Authors: Juan Carlos Serrani-Yarce, Luis Escamilla-Trevino, Jaime Barros, Lina Gallego-Giraldo, Yunqiao Pu, Art Ragauskas and Richard A. Dixon
    Citation: Biotechnology for Biofuels 2021 14:50
  5. Ionic liquids (ILs) are promising pretreatment solvents for lignocellulosic biomass, but are largely prepared from petroleum precursors. Benzaldehydes from depolymerized lignin, such as vanillin, syringaldehyd...

    Authors: Shihong Liu, Michael Gonzalez, Celine Kong, Scott Weir and Aaron M. Socha
    Citation: Biotechnology for Biofuels 2021 14:47
  6. Biochemical conversion of lignocellulosic biomass to simple sugars at commercial scale is hampered by the high cost of saccharifying enzymes. Lytic polysaccharide monooxygenases (LPMOs) may hold the key to ove...

    Authors: Adnan Kadić, Anikó Várnai, Vincent G. H. Eijsink, Svein Jarle Horn and Gunnar Lidén
    Citation: Biotechnology for Biofuels 2021 14:46
  7. It is interesting to modify sugar metabolic pathways to improve the productivity of biocatalysts that convert sugars to value-added products. However, this attempt often fails due to the tight control of the s...

    Authors: Toru Jojima, Takafumi Igari, Ryoji Noburyu, Akira Watanabe, Masako Suda and Masayuki Inui
    Citation: Biotechnology for Biofuels 2021 14:45
  8. Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance of woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification of the chemical composition ...

    Authors: Madhavi Latha Gandla, Niklas Mähler, Sacha Escamez, Tomas Skotare, Ogonna Obudulu, Linus Möller, Ilka N. Abreu, Joakim Bygdell, Magnus Hertzberg, Torgeir R. Hvidsten, Thomas Moritz, Gunnar Wingsle, Johan Trygg, Hannele Tuominen and Leif J. Jönsson
    Citation: Biotechnology for Biofuels 2021 14:43
  9. The global demand for functional proteins is extensive, diverse, and constantly increasing. Medicine, agriculture, and industrial manufacturing all rely on high-quality proteins as major active components or p...

    Authors: Jennifer A. Schmidt, Lubna V. Richter, Lisa A. Condoluci and Beth A. Ahner
    Citation: Biotechnology for Biofuels 2021 14:42
  10. Baker’s yeast is a widely used eukaryotic cell factory, producing a diverse range of compounds including biofuels and fine chemicals. The use of lignocellulose as feedstock offers the opportunity to run these ...

    Authors: Friederike Gutmann, Cosimo Jann, Filipa Pereira, Andreas Johansson, Lars M. Steinmetz and Kiran R. Patil
    Citation: Biotechnology for Biofuels 2021 14:41
  11. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Jeffrey P. Tingley, Kristin E. Low, Xiaohui Xing and D. Wade Abbott
    Citation: Biotechnology for Biofuels 2021 14:40

    The original article was published in Biotechnology for Biofuels 2021 14:16

  12. Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial ferment...

    Authors: Nozomu Shibata, Hiroshi Kakeshita, Kazuaki Igarashi, Yasushi Takimura, Yosuke Shida, Wataru Ogasawara, Tohru Koda, Tomohisa Hasunuma and Akihiko Kondo
    Citation: Biotechnology for Biofuels 2021 14:39
  13. Saccharomyces cerevisiae is well-known as an ideal model system for basic research and important industrial microorganism for biotechnological applications. Acetic acid is an important growth inhibitor that has d...

    Authors: Yanfei Cheng, Hui Zhu, Zhengda Du, Xuena Guo, Chenyao Zhou, Zhaoyue Wang and Xiuping He
    Citation: Biotechnology for Biofuels 2021 14:38
  14. Woody plants with high glucose content are alternative bioresources for the production of biofuels and biochemicals. Various pretreatment methods may be used to reduce the effects of retardation factors such a...

    Authors: Dae-Seok Lee, Yoon-Gyo Lee, Eun Jin Cho, Younho Song and Hyeun-Jong Bae
    Citation: Biotechnology for Biofuels 2021 14:37
  15. Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis;...

    Authors: Lin Dai, Tian Huang, Kankan Jiang, Xin Zhou and Yong Xu
    Citation: Biotechnology for Biofuels 2021 14:35
  16. Butanol (n-butanol) has been gaining attention as a renewable energy carrier and an alternative biofuel with superior properties to the most widely used ethanol. We performed 48 anaerobic fermentations simultaneo...

    Authors: Cansu Birgen, Kristin F. Degnes, Sidsel Markussen, Alexander Wentzel and Håvard Sletta
    Citation: Biotechnology for Biofuels 2021 14:34
  17. Low- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental glucose fluctuation for their growth and competitiveness. In Neurospora crassa, the dual-affinit...

    Authors: Jinyang Li, Qian Liu, Jingen Li, Liangcai Lin, Xiaolin Li, Yongli Zhang and Chaoguang Tian
    Citation: Biotechnology for Biofuels 2021 14:33
  18. One of the sustainable development goals focuses on the biomass-based production as a replacement for fossil-based commodities. A novel feedstock with vast potentials is tunicate biomass, which can be pretreat...

    Authors: Kateřina Hrůzová, Leonidas Matsakas, Anthi Karnaouri, Fredrik Norén, Ulrika Rova and Paul Christakopoulos
    Citation: Biotechnology for Biofuels 2021 14:32
  19. Penicillium funiculosum NCIM1228 is a non-model filamentous fungus that produces high-quality secretome for lignocellulosic biomass saccharification. Despite having desirable traits to be an industrial workhorse,...

    Authors: Anmoldeep Randhawa, Nandita Pasari, Tulika Sinha, Mayank Gupta, Anju M. Nair, Olusola A. Ogunyewo, Sandhya Verma, Praveen Kumar Verma and Syed Shams Yazdani
    Citation: Biotechnology for Biofuels 2021 14:31
  20. The discovery of lytic polysaccharide monooxygenases (LPMOs) has fundamentally changed our understanding of microbial lignocellulose degradation. Cellulomonas bacteria have a rich history of study due to their ab...

    Authors: James Li, Laleh Solhi, Ethan D. Goddard-Borger, Yann Mathieu, Warren W. Wakarchuk, Stephen G. Withers and Harry Brumer
    Citation: Biotechnology for Biofuels 2021 14:29
  21. Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative depolymerization of industrially relevant crystalline polysaccharides, such as cellulose, in a reaction that depends o...

    Authors: Anton A. Stepnov, Zarah Forsberg, Morten Sørlie, Giang-Son Nguyen, Alexander Wentzel, Åsmund K. Røhr and Vincent G. H. Eijsink
    Citation: Biotechnology for Biofuels 2021 14:28
  22. Lignin intermediates resulting from lignocellulose degradation have been suspected to hinder anaerobic mineralisation of organic materials to biogas. Phenyl acids like phenylacetate (PAA) are early detectable ...

    Authors: Eva Maria Prem, Mira Mutschlechner, Blaz Stres, Paul Illmer and Andreas Otto Wagner
    Citation: Biotechnology for Biofuels 2021 14:27
  23. Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasin...

    Authors: Hsiang-Yen Su, Hua-Ying Li, Cai-Yun Xie, Qiang Fei and Ke-Ke Cheng
    Citation: Biotechnology for Biofuels 2021 14:26
  24. Humic acids (HA) have been used in biorefinery process due to its surfactant properties as an aid to the pretreatment of lignocellulose, with results indicating a positive effect on delignification. However, t...

    Authors: Wei Tang, Xinxing Wu, Caoxing Huang, Zhe Ling, Chenhuan Lai and Qiang Yong
    Citation: Biotechnology for Biofuels 2021 14:25
  25. The cellulolytic thermophile Clostridium thermocellum is an important biocatalyst due to its ability to solubilize lignocellulosic feedstocks without the need for pretreatment or exogenous enzyme addition. At low...

    Authors: Dhananjay Beri, Christopher D. Herring, Sofie Blahova, Suresh Poudel, Richard J. Giannone, Robert L. Hettich and Lee R. Lynd
    Citation: Biotechnology for Biofuels 2021 14:24
  26. The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succin...

    Authors: Tianwen Zheng, Bin Xu, Yaliang Ji, Wenming Zhang, Fengxue Xin, Weiliang Dong, Ping Wei, Jiangfeng Ma and Min Jiang
    Citation: Biotechnology for Biofuels 2021 14:23
  27. Both APETALA2/Ethylene Responsive Factor (AP2/ERF) superfamily and R2R3-MYB family were from one of the largest diverse families of transcription factors (TFs) in plants, and played important roles in plant de...

    Authors: Guanlan Xing, Jinyu Li, Wenli Li, Sin Man Lam, Hongli Yuan, Guanghou Shui and Jinshui Yang
    Citation: Biotechnology for Biofuels 2021 14:22
  28. Predatory flagellates and ciliates are two common bio-contaminants which frequently cause biomass losses in Chlorella mass culture. Efficient and targeted ways are required to control these contaminations in Chlo...

    Authors: Xiaobin Wen, Aoqi Zhang, Xiaoyan Zhu, Lin Liang, Yan Huo, Kaixuan Wang, Youzhi Yu, Yahong Geng, Yi Ding and Yeguang Li
    Citation: Biotechnology for Biofuels 2021 14:21
  29. Microalgae have attracted considerable interest due to their ability to produce a wide range of valuable compounds. Pulsed Electric Fields (PEF) has been demonstrated to effectively disrupt the microalgae cell...

    Authors: I. Papachristou, S. Akaberi, A. Silve, E. Navarro-López, R. Wüstner, K. Leber, N. Nazarova, G. Müller and W. Frey
    Citation: Biotechnology for Biofuels 2021 14:20
  30. Increasing the efficiency of the biogas production process is possible by modifying the technological installations of the biogas plant. In this study, specific solutions based on a mathematical model that lea...

    Authors: Karol Postawa, Jerzy Szczygieł and Marek Kułażyński
    Citation: Biotechnology for Biofuels 2021 14:19
  31. Biomass composition varies from plant to plant and greatly affects biomass utilization. Lignin is a heterogeneous phenolic polymer derived mainly from p-coumaryl, coniferyl, and sinapyl alcohols and makes up to 1...

    Authors: Fang Chen, Chunliu Zhuo, Xirong Xiao, Thomas H. Pendergast and Katrien M. Devos
    Citation: Biotechnology for Biofuels 2021 14:18
  32. The production of biofuels as an efficient source of renewable energy has received considerable attention due to increasing energy demands and regulatory incentives to reduce greenhouse gas emissions. Second-g...

    Authors: Jeffrey P. Tingley, Kristin E. Low, Xiaohui Xing and D. Wade Abbott
    Citation: Biotechnology for Biofuels 2021 14:16

    The Correction to this article has been published in Biotechnology for Biofuels 2021 14:40

  33. Bacillus subtilis CS13 was previously isolated for 2,3-butanediol (2,3-BD) and poly-γ-glutamic acid (γ-PGA) co-production. When culturing this strain without L-glutamic acid in the medium, 2,3-BD is the main meta...

    Authors: Dexin Wang, Baek-Rock Oh, Sungbeom Lee, Dae-Hyuk Kim and Min-Ho Joe
    Citation: Biotechnology for Biofuels 2021 14:15
  34. Multidimensional solid-state nuclear magnetic resonance (ssNMR) spectroscopy has emerged as an indispensable technique for resolving polymer structure and intermolecular packing in primary and secondary plant ...

    Authors: Wancheng Zhao, Alex Kirui, Fabien Deligey, Frederic Mentink-Vigier, Yihua Zhou, Baocai Zhang and Tuo Wang
    Citation: Biotechnology for Biofuels 2021 14:14
  35. Plastic plays a crucial role in everyday life of human living, nevertheless it represents an undeniable source of land and water pollution. Polyhydroxybutyrate (PHB) is a bio-based and biodegradable polyester,...

    Authors: Beatrice Mongili, Annalisa Abdel Azim, Silvia Fraterrigo Garofalo, Esperanza Batuecas, Angela Re, Sergio Bocchini and Debora Fino
    Citation: Biotechnology for Biofuels 2021 14:13
  36. Despite the environmental value of biobased lubricants, they account for less than 2% of global lubricant use due to poor thermo-oxidative stability arising from the presence of unsaturated double bonds. Methy...

    Authors: Hannah G. Blitzblau, Andrew L. Consiglio, Paulo Teixeira, Donald V. Crabtree, Shuyan Chen, Oliver Konzock, Gamuchirai Chifamba, Austin Su, Annapurna Kamineni, Kyle MacEwen, Maureen Hamilton, Vasiliki Tsakraklides, Jens Nielsen, Verena Siewers and A. Joe Shaw
    Citation: Biotechnology for Biofuels 2021 14:12
  37. Efficient utilization of all available carbons from lignocellulosic biomass is critical for economic efficiency of a bioconversion process to produce renewable bioproducts. However, the metabolic responses tha...

    Authors: Zhangyang Xu, Chunmei Pan, Xiaolu Li, Naijia Hao, Tong Zhang, Matthew J. Gaffrey, Yunqiao Pu, John R. Cort, Arthur J. Ragauskas, Wei-Jun Qian and Bin Yang
    Citation: Biotechnology for Biofuels 2021 14:11
  38. Bioethanol as a renewable energy resource plays an important role in alleviating energy crisis and environmental protection. Pervaporation has achieved increasing attention because of its potential to be a use...

    Authors: Ping Peng, Yongqiang Lan, Lun Liang and Kemeng Jia
    Citation: Biotechnology for Biofuels 2021 14:10
  39. Ethanol biorefineries need to lower their overall production costs to become economically feasible. Two strategies to achieve this are to reduce costs using cheaper feedstocks or to increase the ethanol produc...

    Authors: Danielle Uchimura Pascoli, Azra Suko, Rick Gustafson, Heidi L. Gough and Renata Bura
    Citation: Biotechnology for Biofuels 2021 14:9
  40. Isobutanol is a candidate to replace gasoline from fossil resources. This higher alcohol can be produced from sugars using genetically modified microorganisms. Shimwellia blattae (p424IbPSO) is a robust strain re...

    Authors: Miguel G. Acedos, Isabel de la Torre, Victoria E. Santos, Félix García-Ochoa, José L. García and Beatriz Galán
    Citation: Biotechnology for Biofuels 2021 14:8
  41. It is desirable to improve the anaerobic digestion processes of recalcitrant materials, such as cellulose. Enhancement of methane (CH4) production from organic molecules was previously accomplished through coupli...

    Authors: Kengo Sasaki, Daisuke Sasaki, Yota Tsuge, Masahiko Morita and Akihiko Kondo
    Citation: Biotechnology for Biofuels 2021 14:7
  42. The issues of global warming, coupled with fossil fuel depletion, have undoubtedly led to renewed interest in other sources of commercial fuels. The search for renewable fuels has motivated research into the b...

    Authors: Mobolaji Felicia Adegboye, Omena Bernard Ojuederie, Paola M. Talia and Olubukola Oluranti Babalola
    Citation: Biotechnology for Biofuels 2021 14:5
  43. 2-phenylethanol (2-PE) is a rose-scented flavor and fragrance compound that is used in food, beverages, and personal care products. Compatibility with gasoline also makes it a potential biofuel or fuel additiv...

    Authors: Mengwan Li, Xuye Lang, Marcos Moran Cabrera, Sawyer De Keyser, Xiyan Sun, Nancy Da Silva and Ian Wheeldon
    Citation: Biotechnology for Biofuels 2021 14:3
  44. Cutaneotrichosporon oleaginosus ATCC 20509 is a fast-growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel produ...

    Authors: Nhung Pham, Maarten Reijnders, Maria Suarez-Diez, Bart Nijsse, Jan Springer, Gerrit Eggink and Peter J. Schaap
    Citation: Biotechnology for Biofuels 2021 14:2