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  1. The sustainable production of biofuels remains one of the major issues of the upcoming years. Among the number of most desirable molecules to be produced, butanol and isobutanol deserve a prominent place. They...

    Authors: Paola Branduardi, Valeria Longo, Nadia Maria Berterame, Giorgia Rossi and Danilo Porro
    Citation: Biotechnology for Biofuels 2013 6:68
  2. Nitrogen limitation can induce neutral lipid accumulation in microalgae, as well as inhibiting their growth. Therefore, to obtain cultures with both high biomass and high lipid contents, and explore the lipid ...

    Authors: Zhi-Kai Yang, Ying-Fang Niu, Yu-Han Ma, Jiao Xue, Meng-Han Zhang, Wei-Dong Yang, Jie-Sheng Liu, Song-Hui Lu, Yuanfang Guan and Hong-Ye Li
    Citation: Biotechnology for Biofuels 2013 6:67
  3. Furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulose biomass to monomeric sugars, but the response of acetone butanol ethanol (ABE) producing Clostridium be...

    Authors: Yan Zhang and Thaddeus Chukwuemeka Ezeji
    Citation: Biotechnology for Biofuels 2013 6:66
  4. Chloroplast transformation in tobacco has been used extensively to produce recombinant proteins and enzymes. Chloroplast expression cassettes can be designed with different configurations of the cis-acting ele...

    Authors: Igor Kolotilin, Angelo Kaldis, Eridan Orlando Pereira, Serge Laberge and Rima Menassa
    Citation: Biotechnology for Biofuels 2013 6:65
  5. Hydrogen recovered from organic wastes and solar energy by photo-fermentative bacteria (PFB) has been suggested as a promising bioenergy strategy. However, the use of PFB for hydrogen production generally suff...

    Authors: Guo-Jun Xie, Bing-Feng Liu, De-Feng Xing, Jun Nan, Jie Ding and Nan-Qi Ren
    Citation: Biotechnology for Biofuels 2013 6:64
  6. Trichoderma reesei is an organism involved in degradation of (hemi)cellulosic biomass. Consequently, the corresponding enzymes are commonly used in different types of industries, and recently gained considerable ...

    Authors: Christian Derntl, Loreta Gudynaite-Savitch, Sophie Calixte, Theresa White, Robert L Mach and Astrid R Mach-Aigner
    Citation: Biotechnology for Biofuels 2013 6:62
  7. The development of affordable woody biomass feedstocks represents a significant opportunity in the development of cellulosic biofuels. Primary woodchips produced by forest mills are considered an ideal feedsto...

    Authors: Aaron M Socha, Samuel P Plummer, Vitalie Stavila, Blake A Simmons and Seema Singh
    Citation: Biotechnology for Biofuels 2013 6:61
  8. α-Pinene is an important natural product that is widely used in flavorings, fragrances, medicines, fine chemicals and high-density renewable fuels. Currently, α-Pinene used in industry is mainly produced eithe...

    Authors: Jianming Yang, Qingjuan Nie, Meng Ren, Hongru Feng, Xinglin Jiang, Yanning Zheng, Min Liu, Haibo Zhang and Mo Xian
    Citation: Biotechnology for Biofuels 2013 6:60
  9. Lignocellulosic ethanol is a viable alternative to petroleum-based fuels with the added benefit of potentially lower greenhouse gas emissions. Consolidated bioprocessing (simultaneous enzyme production, hydrol...

    Authors: Trevor R Zuroff, Salvador Barri Xiques and Wayne R Curtis
    Citation: Biotechnology for Biofuels 2013 6:59
  10. Although α-linked xylose is a major constituent of the hemicelluloses of land plants, few secreted α-xylosidases have been described from fungi or bacteria. AxlA of Aspergillus niger is a secreted α-xylosidase th...

    Authors: Dina Jabbour, Melissa S Borrusch, Goutami Banerjee and Jonathan D Walton
    Citation: Biotechnology for Biofuels 2013 6:58
  11. Second-generation biofuels are generally produced from the polysaccharides in the lignocellulosic plant biomass, mainly cellulose. However, because cellulose is embedded in a matrix of other polysaccharides an...

    Authors: Rebecca Van Acker, Ruben Vanholme, VĂ©ronique Storme, Jennifer C Mortimer, Paul Dupree and Wout Boerjan
    Citation: Biotechnology for Biofuels 2013 6:46
  12. Interest in the detailed lignin and polysaccharide composition of plant cell walls has surged within the past decade partly as a result of biotechnology research aimed at converting biomass to biofuels. High-r...

    Authors: Roger A Chylla, Rebecca Van Acker, Hoon Kim, Ali Azapira, Purba Mukerjee, John L Markley, VĂ©ronique Storme, Wout Boerjan and John Ralph
    Citation: Biotechnology for Biofuels 2013 6:45
  13. The isopentenols, including isoprenol and prenol, are excellent alternative fuels. However, they are not compounds largely accumulated in natural organism. The need for the next generation of biofuels with bet...

    Authors: Yanning Zheng, Qiang Liu, Lingling Li, Wen Qin, Jianming Yang, Haibo Zhang, Xinglin Jiang, Tao Cheng, Wei Liu, Xin Xu and Mo Xian
    Citation: Biotechnology for Biofuels 2013 6:57
  14. The study presented here has used the commercial flow sheeting program Aspen Plus™ to evaluate techno-economic aspects of large-scale hemp-based processes for producing transportation fuels. The co-production ...

    Authors: Zsolt Barta, Emma Kreuger and Lovisa Björnsson
    Citation: Biotechnology for Biofuels 2013 6:56
  15. Biodiesel production has increased dramatically over the last decade, raising the need for new rapid and non-destructive analytical tools and technologies. 1H Low Field Nuclear Magnetic Resonance (LF-NMR) applica...

    Authors: Paula Berman, Adi Leshem, Oren Etziony, Ofer Levi, Yisrael Parmet, Michael Saunders and Zeev Wiesman
    Citation: Biotechnology for Biofuels 2013 6:55
  16. Wheat straw used for bioethanol production varies in enzymatic digestibility according to chemical structure and composition of cell walls and tissues. In this work, the two biologically different wheat straw ...

    Authors: Mads AT Hansen, Budi J Hidayat, Kit K Mogensen, Martin D Jeppesen, Bodil Jørgensen, Katja S Johansen and Lisbeth G Thygesen
    Citation: Biotechnology for Biofuels 2013 6:54
  17. Plant-produced biomass-degrading enzymes are promising tools for the processing of lignocellulose to fermentable sugars. A major limitation of in planta production is that high-level expression of such enzymes co...

    Authors: Holger Klose, Markus Günl, Björn Usadel, Rainer Fischer and Ulrich Commandeur
    Citation: Biotechnology for Biofuels 2013 6:53
  18. Ionic liquid (IL) pretreatment has shown great potential as a novel pretreatment technology with high sugar yields. To improve process economics of pretreatment, higher biomass loading is desirable. The goal o...

    Authors: Alejandro G Cruz, Chessa Scullin, Chen Mu, Gang Cheng, Vitalie Stavila, Patanjali Varanasi, Dongyan Xu, Jeff Mentel, Yi-De Chuang, Blake A Simmons and Seema Singh
    Citation: Biotechnology for Biofuels 2013 6:52
  19. Reducing the production cost of, and increasing revenues from, industrial biofuels will greatly facilitate their proliferation and co-integration with fossil fuels. The cost of feedstock is the largest cost in...

    Authors: Michael E Pyne, Murray Moo-Young, Duane A Chung and C Perry Chou
    Citation: Biotechnology for Biofuels 2013 6:50
  20. In recent years biogas plants in Germany have been supposed to be involved in amplification and dissemination of pathogenic bacteria causing severe infections in humans and animals. In particular, biogas plant...

    Authors: Felix G Eikmeyer, Antje Rademacher, Angelika Hanreich, Magdalena Hennig, Sebastian Jaenicke, Irena Maus, Daniel Wibberg, Martha Zakrzewski, Alfred PĂĽhler, Michael Klocke and Andreas SchlĂĽter
    Citation: Biotechnology for Biofuels 2013 6:49
  21. n-Butanol and isobutanol produced from biomass-derived sugars are promising renewable transport fuels and solvents. Saccharomyces cerevisiae has been engineered for butanol production, but its high butanol sensit...

    Authors: Daniel González-Ramos, Marcel van den Broek, Antonius JA van Maris, Jack T Pronk and Jean-Marc G Daran
    Citation: Biotechnology for Biofuels 2013 6:48
  22. Caldicellulosiruptor saccharolyticus is a thermophilic, Gram-positive, non-spore forming, strictly anaerobic bacterium of interest in potential industrial applications, including the production of biofuels such a...

    Authors: Nancy G Isern, Junfeng Xue, Jaya V Rao, John R Cort and Birgitte K Ahring
    Citation: Biotechnology for Biofuels 2013 6:47
  23. A key step in any process that converts lignocellulose to biofuels is the efficient fermentation of both hexose and pentose sugars. The co-culture of respiratory-deficient Saccharomyces cerevisiae and wild-type S...

    Authors: Timothy J Hanly and Michael A Henson
    Citation: Biotechnology for Biofuels 2013 6:44
  24. Delignification pretreatments of biomass and methods to assess their efficacy are crucial for biomass-to-biofuels research and technology. Here, we applied confocal and fluorescence lifetime imaging microscopy...

    Authors: Vitor Carlos Coletta, Camila Alves Rezende, Fernando Rodrigues da Conceição, Igor Polikarpov and Francisco Eduardo Gontijo Guimarães
    Citation: Biotechnology for Biofuels 2013 6:43
  25. Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid ...

    Authors: Kun Wang, Haiyan Yang, Wei Wang and Run-cang Sun
    Citation: Biotechnology for Biofuels 2013 6:42
  26. It is widely believed that reducing the lignocellulosic biomass particle size would improve the biomass digestibility by increasing the total surface area and eliminating mass and heat transfer limitation duri...

    Authors: Shuhaida Harun, Venkatesh Balan, Mohd Sobri Takriff, Osman Hassan, Jamaliah Jahim and Bruce E Dale
    Citation: Biotechnology for Biofuels 2013 6:40
  27. The use of Ionic liquids (ILs) as biomass solvents is considered to be an attractive alternative for the pretreatment of lignocellulosic biomass. Acid catalysts have been used previously to hydrolyze polysacch...

    Authors: Ning Sun, Hanbin Liu, Noppadon Sathitsuksanoh, Vitalie Stavila, Manali Sawant, Anaise Bonito, Kim Tran, Anthe George, Kenneth L Sale, Seema Singh, Blake A Simmons and Bradley M Holmes
    Citation: Biotechnology for Biofuels 2013 6:39
  28. Understanding the effects of pretreatment on anaerobic digestion of sludge waste from wastewater treatment plants is becoming increasingly important, as impetus moves towards the utilization of sludge for rene...

    Authors: Mabel Ting Wong, Dong Zhang, Jun Li, Raymond Kin Hi Hui, Hein Min Tun, Manreetpal Singh Brar, Tae-Jin Park, Yinguang Chen and Frederick C Leung
    Citation: Biotechnology for Biofuels 2013 6:38
  29. Microalgal biomass contains a high level of carbohydrates which can be biochemically converted to biofuels using state-of-the-art strategies that are almost always needed to employ a robust pretreatment on the...

    Authors: Byong-Hun Jeon, Jeong-A Choi, Hyun-Chul Kim, Jae-Hoon Hwang, Reda AI Abou-Shanab, Brian A Dempsey, John M Regan and Jung Rae Kim
    Citation: Biotechnology for Biofuels 2013 6:37
  30. Microbial lipid production by using lignocellulosic biomass as the feedstock holds a great promise for biodiesel production and biorefinery. This usually involves hydrolysis of biomass into sugar-rich hydrolys...

    Authors: Zhiwei Gong, Hongwei Shen, Qian Wang, Xiaobing Yang, Haibo Xie and Zongbao K Zhao
    Citation: Biotechnology for Biofuels 2013 6:36
  31. Currently, the most promising microorganism used for the bio-production of butyric acid is Clostridium tyrobutyricum ATCC 25755T; however, it is unable to use sucrose as a sole carbon source. Consequently, a newl...

    Authors: Mohammed Dwidar, Seil Kim, Byoung Seung Jeon, Youngsoon Um, Robert J Mitchell and Byoung-In Sang
    Citation: Biotechnology for Biofuels 2013 6:35
  32. The development of inhibitor-tolerant ethanologenic yeast is one of the most significant challenges facing bio-ethanol production. Adaptation of Pichia stipitis to inhibitors is one of the most efficient ways for...

    Authors: Xue-Cai Hao, Xiu-Shan Yang, Ping Wan and Shen Tian
    Citation: Biotechnology for Biofuels 2013 6:34
  33. The investigation of structural organisation in lignocellulose materials is important to understand changes in cellulase accessibility and reactivity resulting from hydrothermal deconstruction, to allow develo...

    Authors: Roger Ibbett, Sanyasi Gaddipati, Sandra Hill and Greg Tucker
    Citation: Biotechnology for Biofuels 2013 6:33
  34. Cellulose is highly recalcitrant and thus requires a specialized suite of enzymes to solubilize it into fermentable sugars. In C. thermocellum, these extracellular enzymes are present as a highly active multi-com...

    Authors: Devin H Currie, Christopher D Herring, Adam M Guss, Daniel G Olson, David A Hogsett and Lee R Lynd
    Citation: Biotechnology for Biofuels 2013 6:32
  35. Consolidated bioprocessing (CBP) of lignocellulosic biomass to ethanol using thermophilic bacteria provides a promising solution for efficient lignocellulose conversion without the need for additional cellulol...

    Authors: Vitali A Svetlitchnyi, Oliver Kensch, Doris A Falkenhan, Svenja G Korseska, Nadine Lippert, Melanie Prinz, Jamaleddine Sassi, Anke Schickor and Simon Curvers
    Citation: Biotechnology for Biofuels 2013 6:31
  36. A vast number of organisms are known to produce structurally diversified cellulases capable of degrading cellulose, the most abundant biopolymer on earth. The generally accepted paradigm is that the carbohydra...

    Authors: Anikó Várnai, Matti Siika-aho and Liisa Viikari
    Citation: Biotechnology for Biofuels 2013 6:30
  37. Production of biodiesel from non-edible oils is receiving increasing attention. Tung oil, called “China wood oil” is one kind of promising non-edible biodiesel oil in China. To our knowledge, tung oil has not ...

    Authors: Xiao-Wei Yu, Chong Sha, Yong-Liang Guo, Rong Xiao and Yan Xu
    Citation: Biotechnology for Biofuels 2013 6:29
  38. A substantial barrier to commercialization of lignocellulosic ethanol production is a lack of process specific sensors and associated control strategies that are essential for economic viability. Current senso...

    Authors: Shannon M Ewanick, Wesley J Thompson, Brian J Marquardt and Renata Bura
    Citation: Biotechnology for Biofuels 2013 6:28
  39. β-Xylosidase is an important constituent of the hemicellulase system and it plays an important role in hydrolyzing xylooligosaccharides to xylose. Xylose, a useful monose, has been utilized in a wide range of ...

    Authors: Hao Shi, Xun Li, Huaxiang Gu, Yu Zhang, Yingjuan Huang, Liangliang Wang and Fei Wang
    Citation: Biotechnology for Biofuels 2013 6:27
  40. Xylanase is an important component of hemicellulase enzyme system. Since it plays an important role in the hydrolysis of hemicellulose into xylooligosaccharides (XOs), high thermostable xylanase has been the f...

    Authors: Hao Shi, Yu Zhang, Xun Li, Yingjuan Huang, Liangliang Wang, Ye Wang, Huaihai Ding and Fei Wang
    Citation: Biotechnology for Biofuels 2013 6:26
  41. Bacterial cellulose (BC) is a highly crystalline and mechanically stable nanopolymer, which has excellent potential as a material in many novel applications, especially if it can be produced in large amounts f...

    Authors: Adnan Cavka, Xiang Guo, Shui-Jia Tang, Sandra Winestrand, Leif J Jönsson and Feng Hong
    Citation: Biotechnology for Biofuels 2013 6:25
  42. Understanding the biological mechanisms used by microorganisms for plant biomass degradation is of considerable biotechnological interest. Despite of the growing number of sequenced (meta)genomes of plant biom...

    Authors: Aaron Weimann, Yulia Trukhina, Phillip B Pope, Sebastian GA Konietzny and Alice C McHardy
    Citation: Biotechnology for Biofuels 2013 6:24
  43. The use of lignocellulosic constituents in biotechnological processes requires a selective separation of the main fractions (cellulose, hemicellulose and lignin). During diluted acid hydrolysis for hemicellulo...

    Authors: João Paulo Alves Silva, Livia Melo Carneiro and Inês Conceição Roberto
    Citation: Biotechnology for Biofuels 2013 6:23
  44. Pretreatment of biomass for lignocellulosic ethanol production generates compounds that can inhibit microbial metabolism. The furan aldehydes hydroxymethylfurfural (HMF) and furfural have received increasing a...

    Authors: Magnus Ask, Maurizio Bettiga, Valeria Mapelli and Lisbeth Olsson
    Citation: Biotechnology for Biofuels 2013 6:22
  45. Reduced yields of ethanol due to bacterial contamination in fermentation cultures weaken the economics of biofuel production. Lactic acid bacteria are considered the most problematic, and surveys of commercial...

    Authors: Dwayne R Roach, Piyum A Khatibi, Kenneth M Bischoff, Stephen R Hughes and David M Donovan
    Citation: Biotechnology for Biofuels 2013 6:20
  46. Many microorganisms possess enzymes that can efficiently degrade lignocellulosic materials, but do not have the capability to produce a large amount of ethanol. Thus, attempts have been made to transform such ...

    Authors: Jui-Jen Chang, Feng-Ju Ho, Cheng-Yu Ho, Yueh-Chin Wu, Yu-Han Hou, Chieh-Chen Huang, Ming-Che Shih and Wen-Hsiung Li
    Citation: Biotechnology for Biofuels 2013 6:19