Articles
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Citation: Biotechnology for Biofuels 2016 9:263
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Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate production
Succinate biosynthesis of Escherichia coli is reducing equivalent-dependent and the EMP pathway serves as the primary reducing equivalent source under anaerobic condition. Compared with EMP, pentose phosphate pat...
Citation: Biotechnology for Biofuels 2016 9:262 -
Biorefinery cascade processing for creating added value on tomato industrial by-products from Tunisia
In today’s consumer perception of industrial processes and food production, aspects like food quality, human health, environmental safety, and energy security have become the keywords. Therefore, much effort h...
Citation: Biotechnology for Biofuels 2016 9:261 -
A polysaccharide utilization locus from Flavobacterium johnsoniae enables conversion of recalcitrant chitin
Chitin is the second most abundant polysaccharide on earth and as such a great target for bioconversion applications. The phylum Bacteroidetes is one of nature’s most ubiquitous bacterial lineages and is essen...
Citation: Biotechnology for Biofuels 2016 9:260 -
Study of Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola for their lipogenic aptitude from different carbon sources
The ability of some microorganisms to accumulate lipids is well known; however, only recently the number of studies on microbial lipid biosynthesis for obtaining oleochemical products, namely biofuels and some...
Citation: Biotechnology for Biofuels 2016 9:259 -
Genome and methylome of the oleaginous diatom Cyclotella cryptica reveal genetic flexibility toward a high lipid phenotype
Improvement in the performance of eukaryotic microalgae for biofuel and bioproduct production is largely dependent on characterization of metabolic mechanisms within the cell. The marine diatom Cyclotella cryptic...
Citation: Biotechnology for Biofuels 2016 9:258 -
Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression
n-Butanol can serve as an excellent gasoline substitute. Naturally, it is produced by some Clostridia species which, however, exhibit only limited suitability for industrial n-butanol production. The yeast Saccha...
Citation: Biotechnology for Biofuels 2016 9:257 -
In situ label-free imaging of hemicellulose in plant cell walls using stimulated Raman scattering microscopy
Plant hemicellulose (largely xylan) is an excellent feedstock for renewable energy production and second only to cellulose in abundance. Beyond a source of fermentable sugars, xylan constitutes a critical poly...
Citation: Biotechnology for Biofuels 2016 9:256 -
The dilemma for lipid productivity in green microalgae: importance of substrate provision in improving oil yield without sacrificing growth
Rising oil prices and concerns over climate change have resulted in more emphasis on research into renewable biofuels from microalgae. Unlike plants, green microalgae have higher biomass productivity, will not...
Citation: Biotechnology for Biofuels 2016 9:255 -
Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors
Biohythane production via two-stage fermentation is a promising direction for sustainable energy recovery from lignocellulosic biomass. However, the utilization of lignocellulosic biomass suffers from specific...
Citation: Biotechnology for Biofuels 2016 9:254 -
Fungal fermentation on anaerobic digestate for lipid-based biofuel production
Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large por...
Citation: Biotechnology for Biofuels 2016 9:253 -
Whole-genome metabolic model of Trichoderma reesei built by comparative reconstruction
Trichoderma reesei is one of the main sources of biomass-hydrolyzing enzymes for the biotechnology industry. There is a need for improving its enzyme production efficiency. The use of ...
Citation: Biotechnology for Biofuels 2016 9:252 -
Improved sugar yields from biomass sorghum feedstocks: comparing low-lignin mutants and pretreatment chemistries
For biofuel production processes to be economically efficient, it is essential to maximize the production of monomeric carbohydrates from the structural carbohydrates of feedstocks. One strategy for maximizing...
Citation: Biotechnology for Biofuels 2016 9:251 -
Furfural tolerance and detoxification mechanism in Candida tropicalis
Current biomass pretreatment by hydrothermal treatment (including acid hydrolysis, steam explosion, and high-temperature steaming) and ionic liquids generally generate inhibitors to the following fermentation ...
Citation: Biotechnology for Biofuels 2016 9:250 -
Redox dependent metabolic shift in Clostridium autoethanogenum by extracellular electron supply
Microbial electrosynthesis is a novel approach that aims at shifting the cellular metabolism towards electron-dense target products by extracellular electron supply. Many organisms including several acetogenic...
Citation: Biotechnology for Biofuels 2016 9:249 -
Production of l(+)-lactic acid from acid pretreated sugarcane bagasse using Bacillus coagulans DSM2314 in a simultaneous saccharification and fermentation strategy
Sugars derived from lignocellulose-rich sugarcane bagasse can be used as feedstock for production of l(+)-lactic acid, a precursor for renewable bioplastics. In our research, acid-pretreated bagasse was hydrolyse...
Citation: Biotechnology for Biofuels 2016 9:248 -
Improvement of catalytic performance of lignin peroxidase for the enhanced degradation of lignocellulose biomass based on the imbedded electron-relay in long-range electron transfer route
Although lignin peroxidase is claimed as a key enzyme in enzyme-catalyzed lignin degradation, in vitro enzymatic degradation of lignin was not easily observed in lab-scale experiments. It implies that other fa...
Citation: Biotechnology for Biofuels 2016 9:247 -
Whole-genome de novo sequencing, combined with RNA-Seq analysis, reveals unique genome and physiological features of the amylolytic yeast Saccharomycopsis fibuligera and its interspecies hybrid
Genomic studies on fungal species with hydrolytic activity have gained increased attention due to their great biotechnological potential for biomass-based biofuel production. The amylolytic yeast Saccharomycopsis...
Citation: Biotechnology for Biofuels 2016 9:246 -
Flowthrough pretreatment with very dilute acid provides insights into high lignin contribution to biomass recalcitrance
Flowthrough pretreatment is capable of removing much higher quantities of hemicellulose and lignin from lignocellulosic biomass than batch pretreatment performed at otherwise similar conditions. Comparison of ...
Citation: Biotechnology for Biofuels 2016 9:245 -
Presence does not imply activity: DNA and RNA patterns differ in response to salt perturbation in anaerobic digestion
The microbial community in anaerobic digestion is mainly monitored by means of DNA-based methods. This may lead to incorrect interpretation of the community parameters, because microbial abundance does not nec...
Citation: Biotechnology for Biofuels 2016 9:244 -
Metagenomic and metaproteomic analyses of a corn stover-adapted microbial consortium EMSD5 reveal its taxonomic and enzymatic basis for degrading lignocellulose
Microbial consortia represent promising candidates for aiding in the development of plant biomass conversion strategies for biofuel production. However, the interaction between different community members and ...
Citation: Biotechnology for Biofuels 2016 9:243 -
Comparison of ethanol production from corn cobs and switchgrass following a pyrolysis-based biorefinery approach
One of the main obstacles in lignocellulosic ethanol production is the necessity of pretreatment and fractionation of the biomass feedstocks to produce sufficiently pure fermentable carbohydrates. In addition,...
Citation: Biotechnology for Biofuels 2016 9:242 -
What could the entire cornstover contribute to the enhancement of waste activated sludge acidification? Performance assessment and microbial community analysis
Volatile fatty acids (VFAs) production from waste activated sludge (WAS) digestion is constrained by unbalanced nutrient composition (low carbon-to-nitrogen ratio). Characteristics conditioning by extra carbon...
Citation: Biotechnology for Biofuels 2016 9:241 -
Long-term adaptive evolution of Leuconostoc mesenteroides for enhancement of lactic acid tolerance and production
Lactic acid has been approved by the United States Food and Drug Administration as Generally Regarded As Safe (GRAS) and is commonly used in the cosmetics, pharmaceutical, and food industries. Applications of ...
Citation: Biotechnology for Biofuels 2016 9:240 -
Bioinformatic characterization of type-specific sequence and structural features in auxiliary activity family 9 proteins
Due to the impending depletion of fossil fuels, it has become important to identify alternative energy sources. The biofuel industry has proven to be a promising alternative. However, owing to the complex natu...
Citation: Biotechnology for Biofuels 2016 9:239 -
Valorization of lignin and cellulose in acid-steam-exploded corn stover by a moderate alkaline ethanol post-treatment based on an integrated biorefinery concept
Due to the unsustainable consumption of fossil resources, great efforts have been made to convert lignocellulose into bioethanol and commodity organic compounds through biological methods. The conversion of ce...
Citation: Biotechnology for Biofuels 2016 9:238 -
Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate
Interannual variability in precipitation, particularly drought, can affect lignocellulosic crop biomass yields and composition, and is expected to increase biofuel yield variability. However, the effect of pre...
Citation: Biotechnology for Biofuels 2016 9:237 -
Piezo-tolerant natural gas-producing microbes under accumulating pCO2
It is known that a part of natural gas is produced by biogenic degradation of organic matter, but the microbial pathways resulting in the formation of pressurized gas fields remain unknown. Autogeneration of b...
Citation: Biotechnology for Biofuels 2016 9:236 -
Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer
Diatoms have attracted interest as biofuel producers. Here, the contents of lipids and photosynthetic pigments were analyzed in a marine centric diatom, Chaetoceros gracilis. This diatom can be genetically engine...
Citation: Biotechnology for Biofuels 2016 9:235 -
Comparison of aldehyde-producing activities of cyanobacterial acyl-(acyl carrier protein) reductases
Biosynthesis of alkanes is an attractive way of producing substitutes for petroleum-based alkanes. Acyl-[acyl carrier protein (ACP)] reductase (AAR) is a key enzyme for alkane biosynthesis in cyanobacteria and...
Citation: Biotechnology for Biofuels 2016 9:234 -
Editorial: chemicals and bioproducts from biomass
Citation: Biotechnology for Biofuels 2016 9:233 -
Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae
Saccharomyces cerevisiae has already been used for heterologous production of fuel chemicals and valuable natural products. The establishment of complicated heterologous biosynthetic p...
Citation: Biotechnology for Biofuels 2016 9:232 -
Diversity of fungal feruloyl esterases: updated phylogenetic classification, properties, and industrial applications
Feruloyl esterases (FAEs) represent a diverse group of carboxyl esterases that specifically catalyze the hydrolysis of ester bonds between ferulic (hydroxycinnamic) acid and plant cell wall polysaccharides. Th...
Citation: Biotechnology for Biofuels 2016 9:231 -
Effective isopropanol–butanol (IB) fermentation with high butanol content using a newly isolated Clostridium sp. A1424
Acetone–butanol–ethanol fermentation has been studied for butanol production. Alternatively, to achieve acetone-free butanol production, use of clostridium strains producing butanol and 1,3-propanediol (1,3-PD...
Citation: Biotechnology for Biofuels 2016 9:230 -
A systems analysis of biodiesel production from wheat straw using oleaginous yeast: process design, mass and energy balances
Biodiesel is the main liquid biofuel in the EU and is currently mainly produced from vegetable oils. Alternative feedstocks are lignocellulosic materials, which provide several benefits compared with many exis...
Citation: Biotechnology for Biofuels 2016 9:229 -
Dry-grind processing using amylase corn and superior yeast to reduce the exogenous enzyme requirements in bioethanol production
Conventional corn dry-grind ethanol production process requires exogenous alpha and glucoamylases enzymes to breakdown starch into glucose, which is fermented to ethanol by yeast. This study evaluates the pote...
Citation: Biotechnology for Biofuels 2016 9:228 -
Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica
Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated pr...
Citation: Biotechnology for Biofuels 2016 9:227 -
Ectopic expression of AtDGAT1, encoding diacylglycerol O-acyltransferase exclusively committed to TAG biosynthesis, enhances oil accumulation in seeds and leaves of Jatropha
Jatropha curcas is an important biofuel crop due to the presence of high amount of oil in its seeds suitable for biodiesel production. Triacylglycerols (TAGs) are the most abundant for...
Citation: Biotechnology for Biofuels 2016 9:226 -
Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion
Plant lignocellulosic biomass is an abundant, renewable feedstock for the production of biobased fuels and chemicals. Previously, we showed that iron can act as a co-catalyst to improve the deconstruction of l...
Citation: Biotechnology for Biofuels 2016 9:225 -
Insight into the evolution of the proton concentration during autohydrolysis and dilute-acid hydrolysis of hemicellulose
During pretreatment, hemicellulose is removed from biomass via proton-catalyzed hydrolysis to produce soluble poly- and mono-saccharides. Many kinetic models have been proposed but the dependence of rate on pr...
Citation: Biotechnology for Biofuels 2016 9:224 -
Long-term variability in sugarcane bagasse feedstock compositional methods: sources and magnitude of analytical variability
In an effort to find economical, carbon-neutral transportation fuels, biomass feedstock compositional analysis methods are used to monitor, compare, and improve biofuel conversion processes. These methods are ...
Citation: Biotechnology for Biofuels 2016 9:223 -
Process design of SSCF for ethanol production from steam-pretreated, acetic-acid-impregnated wheat straw
Pretreatment is an important step in the production of ethanol from lignocellulosic material. Using acetic acid together with steam pretreatment allows the positive effects of an acid catalyst to be retained, ...
Citation: Biotechnology for Biofuels 2016 9:222 -
Aquatic plant Azolla as the universal feedstock for biofuel production
The quest for sustainable production of renewable and cheap biofuels has triggered an intensive search for domestication of the next generation of bioenergy crops. Aquatic plants which can rapidly colonize wet...
Citation: Biotechnology for Biofuels 2016 9:221 -
Comprehensive investigations of biobutanol production by a non-acetone and 1,3-propanediol generating Clostridium strain from glycerol and polysaccharides
Low-cost feedstocks, a single product (butanol), and a high butanol titer are three key points for establishing a sustainable and economically viable process for biological butanol production. Here, we compreh...
Citation: Biotechnology for Biofuels 2016 9:220 -
Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
Impactful greenhouse gas emissions abatement can now be achieved through gas fermentation using acetogenic microbes for the production of low-carbon fuels and chemicals. However, compared to traditional hosts ...
Citation: Biotechnology for Biofuels 2016 9:219 -
An enclosed rotating floating photobioreactor (RFP) powered by flowing water for mass cultivation of photosynthetic microalgae
The design of photobioreactor (PBR) for outdoor mass cultivation of microalgae determines the distribution of solar irradiance among cells in the culture, mode of agitation, mass transfer efficacy, and energy ...
Citation: Biotechnology for Biofuels 2016 9:218 -
Synergetic effect of dilute acid and alkali treatments on fractional application of rice straw
The biorefinery based on an effective and economical process is to fractionate the three primary constituents (cellulose, hemicelluloses, and lignin) from lignocellulosic biomass, in which the constituents can...
Citation: Biotechnology for Biofuels 2016 9:217 -
Efficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch-digesting glucoamylase from Penicillium oxalicum
Starch is a very abundant and renewable carbohydrate and is an important feedstock for industrial applications. The conventional starch liquefaction and saccharification processes are energy-intensive, complic...
Citation: Biotechnology for Biofuels 2016 9:216 -
High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties
Microbial synthesis of oleochemicals derived from native fatty acid (FA) metabolism has presented significant advances in recent years. Even so, native FA biosynthetic pathways often provide a narrow variety o...
Citation: Biotechnology for Biofuels 2016 9:215 -
Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction
Limonene, a monocyclic monoterpene, is known for its using as an important precursor of many flavoring, pharmaceutical, and biodiesel products. Currently, d-limonene has been produced via fractionation from es...
Citation: Biotechnology for Biofuels 2016 9:214