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  1. Laccases and laccase-like multicopper oxidases (LMCOs) oxidize a vast array of phenolic compounds and amines, releasing water as a byproduct. Their low substrate specificity is responsible for their tremendous...

    Authors: A. Zerva, C. Pentari, A. Termentzi, A. H. P. America, D. Zouraris, S. K. Bhattacharya, A. Karantonis, G. I. Zervakis and E. Topakas
    Citation: Biotechnology for Biofuels 2021 14:83
  2. The unicellular alga Haematococcus pluvialis has achieved considerable interests for its capacity to accumulate large amounts of triacylglycerol and astaxanthin under various environmental stresses. To our knowle...

    Authors: Qunju Hu, Danqiong Huang, Anguo Li, Zhangli Hu, Zhengquan Gao, Yongli Yang and Chaogang Wang
    Citation: Biotechnology for Biofuels 2021 14:82
  3. Most of the organic content of waste activated sludge (WAS) comprises microbial cells hard to degrade, which must be pre-treated for energy recovery by anaerobic digestion (AD). Electrooxidation pre-treatment ...

    Authors: J. A. Barrios, A. Cano, F. F. Rivera, M. E. Cisneros and U. Durán
    Citation: Biotechnology for Biofuels 2021 14:81
  4. Phytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme development has focused on increasing thermostability to withstand the hi...

    Authors: Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos H. Luna-Flores, Xiaojing Chen, Juhani von Hellens, Stephen Mahler and Robert Speight
    Citation: Biotechnology for Biofuels 2021 14:80
  5. Products made from recycled organic materials are an important part of a circular economy, but the question is whether they will be adopted by the public. Such products can elicit strong emotional responses an...

    Authors: Madeline Judge, Olivia de Hoog, Goda Perlaviciute, Nadja Contzen and Linda Steg
    Citation: Biotechnology for Biofuels 2021 14:79
  6. Plant cell wall-derived biomass serves as a renewable source of energy and materials with increasing importance. The cell walls are biomacromolecular assemblies defined by a fine arrangement of different class...

    Authors: Brian T DeVree, Lisa M Steiner, Sylwia Głazowska, Felix Ruhnow, Klaus Herburger, Staffan Persson and Jozef Mravec
    Citation: Biotechnology for Biofuels 2021 14:78
  7. Knowledge with respect to regulatory systems for cellulase production is prerequisite for exploitation of such regulatory networks to increase cellulase production, improve fermentation efficiency and reduce t...

    Authors: Ai-Ping Pang, Haiyan Wang, Funing Zhang, Xin Hu, Fu-Gen Wu, Zhihua Zhou, Wei Wang, Zuhong Lu and Fengming Lin
    Citation: Biotechnology for Biofuels 2021 14:77
  8. Engineering triacylglycerol (TAG) accumulation in vegetative tissues of non-food crops has become a promising way to meet our increasing demand for plant oils, especially the renewable production of biofuels. ...

    Authors: Yu Gao, Yan Sun, Huiling Gao, Ying Chen, Xiaoqing Wang, Jinai Xue, Xiaoyun Jia and Runzhi Li
    Citation: Biotechnology for Biofuels 2021 14:76

    The Correction to this article has been published in Biotechnology for Biofuels 2021 14:139

  9. Suberin is a hydrophobic biopolymer of significance in the production of biomass-derived materials and in biogeochemical cycling in terrestrial ecosystems. Here, we describe suberin structure and biosynthesis,...

    Authors: Anne E. Harman-Ware, Samuel Sparks, Bennett Addison and Udaya C. Kalluri
    Citation: Biotechnology for Biofuels 2021 14:75
  10. The transition to a biobased economy involving the depolymerization and fermentation of renewable agro-industrial sources is a challenge that can only be met by achieving the efficient hydrolysis of biomass to...

    Authors: Sophie C. Brandt, Hévila Brognaro, Arslan Ali, Bernhard Ellinger, Katharina Maibach, Martin Rühl, Carsten Wrenger, Hartmut Schlüter, Wilhelm Schäfer, Christian Betzel, Stefan Janssen and Martin Gand
    Citation: Biotechnology for Biofuels 2021 14:74
  11. Rhodotorula toruloides is a robust producer of triacylglycerol owing to its fast growth rate and strong metabolic flux under conditions of high cell density fermentation. However, the molecular basis of fatty aci...

    Authors: Yanbin Liu, Chong Mei John Koh, Sihui Amy Yap, Lin Cai and Lianghui Ji
    Citation: Biotechnology for Biofuels 2021 14:73
  12. In view of the natural resistance of hemicelluloses in lignocellulosic biomass on bioconversion of cellulose into fermentable sugars, alkali extraction is considered as an effective method for gradually fracti...

    Authors: Shao-Fei Sun, Jing Yang, Da-Wei Wang, Hai-Yan Yang, Shao-Ni Sun and Zheng-Jun Shi
    Citation: Biotechnology for Biofuels 2021 14:72
  13. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Xiao Han, Yi An, Yangyan Zhou, Chao Liu, Weilun Yin and Xinli Xia
    Citation: Biotechnology for Biofuels 2021 14:71

    The original article was published in Biotechnology for Biofuels 2020 13:139

  14. Oxidation and peroxidation of lipids in microorganisms result in increased levels of intracellular reactive oxygen species (ROS) and reactive aldehydes, and consequent reduction of cell growth and lipid accumu...

    Authors: Xiao Han, Zhaohui Li, Ying Wen and Zhi Chen
    Citation: Biotechnology for Biofuels 2021 14:70
  15. Methods to produce XOS have been intensively investigated, including enzymatic hydrolysis, steam explosion, and acid hydrolysis. Acid hydrolysis is currently the most widely used method to produce XOS due to i...

    Authors: Rou Cao, Xinlu Liu, Jianming Guo and Yong Xu
    Citation: Biotechnology for Biofuels 2021 14:69
  16. A single-step ethanol production is the combination of raw cassava starch hydrolysis and fermentation. For the development of raw starch consolidated bioprocessing technologies, this research was to investigat...

    Authors: Morakot Krajang, Kwanruthai Malairuang, Jatuporn Sukna, Krongchan Rattanapradit and Saethawat Chamsart
    Citation: Biotechnology for Biofuels 2021 14:68
  17. Cumulative reported evidence has indicated that renewable feedstocks are a promising alternative source to fossil platforms for the production of fuels and chemicals. In that regard, the development of new, hi...

    Authors: Gabriel Orlando Ferrero, Edgar Maximiliano Sánchez Faba and Griselda Alejandra Eimer
    Citation: Biotechnology for Biofuels 2021 14:67
  18. Epidermal cell walls have special structural and biological roles in the life of the plant. Typically they are multi-ply structures encrusted with waxes and cutin which protect the plant from dehydration and p...

    Authors: Liza A. Wilson, Fabien Deligey, Tuo Wang and Daniel J. Cosgrove
    Citation: Biotechnology for Biofuels 2021 14:66
  19. Crude glycerol (CG) and hemicellulose hydrolysate (HH) are low—value side-products of biodiesel transesterification and pulp—and paper industry or lignocellulosic ethanol production, respectively, which can be...

    Authors: Mikolaj Chmielarz, Johanna Blomqvist, Sabine Sampels, Mats Sandgren and Volkmar Passoth
    Citation: Biotechnology for Biofuels 2021 14:65
  20. The production of microalgal biofuels, despite their sustainable and renowned potential, is not yet cost-effective compared to current conventional fuel technologies. However, the biorefinery concept increases...

    Authors: Gonzalo M. Figueroa-Torres, Jon K. Pittman and Constantinos Theodoropoulos
    Citation: Biotechnology for Biofuels 2021 14:64
  21. Conventional aqueous dilute sulfuric acid (DSA) pretreatment of lignocellulosic biomass facilitates hemicellulose solubilization and can improve subsequent enzymatic digestibility of cellulose to fermentable g...

    Authors: Abhishek S. Patri, Ramya Mohan, Yunqiao Pu, Chang G. Yoo, Arthur J. Ragauskas, Rajeev Kumar, David Kisailus, Charles M. Cai and Charles E. Wyman
    Citation: Biotechnology for Biofuels 2021 14:63
  22. The filamentous fungus Trichoderma reesei is one of the best producers of cellulase and has been widely studied for the production of cellulosic ethanol and bio-based products. We previously reported that Mn2+ an...

    Authors: Yumeng Chen, Xingjia Fan, Xinqing Zhao, Yaling Shen, Xiangyang Xu, Liujing Wei, Wei Wang and Dongzhi Wei
    Citation: Biotechnology for Biofuels 2021 14:62
  23. Textile industry represents one prevalent activity worldwide, generating large amounts of highly contaminated and rich in azo dyes wastewater, with severe effects on natural ecosystems and public health. Howev...

    Authors: Sameh Samir Ali, Rania Al-Tohamy, Eleni Koutra, Michael Kornaros, Maha Khalil, Tamer Elsamahy, Mohamed El-Shetehy and Jianzhong Sun
    Citation: Biotechnology for Biofuels 2021 14:61
  24. Miscanthus is a commercial lignocellulosic biomass crop owing to its high biomass productivity, resilience and photosynthetic capacity at low temperature. These qualities make Miscanthus a particularly good candi...

    Authors: Jose J. De Vega, Abel Teshome, Manfred Klaas, Jim Grant, John Finnan and Susanne Barth
    Citation: Biotechnology for Biofuels 2021 14:60
  25. Pyrolysis-molecular beam mass spectrometry (py-MBMS) analysis of a pedigree of Populus trichocarpa was performed to study the phenotypic plasticity and heritability of lignin content and lignin monomer compositio...

    Authors: Anne E. Harman-Ware, David Macaya-Sanz, Chanaka Roshan Abeyratne, Crissa Doeppke, Kathleen Haiby, Gerald A. Tuskan, Brian Stanton, Stephen P. DiFazio and Mark F. Davis
    Citation: Biotechnology for Biofuels 2021 14:59
  26. Multiple analytical methods have been developed to determine the ratios of aromatic lignin units, particularly the syringyl/guaiacyl (S/G) ratio, of lignin biopolymers in plant cell walls. Chemical degradation...

    Authors: Renee M. Happs, Bennett Addison, Crissa Doeppke, Bryon S. Donohoe, Mark F. Davis and Anne E. Harman-Ware
    Citation: Biotechnology for Biofuels 2021 14:58
  27. Heterotrophic single-cell oils (SCOs) are one potential replacement to lipid-derived biofuels sourced from first-generation crops such as palm oil. However, despite a large experimental research effort in this...

    Authors: Eleni E. Karamerou, Sophie Parsons, Marcelle C. McManus and Christopher J. Chuck
    Citation: Biotechnology for Biofuels 2021 14:57
  28. Slow degradation kinetics of long-chain fatty acids (LCFA) and their accumulation in anaerobic digesters disrupt methanogenic activity and biogas production at high loads of waste lipids. In this study, we eva...

    Authors: Sepehr Shakeri Yekta, Tong Liu, Thuane Mendes Anacleto, Mette Axelsson Bjerg, Luka Šafarič, Xavier Goux, Anna Karlsson, Annika Björn and Anna Schnürer
    Citation: Biotechnology for Biofuels 2021 14:56
  29. Pretreatments are commonly used to facilitate the deconstruction of lignocellulosic biomass to its component sugars and aromatics. Previously, we showed that iron ions can be used as co-catalysts to reduce the...

    Authors: Chien-Yuan Lin, Bryon S. Donohoe, Yannick J. Bomble, Haibing Yang, Manal Yunes, Nicholas S. Sarai, Todd Shollenberger, Stephen R. Decker, Xiaowen Chen, Maureen C. McCann, Melvin P. Tucker, Hui Wei and Michael E. Himmel
    Citation: Biotechnology for Biofuels 2021 14:55
  30. Yellow nutsedge is a unique plant species that can accumulate up to 35% oil of tuber dry weight, perhaps the highest level observed in the tuber tissues of plant kingdom. To gain insight into the molecular mec...

    Authors: Hongying Ji, Dantong Liu and Zhenle Yang
    Citation: Biotechnology for Biofuels 2021 14:54
  31. Sugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high ...

    Authors: Guoquan Liu, Yan Zhang, Hao Gong, Shan Li, Yunrong Pan, Christopher Davis, Hai-Chun Jing, Luguang Wu and Ian D. Godwin
    Citation: Biotechnology for Biofuels 2021 14:53
  32. The amount of carbon dioxide in the atmosphere has been on the rise for more than a century. Bioenergy crops are seen by the Intergovernmental Panel on Climate Change as an essential part of the solution to ad...

    Authors: Obste Therasme, Timothy A. Volk, Mark H. Eisenbies, Thomas E. Amidon and Marie-Odile Fortier
    Citation: Biotechnology for Biofuels 2021 14:52
  33. Lytic polysaccharide monooxygenases (LPMOs) are important industrial enzymes known for their catalytic degradation of recalcitrant polymers such as cellulose or chitin. Their activity can be measured by length...

    Authors: Søren Brander, Stine Lausten, Johan Ø. Ipsen, Kristoffer B. Falkenberg, Andreas B. Bertelsen, Morten H. H. Nørholm, Lars H. Østergaard and Katja S. Johansen
    Citation: Biotechnology for Biofuels 2021 14:51
  34. Hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT) is a central enzyme of the so-called “esters” pathway to monolignols. As originally envisioned, HCT functions twice in this pathway, to form c...

    Authors: Juan Carlos Serrani-Yarce, Luis Escamilla-Trevino, Jaime Barros, Lina Gallego-Giraldo, Yunqiao Pu, Art Ragauskas and Richard A. Dixon
    Citation: Biotechnology for Biofuels 2021 14:50
  35. Ionic liquids (ILs) are promising pretreatment solvents for lignocellulosic biomass, but are largely prepared from petroleum precursors. Benzaldehydes from depolymerized lignin, such as vanillin, syringaldehyd...

    Authors: Shihong Liu, Michael Gonzalez, Celine Kong, Scott Weir and Aaron M. Socha
    Citation: Biotechnology for Biofuels 2021 14:47
  36. Biochemical conversion of lignocellulosic biomass to simple sugars at commercial scale is hampered by the high cost of saccharifying enzymes. Lytic polysaccharide monooxygenases (LPMOs) may hold the key to ove...

    Authors: Adnan Kadić, Anikó Várnai, Vincent G. H. Eijsink, Svein Jarle Horn and Gunnar Lidén
    Citation: Biotechnology for Biofuels 2021 14:46
  37. It is interesting to modify sugar metabolic pathways to improve the productivity of biocatalysts that convert sugars to value-added products. However, this attempt often fails due to the tight control of the s...

    Authors: Toru Jojima, Takafumi Igari, Ryoji Noburyu, Akira Watanabe, Masako Suda and Masayuki Inui
    Citation: Biotechnology for Biofuels 2021 14:45
  38. Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance of woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification of the chemical composition ...

    Authors: Madhavi Latha Gandla, Niklas Mähler, Sacha Escamez, Tomas Skotare, Ogonna Obudulu, Linus Möller, Ilka N. Abreu, Joakim Bygdell, Magnus Hertzberg, Torgeir R. Hvidsten, Thomas Moritz, Gunnar Wingsle, Johan Trygg, Hannele Tuominen and Leif J. Jönsson
    Citation: Biotechnology for Biofuels 2021 14:43
  39. The global demand for functional proteins is extensive, diverse, and constantly increasing. Medicine, agriculture, and industrial manufacturing all rely on high-quality proteins as major active components or p...

    Authors: Jennifer A. Schmidt, Lubna V. Richter, Lisa A. Condoluci and Beth A. Ahner
    Citation: Biotechnology for Biofuels 2021 14:42
  40. Baker’s yeast is a widely used eukaryotic cell factory, producing a diverse range of compounds including biofuels and fine chemicals. The use of lignocellulose as feedstock offers the opportunity to run these ...

    Authors: Friederike Gutmann, Cosimo Jann, Filipa Pereira, Andreas Johansson, Lars M. Steinmetz and Kiran R. Patil
    Citation: Biotechnology for Biofuels 2021 14:41
  41. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Jeffrey P. Tingley, Kristin E. Low, Xiaohui Xing and D. Wade Abbott
    Citation: Biotechnology for Biofuels 2021 14:40

    The original article was published in Biotechnology for Biofuels 2021 14:16

  42. Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial ferment...

    Authors: Nozomu Shibata, Hiroshi Kakeshita, Kazuaki Igarashi, Yasushi Takimura, Yosuke Shida, Wataru Ogasawara, Tohru Koda, Tomohisa Hasunuma and Akihiko Kondo
    Citation: Biotechnology for Biofuels 2021 14:39
  43. Saccharomyces cerevisiae is well-known as an ideal model system for basic research and important industrial microorganism for biotechnological applications. Acetic acid is an important growth inhibitor that has d...

    Authors: Yanfei Cheng, Hui Zhu, Zhengda Du, Xuena Guo, Chenyao Zhou, Zhaoyue Wang and Xiuping He
    Citation: Biotechnology for Biofuels 2021 14:38
  44. Woody plants with high glucose content are alternative bioresources for the production of biofuels and biochemicals. Various pretreatment methods may be used to reduce the effects of retardation factors such a...

    Authors: Dae-Seok Lee, Yoon-Gyo Lee, Eun Jin Cho, Younho Song and Hyeun-Jong Bae
    Citation: Biotechnology for Biofuels 2021 14:37
  45. Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis;...

    Authors: Lin Dai, Tian Huang, Kankan Jiang, Xin Zhou and Yong Xu
    Citation: Biotechnology for Biofuels 2021 14:35